A5027866645- Animal Virus Infections Studies
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Parvovirus B19 Infection Studies
- Respiratory viral infections research
- Livestock and Poultry Management
- Hepatitis B Virus Studies
- Microbial infections and disease research
- Viral Infections and Outbreaks Research
- Plant Virus Research Studies
- Hepatitis Viruses Studies and Epidemiology
Teva Pharmaceuticals (Hungary)
2023
Hungarian Academy of Sciences
2020
Biologicals (Czechia)
2004
A neurological disease of young Pekin ducks characterized by ataxia, lameness, and paralysis was observed at several duck farms in Malaysia 2012. Gross pathological lesions were absent or inconsistent most the cases, but severe consistent microscopic found brain spinal cord, non-purulent panencephalomyelitis. Several virus isolates obtained embryonated eggs cell cultures (Vero DF-1) inoculated with homogenates affected ducks. After exclusion other viruses, identified as a flavivirus...
Newcastle disease (ND) is a highly contagious of chickens causing significant economic losses worldwide. Due to the limitation in their efficacy, current vaccination strategies against ND need improvements. This study aimed evaluate new-generation vaccine for its efficacy providing clinical protection and reducing virus shedding after challenge. Broiler were vaccinated ovo or subcutaneously at hatch with turkey herpesvirus-based recombinant (rHVT) expressing key protective antigen (F...
In countries where avian influenza has become endemic, early vaccination of layer pullets or broilers with classical inactivated vaccines at the hatchery is no longer an option because interference passive immunity indirectly induced by necessary breeders. On other hand, injection thousands chicks from 7 to 10 days old on farms been determined be unreliable and, therefore, poorly efficacious. For these reasons, interest arisen regarding a newly developed live recombinant vector vaccine based...
Polymerase chain reaction and sequencing were used to analyse goose parvovirus field isolates vaccine strains. Two fragments of the genome amplified. Fragment "A" represents a region VP3 gene, while fragment "B" upstream encompassing part VP1 gene. In deduced amino acid sequence strains was identical, therefore differentiation among could be done only at nucleotide level, which resulted in formation three groups: Hungarian, West-European Asian "B", separation groups by both level. The...
Avian reoviruses (ARV), an important pathogen of poultry, have received increasing interest lately due to their widespread occurrence, recognized genetic diversity, and association defined disease conditions or being present as co-infecting agents. The efficient control measures require the characterization available virus strains.The study describes ARV collection comprising over 200 isolates from diagnostic samples collected a decade 34 countries worldwide. One hundred thirty-six were...
Outbreaks caused by the highly pathogenic avian influenza virus (HPAIV) H5N8 subtype clade 2.3.4.4 were first reported in 2014 South Korea then spread very rapidly Asia, to Europe, and for time, North America. Efficacy of a recombinant HVT-AI (H5) vaccine (rHVT-H5) provide clinical protection as well significantly reduce shedding an challenge has already been demonstrated SPF chickens. The aim our studies was test efficacy same rHVT-H5 controlling transmission recent Hungarian HPAIV...
Routine molecular diagnostic testing by our laboratory, based on using a primer pair with conservative binding sites the spike glycoprotein coding sequence, has indicated recurring of unique phylogenetic cluster chicken infectious bronchitis viruses (IBV) in Middle East since 2010. The nearly full-length S1 subunit gene phylogeny selected strains, however, split up this grouping, suggesting potential recombination gene. In order to clarify this, various bioinformatic analyses strains were...
The control of Newcastle disease (ND) highly relies on vaccination. Immunity provided by a ND vaccine can be characterized measuring the level clinical protection and reduction in challenge virus shedding. extent shedding depends lot characteristics used quality vaccination, but influenced also genotype virus. We demonstrated that vaccination SPF chicks with recombinant herpesvirus turkey expressing F-gene I (rHVT-ND) complete against heterologous VII.1.1 strain reduced significantly. 100%...
Waterfowl play a key role in the epidemiology of H5N1 subtype highly pathogenic avian influenza (HPAI) virus; therefore, efficient immunization domesticated ducks and geese to maximize impact other control measures is great importance. A recombinant (r)HVT-AI, expressing HA gene clade 2.2 HPAI strain had been developed proved be against different clades virus chickens after single vaccination at 1 day old could provide long-term immunity. We investigated whether rHVT-AI applied able...
Newcastle Disease is one of the most important infectious poultry diseases worldwide and associated with high morbidity, mortality, economic loss. In several countries, vaccination applied to prevent control outbreaks; however, information on ability vaccines reduce transmission ND virus (NDV) sparse. Here we quantified velogenic NDV among 42-day-old broilers. Chickens were either vaccinated a single dose vector vaccine expressing F protein (rHVT-ND) at day-old in presence maternally derived...
The most recent pandemic clade of highly pathogenic avian influenza (HPAI) H5, 2.3.4.4, spread widely, with the involvement wild birds, importantly waterfowl, carrying virus (even asymptomatically) from Asia to North America, Europe, and Africa. Domestic waterfowl being in regular contact birds played a significant role H5Nx epizootics. Therefore, protection domestic infection would likely cut transmission chain these viruses greatly enhance efforts control prevent disease outbreak other...
Newcastle disease (ND) is still a major poultry worldwide. Vaccination remains the principal method of controlling ND in endemic countries. Various vaccination strategies, including use recently developed recombinant vaccines, have been used to control it. Recombinant vaccines that herpesvirus turkey (HVT) as vector express one key antigens virus (NDV) overcome some drawbacks related conventional vaccines. HVT appears unique beneficial characteristics: it extremely safe, not affected by...
The antigenic characterization of IBDV, a virus that causes an immunosuppressive disease in young chickens, has been historically addressed using cross neutralization (VN) assay and antigen-capture enzyme-linked immunosorbent (AC-ELISA). However, VN usually carried out either specific antibody negative embryonated eggs, for non-cell culture adapted strains, which is tedious, or on chicken embryo fibroblasts (CEF), requires adaptation to cell culture. AC-ELISA provided crucial information...
Abstract During 2014–2017, we isolated a novel orthobunyavirus from broiler chickens with severe kidney lesions in the state of Kedah, Malaysia; named virus Kedah fatal syndrome (KFKSV). Affected became listless and diarrheic before dying suddenly. Necropsies detected pale swollen kidneys signs gout, enlarged fragile livers, hearts. Experimental infection KFKSV reproduced disease pathologic conditions observed field, fulfilling Koch's postulates. Gene sequencing indicated high nucleotide...
Effective control of infectious bronchitis is a challenge in commercial poultry operations due to the high transmissibility virus. Although multiple IBV lineages are circulating United States, DMV1639-type strain (GI-17) currently major variant, creating production losses industry. This study aimed test whether combination GA08 (GI-27) and Mass-type (GI-1) IB vaccines could significantly reduce transmission field 4-week-old broilers. Half birds were directly challenged, whereas other half...
A H9N2 altípusú influenzavírus növekvő elterjedtsége, gazdasági, járványtani, humánegészségügyi jelentősége miatt a szerzők irodalmi adatok alapján összefoglalják, rendszerezik az altípussal kapcsolatos aktuális ismereteket. Napjainkban madárinfluenza-vírusok közül altípusúak legelterjedtebbek baromfifajokban. Bár korábban vadmadárvonulások, valamint élőállatpiacok voltak fertőződés forrásai, elmúlt évtizedekben már baromfihoz adaptálódott vírusok egyre nagyobb földrajzi területen váltak...