A5022551814- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Immune Response and Inflammation
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Respiratory viral infections research
- Viral Infectious Diseases and Gene Expression in Insects
- Vibrio bacteria research studies
- RNA Interference and Gene Delivery
- Antibiotic Resistance in Bacteria
- interferon and immune responses
- Salmonella and Campylobacter epidemiology
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
Agricultural Research Center
2024-2025
Ministry of Agriculture and Land Reclamation
2010-2022
Animal Health Research Institute
2010-2020
Food and Agriculture Organization of the United Nations
2015-2019
Département Santé Animale
2016
Quality Research
2016
Central Laboratory For Agricultural Expert Systems
2016
World Health Organization - Egypt
2016
Highly pathogenic avian influenza (HPAI) H5 subtype remains a significant menace to both the poultry industry and human public health. Biosecurity mass vaccination of susceptible commercial flocks are crucial reduce devastating economic loss hinder evolution virus. In this study, we developed multivalent virus (AIV) vaccine, including strains representing HPAI 2.2.1.1., 2.2.1.2., 2.3.4.4b clades circulating in Egypt Middle East. Specific pathogen-free (SPF) two-week-old chickens were...
A distinct cluster of highly pathogenic avian influenza viruses subtype A(H5N1) has been found to emerge within clade 2.2.1.2 in poultry Egypt since summer 2014 and appears have quickly become predominant. Viruses this may be associated with increased incidence human infections over the last months.
The poultry meat trade in Egypt depends mainly on live bird markets (LBMs) because of insufficient slaughterhouses, lack marketing infrastructure, and cultural preference for consumption freshly slaughtered poultry. There are two types LBMs Egypt: retail shops traditional where minimal, if any, food safety standards or veterinary inspection implemented. Before January 2009, were considered to be a missing link the epidemiology avian influenza Egypt. This incited us initiate this surveillance...
Abstract Background The endemic H5N1 high pathogenicity avian influenza virus (A/H5N1) in poultry Egypt continues to cause heavy losses and poses a significant threat human health. Methods Here we describe results of A/H5N1 surveillance domestic 2009 wild birds 2009–2010. Tracheal cloacal swabs were collected from 22024 commercial farms, 1435 backyards 944 live bird markets (LBMs) as well 1297 representing 28 different types migratory birds. Viral RNA was extracted mix tracheal media. Matrix...
Six vaccination regimes using classical (Mass-type) and variant (IB-VAR2 IB-793B) live vaccines were evaluated against Middle Eastern variant-2 infectious bronchitis virus challenge. groups of SPF chicks (30 birds/group) vaccinated prime-boost at day-1 day-14 using; IB-M41:IB-VAR2, IB-VAR2:IB-VAR2, IB-VAR2:IB-M41, IB-Ma5:IB-793B, IB-793B:IB-793B, IB-793B:IB-Ma5, respectively. Ciliostasis lesion scores day-5 after each vaccination. Birds challenged intranasally 14-day post 2nd 105EID50/0.1...
A total of 50 poultry farms commercial broilers (N = 39) and layers 11) suffered from respiratory problems mortality during the period January 2016 to December 2017 were investigated. Also, samples collected quail 4), Bluebird (Sialis, N 1), Greenfinch (Chloris chloris, 1) for analysis. Respiratory viral pathogens screened by PCR positive subjected virus isolation genetic identification. Antigenic relatedness isolated avian influenza (AI) H5 subtype was evaluated using cross-hemagglutination...
Vaccination is the main tool implemented in Egypt since 2007 to control H5N1 avian influenza. The present study aimed at comparing effectiveness of three influenza vaccination regimes commercial broiler chickens carrying high levels maternally derived antibodies (MDAs). Day-old chicks were divided into four experimental groups. Group I received only rHVT-H5 vaccine (recombinant turkey herpesvirus (HVT) which carries a H5 clade 2.2 insert) administered D1. II KV-H5 (an oil emulsion killed...
Vaccination against H5N1 highly pathogenic avian influenza (AI) virus (HPAIV) is one of the possible complementary means available for affected countries to control AI when disease has become, or with a high risk becoming, endemic. Efficacy vaccination relies essentially, but not exclusively, on capacity vaccine induce immunity targeted (which prone undergo antigenic variations), as well its overcome interference maternal transmitted by immunized breeding hens their progeny. This property...
The effectiveness of recombinant turkey herpesvirus avian influenza (A/swan/Hungary/4999/2006(H5N1)) clade 2.2 virus (rHVT-H5) vaccine was evaluated in two layer chicken breeds (White Bovans [WB] and Brown Shaver [BS]). One dose rHVT-H5 administered at day 1 birds were monitored serologically (haemagglutination inhibition test) virologically for 19 weeks. Maternally-derived antibody post-vaccination H5 titres measured using the Chinese (A/Goose/Guangdong/1/96(H5N1)) HA Egyptian...
Vaccination programs against infectious bronchitis virus (IBV) in Egypt depend on both classical and/or imported variant IBV strain vaccines. However, many outbreaks associated with respiratory distress, nephropathy, and high mortalities were attributed to the circulation of new nephropathogenic 2 strains. In present study, we report development attenuated candidate vaccines using classic strains (IBM41 IB2) a (IBvar2). The wild-type (WT) viruses through serial passages embryonated specific...
This study evaluated the efficacy of live and inactivated conventional GII LaSota recombinant GVII Newcastle disease vaccines in commercial broilers. The experimental groups (G2-G7) were vaccinated on day 7 21 age with from same vaccine type "GII LaSota, (A), (B)" via eye drop; however, G3, G5, G7 received a single dose counterpart subcutaneously age. Vaccine was based elicited humoral immunity, clinical protection, reduction virus shedding after challenge virulent 1.1. strain. Results...
Avian influenza A (H5N1) virus is one of the most important public health concerns worldwide. The antiviral activity native and esterified whey proteins fractions (α- lactalbumin, β- lactoglobulin, lactoferrin) was evaluated against A/chicken/Egypt/086Q-NLQP/2008 HPAI strain clade 2.2.1 (for multiplicity infection (1 MOI) after 72 h incubation at 37 °C in presence 5% CO₂) using MDCK cell lines.Both lactoferrin seem to be active protein among tested samples, followed by lactoglobulin....
In May 2009, during routine monitoring of a commercial layer flock about 87,000 birds kept in cages 4 different houses that had been vaccinated 3 times with an inactivated H5N1 vaccine at weeks 1, 7, and 16, highly pathogenic avian influenza (HPAI) virus subtype was isolated detected by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) tracheal cloacal swabs collected from 4; 7 days after onset clinical signs, there increase mortality accompanied decrease egg production...
Uninterrupted transmission of highly pathogenic avian influenza virus (HPAIV) H5N1 clade 2.2.1 in Egypt since 2006 resulted establishment two main genetic clusters. The 2.2.1/C group where all recent human and majority backyard origin viruses clustered together, meanwhile the derived from vaccinated poultry commercial farms grouped 2.2.1.1 clade.In present investigation, an HPAIV was isolated twenty weeks old layers chickens that were with a homologous vaccine at 1, 7 16 old. At age, birds...
Since the first report of low pathogenic avian influenza (LPAI) H9N2 virus in Egypt 2011, Egyptian poultry industry has suffered from unexpected economic losses as a result wide spread LPAI H9N2. Hence, inactivated vaccines have been included vaccination programs different production sectors. The optimal antigen content is essential to reach protective antibody titers. In this study, correlation between (based on hemagglutinating units [HAU]) and postvaccination (PV) response vaccine was...
Egyptian poultry suffer from frequent respiratory disease outbreaks associated with Infectious Bronchitis Virus (IBV) variant 2 strains (Egy/VarII). Different vaccination programs using imported vaccines have failed to protect the flocks field challenge. Recent studies confirmed a successful protection homologous as live attenuated vaccines. In this study, newly developed IB-VAR2 vaccine representing GI-23 Middle East IBV lineage was evaluated in day-old commercial broilers an IBV-endemic...
In Egypt, ducks kept for commercial purposes constitute the second highest poultry population, at 150 million ducks/year. Hence, play an important role in introduction and transmission of avian influenza (AI) Egyptian population. Attempts to control outbreaks include use vaccines, which have varying levels efficacy failure. To date, effects vaccine has rarely been determined ducks. this study, we evaluated protective a live recombinant vector based on turkey Herpes Virus (HVT) expressing H5...
In late 2016, a highly pathogenic avian influenza (HPAI) virus subtype H5N8 clade 2.3.4.4 was reported in Egypt migratory birds; subsequently, the spread to backyard and commercial poultry several Egyptian governorates, causing severe economic losses industry. Here, recombinant subunit H5 vaccine prepared from 2.3.2 segment on baculovirus evaluated Pekin ducks (Anasplatyrhynchos domesticus) Muscovy (Cairina moschata) Biosafety Level 3 isolators by using two vaccination regimes: either single...
Extensive vaccination against Newcastle disease virus (NDV) induced more selective immune pressure from hosts that enhanced the evolutionary process of NDV. Herein, we characterized 13 recently isolated NDV isolates vaccinated chicken flocks during 2014–2017. Sequence analysis F gene showed presence 112RRQKRF117 velogenic cleavage motif in 11 isolates, whereas other two (Ck/ME3/Eg/16 and Ck/ME5/Eg/16) monobasic 112GGRQGRL117. Interestingly, isolate Ck/ME5/Eg/16 100% 99.82% nucleotide...
In the last 40 years, low pathogenic avian influenza virus (LPAIV) subtype H9N2 has been endemic in most Middle Eastern countries and of course Egypt which is one biggest poultry producers middle east region. The major losses with infections come from complicated commercial broiler chickens, especially E. coli infection. this work, 2,36,345 Arbor acres chickens same breeder flock were placed equally four pens, where two pens vaccinated against LPAIV virus, other served as non-vaccinated...