A5062171563- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Animal Virus Infections Studies
- Salmonella and Campylobacter epidemiology
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Animal Nutrition and Physiology
- Plant Virus Research Studies
- Clostridium difficile and Clostridium perfringens research
- Veterinary medicine and infectious diseases
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial Resistance in Staphylococcus
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Rabbits: Nutrition, Reproduction, Health
- Yersinia bacterium, plague, ectoparasites research
- Moringa oleifera research and applications
- Identification and Quantification in Food
- Bacillus and Francisella bacterial research
- Chemical Analysis and Environmental Impact
- Trace Elements in Health
- Livestock and Poultry Management
Animal Health Research Institute
2014-2024
Agricultural Research Center
2020-2023
Ministry of Agriculture and Land Reclamation
2014-2015
Highly pathogenic avian influenza (HPAI) H5N1 and H5N8 have become endemic among domestic poultry in Egypt since 2006 2016, respectively. In parallel, the low H9N2 virus has been 2010. Despite continuous circulation of these subtypes for several years, no natural reassortant detected so far population Egypt. this study, HPAI (H5N2) was isolated from a commercial duck farm, giving evidence emergence first reassortment event The derived as result genetic between viruses circulating exchange...
Background and Aim: Multidrug resistance (MDR) of Escherichia coli has become an increasing concern in poultry farming worldwide. However, E. can accumulate genes through gene transfer. The most problematic mechanism is the acquisition encoding broad-spectrum β-lactamases, known as extended-spectrum that confer to cephalosporins. Plasmid-mediated quinolone (conferring quinolones) mcr-1 colistin) also contribute antimicrobial resistance. This study aimed investigate prevalence susceptibility...
The highly pathogenic avian influenza (HPAI) H5N8 virus was first detected in Egypt late 2016. Since then, the has spread rapidly among different poultry sectors, becoming dominant HPAI H5 subtype reported Egypt. Different genotypes of were Egypt; however, geographic patterns and molecular evolution Egyptian viruses are still unclear. Here, extensive epidemiological surveillance conducted, including more than half a million samples collected from sectors (farms/backyards/live bird markets)...
Background and Aim: Rabbit hemorrhagic disease (RHD) is an economically important disorder of rabbits, where infection results in severe losses to the meat fur industries. Our goal was characterize RHD virus (RHDV) strains currently circulating different regions Egypt. Materials Methods: Fifty rabbits suspected harboring RHDV from 15 Egyptian governorates were evaluated. Diseased identified by clinical signs postmortem lesions. confirmed through hemagglutination assay (HA) polymerase chain...
The pathogenic potential of vancomycin and methicillin-resistant coagulase-negative Staphylococci (VMRCoNS) on Egyptian poultry farms has received little attention. Therefore, this study aims to the prevalence CoNS in imported flocks commercial farms, evaluate presence virulence antibiotic resistance genes (sea, seb, sec, sed, see, mecA), assess their pathogenicity broiler chicks. Seven species were identified among 25 isolates, such as 8 S. gallinarum, 5 saprophyticus, chromogens, 3...
In Egypt, ducks kept for commercial purposes constitute the second highest poultry population, at 150 million ducks/year. Hence, play an important role in introduction and transmission of avian influenza (AI) Egyptian population. Attempts to control outbreaks include use vaccines, which have varying levels efficacy failure. To date, effects vaccine has rarely been determined ducks. this study, we evaluated protective a live recombinant vector based on turkey Herpes Virus (HVT) expressing H5...
Bacteriophages have been mainly used in treating infections caused by planktonic bacterial cells the veterinary sector. However, their applications as antibiofilm agents received little attention. Accordingly, a previously isolated Salmonella infecting Siphoviridae phage was investigated for host range against 15 enterica isolates (S. Cape, S. Gallinarum, 4 Enteritidis, 3 Montevideo, Uno, Oritamerin, Belgdam, Agona, Daula, and Aba) recovered from litters of commercial broiler farms. All were...
Orange peel and its extract are good sources of phenols vitamin C that can be used as powerful antioxidants antibacterial. The effects dietary ascorbic acid (AA), orange powder (OPP) (OPE) supplementations on growth performance, blood biochemicals, gene expression antioxidant status growing rabbits under hot conditions were investigated. A total 80 weaned Giant Flander male rabbits, five weeks old (606.25 ± 10.08 g), randomly assigned to four groups. first group received untreated diet...
Considering that highly pathogenic avian influenza H5N1 endemically circulates in the Egyptian poultry population, Active surveillance was undertaken 195 broiler and layers farms from Eighteen governorates resulted in, 34,7 24 positive for AI H9N2, Virulent ND respectively, using RT-PCR.The genetic analysis of viruses HA gene type revealed these are strains as they tracking clade 2.2.1 classic which became endemic Egypt since 2006.However, H9N2 low G1-lineage Asian transmitted to Middle East...
Colistin is considered the lastly used antimicrobial drug for treatment of many infections.As extensive use colistin, resistance has been increasing rapidly, thus, 200 different chicken samples were examined isolation E. coli and colistin gene.Eighty-three selected (chicken breast) subjected to detection residues by HPLC (High-performance liquid chromatography).In this research, we polymerase chain reaction (PCR) mcr-1 gene, phylogeny was performed on three isolates detect gene mutations...
Flinders Technology Associates (FTA) cards simplify sample storage, transport, and extraction by reducing cost time for diagnosis. This study evaluated the FTA suitability safe transport storage of Gram-positive Gram-negative bacterial cells animal origin on its liquid culture form from organ impression smears (tissues) under same routine condition microbiological laboratory along with detecting their nucleic acid over different conditions.Increase in count 104 to 107 (colony-forming...
Campylobacter Jejuni is one of the Gram-negative bacteria causing worldwide foodborne illness, especially from poultry species. Human infection may be raised as a consumption uncooked infected poultry. This study evaluates occurrence jejuni in different commercial species (chickens, ducks, and pigeons) its virulence. In this study, organs were collected pooled together (liver intestine) chickens, pigeons Egyptian provinces. The total isolation percentage for was 26.4% highest ducks 36%...
Bacterial pathogens play an important role in causing respiratory diseases poultry. Six pathogens; Escherichia coli, Mycoplasma gallisepticum, Staphylococcus aureus, Pasteurella multocida, Mannheimia haemolytica and Pseudomonas aeruginosa are known to cause infections However, current diagnostic methods for them not highly sensitive, laborious time consuming. This research aimed develop a multiplex polymerase chain reaction (m-PCR) that could detect the six species clinical specimens from...
Rabbits are a highly sensitive species and susceptible to various bacterial pathogens that may be causative agents for early embryonic death. This study aimed explore the administration of different in does suffering from Furthermore, identification genes associated with virulence was performed identify phenotypic genotypic antimicrobial resistance patterns increase lead death.We isolated identified 106 samples live dead female rabbits had undergone death, including liver intestine tissue,...
Resistance to flruroquinolones encouraged us study the resistance of E. coli isolates from avian origin in Egypt. A total 65 150 samples were positive for E was screened against 5 quinolones discs and also five representing genes (qnrA, qnrB, qnrS, qepA aac(6′)-Ib-cr). The absolute three out antimicrobial appeared duck isolates; chicken showed 100% nalidixic acid as totally higher percentage examined especially that has role decreasing sensitivity nofloxacin, cibrofloxacin enrofloxacin....
Bacteriological examination of 166 different flocks collected from 62, 52 and imported duck, chick poult respectively, revealed that 15, 8 14 were positive for S.aureus isolation with percentage 24.2%, 15.4% 26.9% respectively. In-vitro antimicrobial susceptibility testing isolates was studied using disc diffusion method. All Staphylococcus aureus subjected to molecular detection PCR confirm the results isolation. This data focusing on newly hatched poultry represent a risk introducing S....
Campylobactercoli and Campylobacter jejuni may cause gastrointestinal disorders with or without necrotic hepatitis in poultry serious foodborne enteritis sometimes fatal consequences humans. Little is known about the prevalence of spp. ducks, particularly young ducklings.In this study, 36 (24%) isolates Campylobacterspp. were isolated from 150 samples 1-day-old ducklings Egypt. Using biochemical tests specific PCR, 33 C. coli 3 identified. Allisolates sensitive to chloramphenicol amikacin...
In a surveillance targeting different birds communities, 237 cloacal swabs and intestinal samples from 39 species of wild sources were examined bacteriologically for Salmonella.32 positive Salmonella spp., with an incidence 13.5%.Migratory had the highest 28.26 percentage.The in free living was somewhat lower (18.57%).While Zoo lowest (4.96%).Serotyping isolates revealed 6 serotypes [Salmonella Typhimurium, Rissen, Regent, Doncaster, Curacao, IIIb group (O65) untyped Salmonella] .Salmonella...
Differentiation between field and vaccinal strains of Mycoplasma gallisepticumis important for the epidemiology disease.Real time PCR assay was applied to detect MG further tested by mgc2 rt-PCR F, 6/85 TS-11 strains.Twenty four farms were positive Out 50 farms, from which 12 F strain 5 strain, while 7 but negative live vaccine rt-PCR.DNA nucleotide sequencing 20 cases gene confirmed results, some point mutations found in isolated grouped sequenced samples into 4 groups (F, two named group A B).
N ecrotic enteritis caused by Clostridium perfringens has been linked to severe economic losses in the poultry industry and represents a serious threat public health due spore-forming ability its role as foodborne pathogen.Although chemical disinfection plays preventing controlling spread of bacterial diseases livestock housing, little is known about efficiency against C. perfringens.This study was designed investigate sporicidal activity combination quaternary ammonium glutaraldehyde...