Escherichia coli is an important zoonotic bacterium that significantly impacts one health concept. E. normally detected in the gut of warm-blooded animals, but some serotypes can cause diseases humans and animals. Moreover, it continue for a long time different environments, replicate water, survive outside hosts. In this study, 171 samples collected from 10 types poultry hatcheries (automatic, semiautomatic, manual "traditional" types) were examined prevalence coli. PCR was applied to...

Campylobacter is considered to be the most leading bacterial cause of human gastroenteritis worldwide. Consumption undercooked or contaminated chicken food main source campylobacteriosis. Although a gastroenteritis, data on comparative virulence gene profiles jejuni (C. jejuni) and coli coli) from sources in Egypt remain scarce. This study aimed characterize genes both C. isolated fecal samples Ismailia governorate, Egypt. A total 20 isolates each species were screened for 15 genes. All...

Abstract Bovine coronavirus (BCoV) is a common viral enteric pathogen responsible for diarrhea in newborn calves. Despite its economic significance, there limited research on this virus Egypt. This study aimed to detect the prevalence of BCoV, associated risk factors, and biochemical changes during infection. A cross-sectional included 196 pre-weaned diarrheic calves chosen randomly from 16 farms. Fecal samples were obtained these calves, questionnaire was administered investigate positivity...

Highly pathogenic avian influenza (HPAI) H5N1 and H5N8 have become endemic among domestic poultry in Egypt since 2006 2016, respectively. In parallel, the low H9N2 virus has been 2010. Despite continuous circulation of these subtypes for several years, no natural reassortant detected so far population Egypt. this study, HPAI (H5N2) was isolated from a commercial duck farm, giving evidence emergence first reassortment event The derived as result genetic between viruses circulating exchange...

Background and Aim: Multidrug resistance (MDR) of Escherichia coli has become an increasing concern in poultry farming worldwide. However, E. can accumulate genes through gene transfer. The most problematic mechanism is the acquisition encoding broad-spectrum β-lactamases, known as extended-spectrum that confer to cephalosporins. Plasmid-mediated quinolone (conferring quinolones) mcr-1 colistin) also contribute antimicrobial resistance. This study aimed investigate prevalence susceptibility...

Highly pathogenic H5N1 avian influenza virus (A/H5N1) of clade 2.2.1 is endemic in poultry Egypt where the highest number human infections worldwide was reported. During last 12 years Egyptian A/H5N1 evolved into several genotypes. In 2007-2014 vaccinated suffered from antigenic drift variants 2.2.1.1 and 2014/2015 an unprecedented upsurge 2.2.1.2 occurred humans. Factors contributing to endemicity or re-emergence remain unclear. Here, three potential factors were studied: climatic...

Fowl adenoviruses (FAdVs) are a large group of viruses different serotypes. They responsible for inclusion body hepatitis, adenoviral gizzard erosion, and hepatitis hydropericardium syndrome. The present study presents comprehensive overview FAdVs in Egypt, with focus on the epidemiological features virus serotypes across country. We conducted molecular investigation multiple FAdV species based genetic signature hypervariable regions 1-4 loop1 (L1) region hexon gene. Epidemiologically, Nile...

Abstract Background Avian influenza viruses (AIVs) have been identified from more than 100 different species of wild birds around the globe. Wild migratory can act as potential spreaders for AIVs to domestic between countries. Egypt is situated on important flyways continents. While much known about circulation zoonotic H5N1 and H9N2 in poultry Egypt, little pivotal role maintenance transmission Egypt. Methods Targeted AIV surveillance has conducted 2017 wetlands areas Northern Eastern...

Highly pathogenic avian influenza (HPAI) viruses continue to circulate worldwide, causing numerous outbreaks among bird species and severe public health concerns. H5N1 H5N8 are the two most fundamental HPAI subtypes detected in birds last decades. The may compete with each other while sharing same host population and, thus, suppress spread of one viruses. In this study, we performed a statistical analysis investigate temporal correlation using globally reported data 2015–2020. This was...

serovars have been isolated from both poultry and humans, can transmit Salmonella to humans (Osman et al., 2010; European Food Safety Authority [EFSA], 2019).The international trade of is one the factors affecting spread salmonellosis (EFSA, 2019) by acting as a portable reservoir for 2010), transmitting infection through food production chain (Velhner 2018), vertically infected parents (Foley 2011; Osman 2014b), transmission multidrug-resistant (MDR) in Mediterranean region (Le Hello...

Raoultella ornithinolytica is one of the emerging gram-negative bacteria, which associated with foodborne illness. Researches affirmed that distinguish between R. and Klebsiella oxytoca are difficult, as they phylogenetic related. The evolution multidrug resistance strains gained more concern for recognition pathogen supports in controlling disease minify its threat. This study sought to find a reliable tool identification ornithinolytica, isolated from chicken product samples, assessed...

In late 2016, Egypt encountered multiple cases of the highly pathogenic avian influenza (HPAI) virus H5N8 subtype. a previous study, three distinct genotypes, including A/common-coot/Egypt/CA285/2016 (H5N8) (CA285), A/duck/Egypt/SS19/2017 (SS19), and A/duck/Egypt/F446/2017 (F446), were isolated from wild birds, backyard, commercial farm, respectively, during first wave infection. this current we investigated differences in pathogenicity, replication transmissibility genotypes...

Campylobacter is one of the most important four global diarrheal diseases. It considered to be common bacterial cause human gastroenteritis in world causing a disease called <i>Campylobacteriosis</i>. In developing countries, <i>Campylobacteriosis</i> children under age 2 years are especially frequent and sometimes resulting death [1]. Mainly <i>C. jejuni</i> C. Coli well recognized causes campylobacteriososis with symptoms ranging from mild watery diarrhea serious neuropathies [2].

Total aflatoxins residues were detected in chicken and turkey tissues "muscles liver " by using HPLC. Results revealeda significant difference variation among muscles examined turkey's samples (p values <0.05).These indicated that is the reservoir place of total residues. The means average Aflatoxin B1, G1, B2,G2 respectively, 17.3+/-3.3 μg/kg, 13.5+/-2.1μg/kg,7.6+/-4.8 μg/kg ,1.5+/-0.9 22.8+/-4.1μg/kg. while they 6.5+/-1.03 μg/kg,4+/-1.4 1.7+/-0.6 0.7+/-0.3 8.9+/-1.5 μg/kg. On other side...

Ciprofloxacin residues were detected in chicken and turkey tissues "thigh, breast, liver kidney" by using HPLC.Results reflected a significant difference variation among kidney, liver, breast thigh examined turkeysamples as p values <0.05 indicating that kidney are the site of accumulation ciprofloxacin Thehighest residual level found then Whereas lowest concentrations breastthen muscles. The means average samples , breastand respectively, 316+/-26.4 μg/kg 211.1+/-20.6 μg/kg, 131.7+/-25.2...

One hundred and sixty clinically diseased broiler local chickens collected from 22 different farms belonging to Qalyubia, Dakahlia Gharbia governorates were subjected bacteriological examination molecular characterization.Clinical signs chronic respiratory disease with manifestations,lameness, loss or reduction in egg production.Bacteriological showed that 18.18% of the isolates Mycoplasma positiveand growth pleuropneumonia-like organism(PPLO) agar plates, fried appearance when examined by...

Escherichia coli infection is one of the most serious problems responsible for high losses in poultry. This paper deals with review on ceftiofur sodium and garlic showing their potential antibacterial activity . One hundred twenty apparently healthy, day old Hubbard broiler chicks were divided into 6 equal groups. Gp. (1) was kept as control. (2) supplemented 5% from till 35 days old. (3) infected via crop E. O111 (3 X                                                             10 8 CFU) at...

Hemorrhagic Gastroenteritis (HGE) is a life-threatening disease caused by bacteria or viruses endoparasites irritant drugs food allergies. Out of 202 sampled animals, the total bacterial isolates were 104. The identified 46 (44.23%) E. coli, 9 (8.65%) Klebsiella, 5 (4.80%) C. perfringens, 22 (21.15%) Proteus spp., 3 (2.88%) Salmonella Shigella spp.  and 4 (3.84%) Pseudomonas aeruginosa, Enterobacter species, 2 (1.92%) Citrobacter Providencia rettgeri, 1 (0.96%) Hafnia Serratia liquefaciens,...

Salmonellosis is an important bacterial disease usually infects poultry flocks.It caused by a wide variety of Salmonella species (Haider et al. 2004).It represents critical food safety hazard (Vinueza-Burgos 2019).Also one the most zoonotic foodborne pathogens (WHO 2016).Salmonellae infections in can be grouped into two categories; first includes non-motile serotypes, S. pullorum and gallinarum, that causes fowl typhoid, respectively (Barrow Neto 2011).The second with numerous motile as...

A total of 200 cases diseased and recently dead different poultry species (100 broiler chickens, 50 laying hens, 30ducks 20 turkeys) with the same prevalence from liver, lung heart blood were collected localities in El- Ismailia Governorate. E. coli was isolated 102(51%) cases. Only representive 10 isolates serotyped as O111:K58, O1:K1and O146:K99 order frequency (60%, 20%and 20%) isolates, respectively. Selected 13 tested for their susceptibility to antimicrobial agents absolute resistance...

Listeria monocytogenes has became increasingly important as a food-associated pathogen.It can cause rare but serious disease called listeriosis with high fatality rates (20-30%) compared other foodborne microbial pathogens.To estimate the incidence and levels of spp. in different food sources, A total 200 random samples which collected from sources 65 isolated poultry , 45 byproducts (Frozen chicken meat balls (kofta), Frozen burger frozen sausages), 20 pasteurized milk ,30 products (Feta...

This study aimed to characterize the genetic diversity, evolutionary level, and prevalence of genotypes common isolates Salmonella (Salmonella Enteritidis Typhimurium). Using one most advanced molecular recognition techniques, multilocus variable number tandem repeat analysis (MLVA), we characterized genotype S. Typhimurium.One hundred twenty-five internal organ samples were collected from major chicken slaughterhouses in Egypt, species isolated. PCR was utilized amplify IE-1 Flic-C genes...