A5100417364- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Aquaculture Nutrition and Growth
- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Crustacean biology and ecology
- Genetic and phenotypic traits in livestock
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Physiological and biochemical adaptations
- Cardiovascular and Diving-Related Complications
- Identification and Quantification in Food
- Animal Nutrition and Physiology
- Molecular Biology Techniques and Applications
- Pregnancy and preeclampsia studies
- Microbial Metabolism and Applications
- Bat Biology and Ecology Studies
- Anesthesia and Pain Management
- Reproductive biology and impacts on aquatic species
- Nausea and vomiting management
- Microbial Metabolites in Food Biotechnology
- Maternal and fetal healthcare
China Three Gorges University
2024
Qingdao National Laboratory for Marine Science and Technology
2019-2024
Chinese Academy of Fishery Sciences
2019-2024
Chinese Center For Disease Control and Prevention
2024
Chengdu Women's and Children's Central Hospital
2023-2024
Fudan University Shanghai Cancer Center
2021-2023
Weifang Medical University
2023
Qinghai University Affiliated Hospital
2023
Qinghai Institute for Endemic Diease Prevention and Control
2023
Qinghai University
2023
ABSTRACT The Ribosomal Database Project (RDP) Classifier, a naïve Bayesian classifier, can rapidly and accurately classify bacterial 16S rRNA sequences into the new higher-order taxonomy proposed in Bergey's Taxonomic Outline of Prokaryotes (2nd ed., release 5.0, Springer-Verlag, New York, NY, 2004). It provides taxonomic assignments from domain to genus, with confidence estimates for each assignment. majority classifications (98%) were high estimated (≥95%) accuracy (98%). In addition...
Ribosomal Database Project (RDP; http://rdp.cme.msu.edu/) provides the research community with aligned and annotated rRNA gene sequence data, along tools to allow researchers analyze their own sequences in RDP framework. data are utilized fields as diverse human health, microbial ecology, environmental microbiology, nucleic acid chemistry, taxonomy phylogenetics. In addition collections of bacterial archaeal small subunit genes, now includes a collection fungal large genes. tools, including...
The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along analysis services a phylogenetically consistent taxonomic framework for these data.Updated monthly, are made available through RDP-II website (http:// rdp.cme.msu.edu/).RDP-II release 9.21 (August 2004) contains 101 632 bacterial small subunit sequences in format.Highthroughput tools initial placement, identification of related probe primer testing, data navigation...
High-throughput molecular technologies can profile microbial communities at high resolution even in complex environments like the intestinal microbiota. Recent improvements next-generation sequencing allow for finer resolution. We compared phylogenetic profiling of both longer (454 Titanium) sequence reads with shorter, but more numerous, paired-end (Illumina). For approaches, we targeted six tandem combinations 16S rRNA gene variable regions, DNA extracted from a human faecal sample, order...
Metagenomics can provide important insight into microbial communities. However, assembling metagenomic datasets has proven to be computationally challenging. Current methods often assemble only fragmented partial genes. We present a novel method for targeting assembly of specific protein-coding This combines de Bruijn graph, as used in standard approaches, and protein profile hidden Markov model (HMM) the gene interest, annotation approaches. These are create combined weighted graph. Xander...
The importance of nitric oxide (NO) for CBF variations associated with arterial carbon dioxide changes was investigated in halothane-anesthetized rats by using an inhibitor synthase, N G -nitro-l-arginine (NOLAG). measured intracarotid injection 133 Xe. In normocapnia, infusion 1.5, or 7.5, 30 mg/kg NOLAG induced a dose-dependent increase blood pressure and decrease normocapnic from 85 ± 10 to 78 6, 64 5, 52 5 ml 100g −1 min , respectively. This effect lasted at least 2 h. Raising P co...
Abstract Background Impatiens L. is a genus of complex taxonomy that belongs to the family Balsaminaceae (Ericales) and contains approximately 1000 species. The well known for its economic, medicinal, ornamental, horticultural value. However, knowledge about germplasm identification, molecular phylogeny, chloroplast genomics limited, taxonomic uncertainties still exist due overlapping morphological features insufficient genomic resources. Results We sequenced genomes six different species (...
CUEDC2, a CUE-domain-containing protein, modulates inflammation, but its involvement in tumorigenesis is still poorly understood. Here, we report that CUEDC2 key regulator of macrophage function and critical for protection against colitis-associated tumorigenesis. expression dramatically upregulated during differentiation, deficiency results excessive production proinflammatory cytokines. The level macrophages modulated by miR- 324-5p. We find Cuedc2 KO mice are more susceptible to...
<p>Crustacea is a species-rich subphylum of Arthropoda that contains mostly aquatic species. Here, we established comprehensive genomic database dedicated to the Crustacea. CRUSTADB represents significant advancement in crustacean genomics, integrating an extensive array resources. The encompasses 33 latest genomes, providing foundational resource for genetic and studies within this clade. Additionally, it includes impressive 789 Gb resequencing data across 10 species order Decapoda,...
Abstract Chemogenomic profiling is a powerful and unbiased approach to elucidate pharmacological targets the mechanism of bioactive compounds. Until recently, genome-wide, high-resolution experiments this nature have been limited fungal systems due lack mammalian genome-wide deletion collections. With example novel nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, we demonstrate that CRISPR/Cas9 system enables generation transient homo- heterozygous libraries allows for...
A high-quality reference genome is necessary to determine the molecular mechanisms underlying important biological phenomena; therefore, in present study, a chromosome-level assembly of Chinese shrimp Fenneropenaeus chinensis was performed. Muscle male sequenced using PacBio platform, and assembled by Hi-C technology. The F. 1.47 Gb with contig N50 472.84 Kb, including 57.73% repetitive sequences, anchored 43 pseudochromosomes, scaffold 36.87 Mb. In total, 25,026 protein-coding genes were...
Marsupenaeus japonicus is an important marine crustacean species. However, a lack of genomic resources hinders the use whole genome sequencing to explore their genetic basis and molecular mechanisms for genome-assisted breeding. Consequently, we determined chromosome-level M. japonicus. Here determine assembly with total 665.19 Gb data, yielding approximately1.54 contig N50 size 229.97 kb scaffold 38.27 Mb. With high-throughput chromosome conformation capture (Hi-C) technology, anchored...
Eyestalk ablation is an effective method to promote ovarian development in crustaceans. Herein, we performed transcriptome sequencing of ovary and hepatopancreas tissues after eyestalk Exopalaemon carinicauda identify genes related development. Our analyses led the identification 97,383 unigenes 190,757 transcripts, with average N50 length 1757 bp. In ovary, four pathways oogenesis three oocyte rapid growth were enriched. hepatopancreas, two vitellogenesis-associated transcripts identified....
Most of our knowledge about translation regulatory mechanisms comes from studies on lower organisms. However, the control system higher organisms is less understood. Here we find that in 5΄ untranslated region (5΄UTR) human Annexin II receptor (AXIIR) mRNA, there are two upstream open reading frames (uORFs) acting a fail-safe manner to inhibit main AUG. These uORFs unfavorable for re-initiation after termination uORF translation. Heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1),...
Abstract Objectives Clear cell renal carcinoma (cc RCC ) is characterized histologically by accumulation of cholesterol esters, and other neutral lipids. Lysosomal acid lipase ( LAL a critical enzyme involved in the ester metabolism. Here, we sought to determine whether could orchestrate metabolism esters order promote cc progression. Materials methods Quantitative reverse‐transcription PCR western blots were conducted assess expression human tissues. We analysed relationship between levels...
Vitellogenin (Vtg) is a precursor of yolk proteins in egg-laying vertebrates and invertebrates plays an important role vitellogenesis embryonic development. However, the Vtg family remains poorly characterized Exopalaemon carinicauda, major commercial mariculture species found along coasts Yellow Bohai Seas. In this study, 10 genes from genomes E. carinicauda were identified characterized. Phylogenetic analyses showed that crustaceans could be classified into four groups: Astacidea,...
The Stripe-Backed Shrew, Sorex cylindricauda belongs to the family Soricidae, and distributes in northwestern Yunnan, central Sichuan, southern Gansu Shaanxi. In this study, complete mitochondrial genome sequence of S. was determined. mitogenome is 17,191 bp length contains 13 protein-coding genes, 2 ribosomal RNA 22 transfer genes 1 control region, with a base composition 33.2% A, 30.2% T, 23.8% C 12.8% G. study contributes illuminating taxonomic status Shrew cylindricauda.
Abstract Although many phenotypic traits of chickens have been well documented, the genetic patterns gene expression levels in remain to be determined. In present study, we crossed two chicken breeds, White Leghorn (WL) and Cornish (Cor), which selected for egg meat production, respectively, a few hundred years. We evaluated transcriptome abundance brain, muscle, liver from day-old progenies pure-bred WL Cor, hybrids these by RNA-Seq order determine inheritance expression. Comparison among...
Pear ring rot disease, mainly caused by Botryosphaeria dothidea, is widespread in most pear and apple-growing regions. Mycoviruses are used for biocontrol, especially fruit tree disease. BdCV1 (Botryosphaeria dothidea chrysovirus 1) BdPV1 partitivirus influence the biological characteristics of B. strains. a potential candidate control fungal Therefore, it vital to explore interactions between mycovirus clarify pathogenic mechanisms hypovirulence pear. A high-quality full-length genome...
Abstract Background Gene expression variation is a key underlying factor influencing phenotypic variation, and can occur via cis- or trans-regulation. To understand the role of trans-regulatory on population divergence in chicken, we developed reciprocal crosses two chicken breeds, White Leghorn Cornish Game, which exhibit major differences body size reproductive traits, used them to determine degree cis versus trans brain, liver, muscle tissue male female 1-day-old specimens. Results We...
To discern the symptomatic features of coronavirus disease 2019 (COVID-19) and to evaluate severity prognosis disease.