A5076093419- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Respiratory viral infections research
- Virology and Viral Diseases
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Animal Nutrition and Physiology
- Livestock and Poultry Management
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Agricultural pest management studies
- Microbial infections and disease research
- Cytomegalovirus and herpesvirus research
- COVID-19 epidemiological studies
- Aquaculture disease management and microbiota
- Protein Structure and Dynamics
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Moringa oleifera research and applications
- Virus-based gene therapy research
- Mollusks and Parasites Studies
Animal Health Research Institute
2010-2025
Zagazig University
2025
Agricultural Research Center
2014-2024
Animal Health Institute
2021
Central Laboratory For Agricultural Expert Systems
2008-2019
Port Said University
2019
Beni-Suef University
2019
Friedrich-Loeffler-Institut
2017
Food and Agriculture Organization of the United Nations
2016
Département Santé Animale
2010
ABSTRACT H5N1 highly pathogenic avian influenza virus has been endemic in poultry Egypt since 2008, notwithstanding the implementation of mass vaccination and culling infected birds. Extensive circulation resulted a progressive genetic evolution an antigenic drift. In poultry, occurrence drift viruses is less well documented mechanisms remain to be clarified. To test hypothesis that driven by similar type A humans, we generated reassortant viruses, reverse genetics, harbored molecular...
The epidemiology of foot-and-mouth disease (FMD) in North Africa is complicated by the co-circulation endemic FMD viruses (FMDV), as well sporadic incursions exotic viral strains from Middle East and Sub-Saharan Africa. This report describes molecular characterization SAT 2 that have caused widespread field outbreaks Egypt during February March 2012. Phylogenetic analysis showed these fell into two distinct lineages within topotype VII, which were a contemporary lineage same toptype Libya....
We isolated highly pathogenic avian influenza virus (H5N8) of clade 2.3.4.4 from the common coot (Fulica atra) in Egypt, documenting its introduction into Africa through migratory birds. This has a close genetic relationship with subtype H5N8 viruses circulating Europe. Enhanced surveillance to detect newly emerging is warranted.
Abstract The role of Africa in the dynamics global spread a zoonotic and economically-important virus, such as highly pathogenic avian influenza (HPAI) H5Nx Gs/GD lineage, remains unexplored. Here we characterise spatiotemporal patterns virus diffusion during three HPAI intercontinental epidemic waves demonstrate that mainly acted an ecological sink viruses. A joint analysis host continuous spatial indicates poultry trade well wild bird migrations have contributed to spreading into Africa,...
This paper describes the first threats of H5N1 avian influenza outbreaks in Egypt recorded from February to December 2006 commercial and domestic poultry different species summarizes major characteristics outbreak. There were 1024 cases (rural chickens breeding types, turkeys, ducks, geese, quail) either or backyards locations Egypt. All tested positive for subtype. From these only 12 A viruses isolated characterized samples collected during outbreaks. isolates characterized, data confirmed...
Highly pathogenic avian influenza virus A/H5N1 was first officially reported in Africa early 2006. Since the outbreak Nigeria, this spread rapidly to other African countries. From its emergence 2008, 11 countries experienced outbreaks poultry and human cases were also three of these At present, little is known epidemiology molecular evolution viruses Africa. We have generated 494 full gene sequences from 67 isolates applied analysis tools a total 1,152 obtained isolated Africa, Europe Middle...
In this paper, we describe results from a high-pathogenic H5N1 avian influenza virus (AIV) surveillance program in previously H5-vaccinated commercial and family-backyard poultry flocks that was conducted 2007 to 2008 by the Egyptian National Laboratory for Veterinary Quality Control on Poultry Production. The real-time reverse transcription PCR assay used detect A matrix gene detection of H5 N1 subtypes accomplished using commercially available kit assay. detected 35/3,610 (0.97%) 27/8,682...
A distinct cluster of highly pathogenic avian influenza viruses subtype A(H5N1) has been found to emerge within clade 2.2.1.2 in poultry Egypt since summer 2014 and appears have quickly become predominant. Viruses this may be associated with increased incidence human infections over the last months.
Wild migratory birds have the capability to spread avian influenza virus (AIV) over long distances as well transmit domestic birds. In this study, swab and tissue samples were obtained from 190 during close surveillance in Egypt response recent outbreaks of highly pathogenic (HPAI) H5N1 virus. The collected tested for a variety AIV subtypes (H5N1, H9N2, H5N8, H6N2) other pathogens such NDV, IBV, ILT, IBDV, WNV. Among all samples, HPAI was found six samples; be negative pathogens. Egyptian...
The poultry meat trade in Egypt depends mainly on live bird markets (LBMs) because of insufficient slaughterhouses, lack marketing infrastructure, and cultural preference for consumption freshly slaughtered poultry. There are two types LBMs Egypt: retail shops traditional where minimal, if any, food safety standards or veterinary inspection implemented. Before January 2009, were considered to be a missing link the epidemiology avian influenza Egypt. This incited us initiate this surveillance...
One of the major problems avian infectious bronchitis virus (IBV) is frequent emergence new variants. In present study 205 tracheal swabs and organs were collected from broilers layers chicken farms during January to August 2012 19 governorates all over Egypt. The chickens demonstrated respiratory signs mortality. Out examined samples, 130 which (about 64%) suspected positive for IBV with real time RT-PCR. 13 IBV-positive samples selected further isolation characterization. Isolation in...
Methanolic extract of Capparis sinaica Veill was tested for its in vitro antiviral activity against highly pathogenic avian influenza strain H5N1 using plaque inhibition assay Madin–Darby canine kidney. The results indicated that the possessed potent (100% at concentration 1 μg/ml). Based on this result, C. selected further study by applying bioactivity-guided fractionation to isolate principles. fractions eluted with EtOAc and 25% MeOH were found hold activity. Further chromatographic...
Re-emergence of highly virulent forms IBDV has been the cause significant economic losses. In present study, 52 bursa samples were assayed using reverse transcriptase-polymerase chain reaction (RT-PCR) for targeting VP2 gene. Out tested 20 positives. Eleven IBDV-positive selected further isolation and characterization. Histopathological analysis revealed necrosis, presence depleted follicles some infiltration heterophils, characteristic to previously reported in IBDV. The virus was isolated...
Abstract Background The endemic H5N1 high pathogenicity avian influenza virus (A/H5N1) in poultry Egypt continues to cause heavy losses and poses a significant threat human health. Methods Here we describe results of A/H5N1 surveillance domestic 2009 wild birds 2009–2010. Tracheal cloacal swabs were collected from 22024 commercial farms, 1435 backyards 944 live bird markets (LBMs) as well 1297 representing 28 different types migratory birds. Viral RNA was extracted mix tracheal media. Matrix...
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype are widely distributed within poultry populations in Egypt and have caused multiple human infections. Linking epidemiological sequence data is important to understand transmission, persistence evolution virus. This work describes phylogenetic dynamics based on molecular characterization hemagglutinin (HA) gene isolates collected from February 2006 May 2014. Full-length HA sequences 368 were generated genetically analysed...
Highly pathogenic avian influenza A virus (H5N1) has diverged antigenically and genetically since its initial detection in Asia 1997. Viruses belonging to clade 2.2 particular have been reported numerous countries with the majority occurring Egypt. Previous reports identified antigenic similarities between viruses 2.2. However, poultry human isolated northern Egypt during 2007 2008 were found be distinct from other this country. Genetic analysis of hemagglutinin revealed a high degree...
The Eurasian-lineage H5N1 highly pathogenic avian influenza (HPAI) virus caused widespread outbreaks in Egypt 2006 and eventually become enzootic poultry. Although have a seasonal pattern, with most occurring during the cooler winter months, it remains unclear whether this seasonality reflects maintenance within or yearly introductions of into country. To evaluate epidemiology HPAI Egypt, sequence analysis hemagglutinin (HA) neuraminidase (NA) genes selected Egyptian isolates from early to...
Widespread prevalence of avian influenza H9N2 subtype in the Middle East region and its detection Egypt quail early summer 2011 added another risk factor to Egyptian poultry industry addition highly pathogenic H5N1 subtype. This situation increases need for further surveillance investigation viruses commercial household chickens. work describes genetic characterization recently isolated from chicken flocks. Parallel infections has also been done compare two subtypes close geographic...