A5067751497- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Tea Polyphenols and Effects
- Plant Disease Resistance and Genetics
- Essential Oils and Antimicrobial Activity
- Nematode management and characterization studies
- Fungal and yeast genetics research
- Photonic and Optical Devices
- Legume Nitrogen Fixing Symbiosis
- Phytochemicals and Antioxidant Activities
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Probiotics and Fermented Foods
- Wheat and Barley Genetics and Pathology
- Advanced Fiber Optic Sensors
- Plant tissue culture and regeneration
- Potato Plant Research
- Plant and fungal interactions
- Transgenic Plants and Applications
- Bacillus and Francisella bacterial research
- Evolution and Genetic Dynamics
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Bacteriophages and microbial interactions
Xinyang Normal University
2017-2025
Huazhong Agricultural University
2012-2022
Chinese Academy of Agricultural Sciences
2020
Institute of Plant Protection
2020
Controlling toxigenic Fusarium graminearum (FG) is challenging. A bacterial strain (S76-3, identified as Bacillus amyloliquefaciens) that was isolated from diseased wheat spikes in the field displayed strong antifungal activity against FG. Reverse-phase high performance liquid chromatography and electrospray ionization mass spectrometry analyses revealed S76-3 produced three classes of cyclic lipopeptides including iturin, plipastatin surfactin. Each class consisted several different...
Aflatoxigenic Aspergillus fungi and associated aflatoxins are ubiquitous in the production storage of food/feed commodities. Controlling these microbes is a challenge. In this study, Shewanella algae strain YM8 was found to produce volatiles that have strong antifungal activity against pathogens. Gas chromatography-mass spectrometry profiling revealed 15 volatile organic compounds (VOCs) emitted from YM8, which dimethyl trisulfide most abundant. We obtained authentic reference standards for...
Abstract Background Catechins, caffeine, and theanine as three important metabolites in the tea leaves play essential roles formation of specific taste shows potential health benefits to humans. However, knowledge on dynamic changes these content over seasons, well candidate regulatory factors, remains largely undetermined. Results An integrated transcriptomic metabolomic approach was used analyze mainly including catechins, theanine, explore influencing factors associated with course...
Combined analyses of the natural occurrence fusarium head blight (FHB), mycotoxins and mycotoxin‐producing isolates Fusarium spp. in fields wheat revealed FHB epidemics 12 14 regions Hubei 2009. Mycotoxin contamination ranged from 0·59 to 15·28 μg g −1 grains. Of causal agents associated with symptoms FHB, 84% were asiaticum 9·5% graminearum , while remaining 6·5% other species. Genetic chemotyping demonstrated that F. comprised deoxynivalenol (DON), 3‐acetyldeoxynivalenol (3‐AcDON),...
Globally, the trichothecene mycotoxins deoxynivalenol (DON) and nivalenol (NIV) are among most widely distributed that contaminate small grain cereals. In this study, a bacterial consortium, PGC-3, with de-epoxydation activity was isolated from soil by an in situ enrichment method. Screening of 14 samples were sprayed DON revealed 4 able to biotransform into de-epoxydized (dE-DON). Among these, PGC-3 consortium showed highest stable dE-DON NIV dE-NIV. exhibited at wide range pH (5–10)...
Controlling aflatoxigenic Aspergillus flavus and aflatoxins (AFs) in grains food during storage is a great challenge to humans worldwide. Alcaligenes faecalis N1-4 isolated from tea rhizosphere soil can produce abundant antifungal volatiles, greatly inhibited the growth of A. un-contacted face-to-face dual culture testing. Gas chromatography tandem mass spectrometry revealed that dimethyl disulfide (DMDS) methyl isovalerate (MI) were two compounds volatile profiles N1-4. DMDS was found have...
Aspergillus flavus and the produced aflatoxins cause great damage to crop production, food security, human health. The control of A. in grains during storage is a challenge humans worldwide. In this study, we investigated potential strain TR-18, isolated from rhizosphere tea plants, controlling aflatoxins. results demonstrated that TR-18 greatly inhibited growth dual cultural tests by production antifungal volatiles. was identified as Flavobacterium johnsoniae through biochemical analysis...
Abstract A recently isolated Fusarium population from maize in Belgium was identified as a new species, temperatum . From survey of species associated with ear rot nineteen provinces 2009 China, ten strains Guizhou and Hubei were F. Morphological molecular phylogenetic analyses based on the DNA sequences individual translation elongation factor 1‐alpha β‐tubulin genes revealed that recovered isolates produced macroconidia typical four‐septate foot‐shaped basal cell belonged to is distinctly...
Fungal secreted proteins that contain the Common in Extracellular Membrane (CFEM) domain are important for pathogenicity. The hemibiotrophic fungus Colletotrichum graminicola causes serious anthracnose disease of maize. In this study, we identified 24 CgCFEM genome C. graminicola. Phylogenic analysis revealed these (CgCFEM1—24) can be divided into 2 clades based on presence trans-membrane domain. Sequence alignment indicated amino acids CFEM highly conserved and 8 spaced cysteines, with...
Abstract Background Soil fertility decline and pathogen infection are severe issues for crop production all over the world. Microbes as inherent factors in soil were effective alleviating decrease, promoting plant growth controlling pathogens et al. Thus, screening microbes with improving properties is of great importance to humans. Results Bacteria Pt-3 isolated from tea rhizosphere showed multiple functions solubilizing insoluble phosphate, growth, producing abundant volatile organic...
Maize stalk rot (MSR) caused by Fusarium graminearum is the primary factor contributing to reduction in maize yield and quality. However, this soil-borne disease presents a significant challenge for sustainable control through field management chemical agents. The screening of novel biocontrol agents can aid developing innovative successful strategies MSR control.
A sensitive and specific analytical method to detect ubiquitous aflatoxigenic Aspergillus pathogens is essential for monitoring controlling aflatoxins. Four highly reactive chicken single-chain variable fragments (scFvs) against soluble cell wall proteins (SCWPs) from flavus were isolated by phage display. The scFv antibody AfSA4 displayed the highest activity toward both A. parasiticus specifically recognized a surface target of their walls as revealed immunofluorescence localization....
Abstract Fusarium graminearum clade species are among the main causative agents of Gibberella ear rot (GER) in maize and responsible for various trichothecene mycotoxins accumulated contaminated grains. In this study, a total 620 isolates from diseased ears collected 59 districts 19 provinces throughout China, previously identified morphologically as clade, was genetically characterized at level based on SCAR (Sequence Characterized Amplified Region) their potential capability mycotoxin...
Aspergillus flavus and the produced aflatoxins cause great hazards to food security human health across all countries. The control of A. in grains during storage is significance humans. In current study, bacteria strain YM6 isolated from sea sediment was demonstrated effective controlling by production anti-fungal volatiles. According morphological characteristics phylogenetic analysis, identified as Pseudomonas stutzeri. can produce abundant volatile compounds which could inhibit mycelial...
Escherichia coli bacteria have been found to be responsible for various health outbreaks caused by contaminated food and water. Accurate rapid test of E. is thus crucial protecting the public health. A fast‐response, label‐free bacteriophage‐based detection using multimode microfiber probe proposed demonstrated in this article. Due abrupt taper subwavelength diameter, different modes are excited guided as evanescent field that can interact with surrounding directly. The change concentration...
Controlling proliferation and aflatoxin production by Aspergillus flavus is a pressing challenge for global food safety security. Marine bacterium Staphylococcus saprophyticus strain L-38 showed excellent antifungal activity toward A. in vitro vivo . In sealed, non-contact confrontation assays, completely inhibited conidial germination mycelial growth of through the volatile organic compounds (VOCs). Gas chromatography-mass spectrometry identified 3,3-dimethyl-1,2-epoxybutane (3-DE) as most...
As one of the world's top three popular non-alcoholic beverages, tea is economically and culturally valuable. Xinyang Maojian, this elegant green tea, ten famous in China has gained prominence for thousands years. However, cultivation history Maojian population selection signals differentiation from other major variety Camellia sinensis var. assamica (CSA) remain unclear. We newly generated 94 (C. sinensis) transcriptomes including 59 samples area 35 collected 13 planting provinces China....