A5072586243- Signaling Pathways in Disease
- Finite Group Theory Research
- graph theory and CDMA systems
- Coding theory and cryptography
- Cell Image Analysis Techniques
- Renal and related cancers
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Bioinformatics and Genomic Networks
- Microtubule and mitosis dynamics
- Ion channel regulation and function
- Machine Learning in Bioinformatics
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Gene expression and cancer classification
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- AI in cancer detection
- Renal cell carcinoma treatment
The University of Queensland
2013-2024
University of Vermont
2023-2024
Michigan Technological University
2021-2024
Schrodinger (United States)
2024
University of Rochester
2023
Queensland Cyber Infrastructure Foundation
2022
Northwest Nazarene University
2018-2020
Brisbane School of Theology
2013
University College London
2013
Max Planck Society
2010
The retromer is a trimeric cargo-recognition protein complex composed of Vps26, Vps29 and Vps35 associated with trafficking within endosomes. Recently, pathogenic point mutation the subunit (D620N) was linked to manifestation Parkinson's disease (PD). Here, we investigated details underlying molecular mechanism by which D620N in modulates function, including examination retromer's subcellular localization its capacity sort cargo. We show that expression PD-linked mutant redistributes...
The genomic revolution has led to rapid growth in sequencing of genes and proteins, attention is now turning the function encoded proteins. In this respect, microscope imaging a protein's sub-cellular localisation proving invaluable, recent advances automated fluorescent microscopy allow protein localisations be imaged high throughput. Hence there need for large scale computational techniques efficiently quantify, distinguish classify images. While image statistics have proved highly...
Abstract Background Lateral genetic transfer can lead to disagreements among phylogenetic trees comprising sequences from the same set of taxa. Where topological discordance is thought have arisen through events, tree comparisons be used identify lineages that may shared information. An 'edit path' one or more events represented with a series subtree prune and regraft (SPR) operations, but finding optimal such operations NP-hard for between rooted trees, so unrooted as well. Results...
LOCATE is a curated, web-accessible database that houses data describing the membrane organization and subcellular localization of mouse human proteins. Over past 2 years, in have grown substantially. The now contains high-quality for 20% proteome general annotation nearly 36% proteome. annotated from RIKEN FANTOM Consortium Isoform Protein Sequence sets which 58 128 64 637 protein isoforms. Other additions include computational predictions, automated classification experimental image data,...
We report that phosphoinositol-binding sorting nexin 5 (SNX5) associates with newly formed macropinosomes induced by EGF stimulation. used the recruitment of GFP-SNX5 to track their maturation. Initially, is sequestered discrete subdomains macropinosome; these are subsequently incorporated into highly dynamic, often branched, tubular structures. Time-lapse videomicroscopy revealed dynamic extension SNX5-labelled tubules and departure from macropinosome body follow predefined paths towards...
Rab GTPases including Rab27a, Rab38 and Rab32 function in melanosome maturation or trafficking melanocytes. A screen to identify additional Rabs involved these processes revealed the localization of GFP-Rab17 on recycling endosomes (REs) melanosomes melanocytic cells. Rab17 mRNA expression is regulated by microphthalmia transcription factor (MITF), a characteristic known pigmentation genes. siRNA knockdown melanoma cells quantitatively increased concentration at cell periphery. did not...
Progenitor self-renewal and differentiation is often regulated by spatially restricted cues within a tissue microenvironment. Here, we examine how progenitor cell migration impacts regionally induced commitment the nephrogenic niche in mice. We identify subset of cells that express Wnt4, an early marker nephron commitment, but migrate back into population where they accumulate over time. Single RNA-seq computational modelling returning reveals progenitors can traverse transcriptional...
Glutathione (GSH) is a critical component of the cellular redox system that combats oxidative stress. The glutamate-cystine antiporter, x
Despite the lipolysis–lipogenesis cycle being a fundamental process in adipocyte biology, very little is known about morphological changes that occur during this process. The remodeling of lipid droplets to form micro (mLDs) striking feature lipolysis adipocytes, but once ceases, cell must regain its basal morphology. We characterized mLD formation cultured and primary adipocytes isolated from mouse epididymal fat pads, response acute activation lipolysis. Using real-time quantitative...
Abstract At over 200 years, the maximum lifespan of bowhead whale exceeds that all other mammals. The is also second-largest animal on Earth, reaching 80,000 kg 1 . Despite its very large number cells and long lifespan, not highly cancer-prone, an incongruity termed Peto’s Paradox 2 This phenomenon has been explained by evolution additional tumor suppressor genes in larger animals, supported research elephants demonstrating expansion p53 gene 3–5 Here we show fibroblasts undergo oncogenic...
Abstract We describe a new method for using neural networks to predict residue contact pairs in protein. The main inputs the network are set of 25 measures correlated mutation between all residues two “windows” size 5 centered on interest. While individual pair‐wise correlations relatively weak predictor contact, by training windows correlation accuracy prediction is significantly improved. trained 100 proteins and then tested disjoint 1033 known structure. An average predictive 21.7%...
IL-10 is a key anti-inflammatory cytokine secreted by activated macrophages as feedback control mechanism to prevent excessive inflammatory responses. Here, we define multiple intracellular trafficking pathways involved in the secretion of newly synthesized from following TLR4 activation with LPS, and show how this relates previously defined for IL-6 TNF simultaneously producing these proinflammatory cytokines. exits Golgi tubular carriers, including those dependent on p230GRIP. Some then...
Markov clustering (MCL) is becoming a key algorithm within bioinformatics for determining clusters in networks. However,with increasing vast amount of data on biological networks, performance and scalability issues are critical limiting factor applications. Meanwhile, GPU computing, which uses CUDA tool implementing massively parallel computing environment the card, very powerful, efficient, low-cost option to achieve substantial gains over CPU approaches. The use on-chip memory efficiently...