A5063824547- Sugarcane Cultivation and Processing
- SARS-CoV-2 and COVID-19 Research
- Atrial Fibrillation Management and Outcomes
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Cardiac Arrhythmias and Treatments
- SARS-CoV-2 detection and testing
- Blood groups and transfusion
- Plant Physiology and Cultivation Studies
- Plant responses to water stress
- Hepatitis B Virus Studies
- Platelet Disorders and Treatments
- Membrane Separation and Gas Transport
- Cholesterol and Lipid Metabolism
- Viral Infections and Outbreaks Research
- Cardiac, Anesthesia and Surgical Outcomes
- Nuclear Engineering Thermal-Hydraulics
- Catalysts for Methane Reforming
- Reproductive tract infections research
- Toxoplasma gondii Research Studies
- interferon and immune responses
- Cardiac Fibrosis and Remodeling
- Cardiac pacing and defibrillation studies
- Cardiac Imaging and Diagnostics
Guangxi University
2022-2023
Lanzhou University
2021-2023
Lanzhou University Second Hospital
2021-2023
Bohai University
2021-2022
Beijing University of Chemical Technology
2017
Huazhong University of Science and Technology
2003-2005
Objective This study was aimed to investigate the risk of recurrence in patients with atrial fibrillation (AF) after radiofrequency ablation and predict using C2HEST HATCH scores. Methods We retrospectively included 322 AF from Second Hospital Lanzhou University, 261 were analysis finally. They had admitted for catheter ablation. compared ability scores AF. The predictive estimated by area under receiver operating characteristic curve (AUROC). difference between two models DeLong test....
Sugarcane somatic cell hybridization can break through the barrier of genetic incompatibility between distantly related species in traditional breeding. However, molecular mechanisms sugarcane protoplast regeneration and conditions for preparation remain largely unknown. In this study, young (ROC22) leaves were enzymatically digested, viability protoplasts reached more than 90% after enzymatic digestion (Enzymatic combination: 2% cellulase + 0.5% pectinase 0.1% dissociative enzyme 0.3%...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus which has resulted in the Disease 2019 (COVID-19) pandemic and infected millions of people all over world. SARS-CoV-2 been mutating rapidly resulting emergence multiple variants to escape host immune system mainly by mutations its receptor binding domain (RBD) spike protein. This rapid evolution posed great challenge regarding efficacy effectiveness current vaccines. The RBD full-length main target...
Fenlong-ridging (FL) is a new type of conservation tillage. In many crops, FL increases crop yield and quality; however, the cytology molecular mechanisms crops under not completely understood. This study investigated soil physical chemical properties conventional tillage (CK) during 2018–2019 (plant cane) 2019–2022 (first stubble), analyzed agronomic trait, physiology, leaf anatomical structure, gene expression related to photosynthesis between CK sugarcane (Guitang 42). Soil bulk density...
Somatic cell fusion is a process that transfers cytoplasmic and nuclear genes to create new germplasm resources. But our limited understanding of the physiological molecular mechanisms shape protoplast responses fusion.We employed flow cytometry, cytology, proteomics, gene expression analysis examine sugarcane (Saccharum spp.) fusion.Flow cytometry revealed rate protoplasts was 1.95%, FSC value SSC heterozygous cells 1.17-1.47 times higher than protoplasts. The viability decreased MDA...
The influence of low oxygen on the biosynthesis aroma-related esters and alcohols in strawberries has been well revealed. However, how low-oxygen conditions affect other volatile compounds, such as terpenes furans, is still to be elucidated.The effects 2 kPa O2 aroma 'Benihoppe' were comprehensively investigated this study. results showed that, like esters, accumulations key terpene furans also inhibited by during storage subsequent shelf life, which was associated with down-regulation...
The impact of low-oxygen (2 kPa) controlled atmosphere storage on the recovery aroma biosynthesis in ‘Golden Delicious’ (GD) apple tissues during their subsequent shelf life was investigated. results showed that highest ester content found skin tissue, followed by Flesh 2 and 1. kPa O2 GD apples resulted a decrease emission volatile esters alcohols, but an increase aldehyde emission. Notably, compared with flesh tissue O2-stored had relatively high capacity low alcohol synthesis for its...
The protoplast experimental system eis an effective tool for functional genomics and cell fusion breeding. However, the physiological molecular mechanisms of response to enzymolysis are not clear, which has become a major obstacle regeneration. Here, we used physiological, cytological, proteomics gene expression analysis compare young leaves sugarcane enzymolized protoplasts. After enzymatic digestion, obtained protoplasts with viability > 90%. Meanwhile, content malondialdehyde,...
Accurate human leucocyte antigen (HLA) and platelet (HPA) typing is essential for establishing a blood donor bank to deal with refractoriness in patients undergoing multiple transfusions. Current methods, such as Sanger next-generation sequencing, encounter difficulties haplotyping. Herein, the aim of this study was establish method HLA HPA based on long read sequencing.
The ABO system is the first and most important blood discovered in humans. It highly intricate, encompassing not only common types A, B, O, AB but also numerous variants. diversity of variants crucial for accurately defining phenotypes providing valuable information transfusions.1, 2 Here, we describe a novel variant c.505C > T based on ABO*B.01 allele Chinese individual with Bel phenotype. sample was collected from donor Taiyuan Blood Center, Shanxi, China. cell typing serum were performed...
Vasovagal reactions (VVRs) are the most common adverse and frequently associated with serious donor events. Even mild VVRs can lead to a significant reduction in likelihood of subsequent donations. The purpose this study is explore factors related occurrence after plasma donation construct nomogram identify individuals at risk for improve safety donors.We collected data from July 2019 June 2020 center Sichuan, China, independent vasovagal reactions. From these data, we constructed validated...
Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB) assays were developed quantitate nAb by monitoring ability sera inhibit spike (S) protein angiotensin converting enzyme 2 (ACE2) receptor. Herein, we established a bead-based flow cytometric CB assay and tested performance six combination models, i.e....
Using abandoned white cells separated from preparation of blood products to cultivate NK in vitro, and optimize the method cultivation allogeneic for clinical application.Abandoned production were collected 15 healthy donors. PBMCs isolated cultured 17 days using culture bottles as previously coated antibodies (group CD3 mAb was with mAb, group CD 16mAb CD16mAb, mAb+ CD16 mAb). Flow cytometry used determine ratio CD3(-)CD56(+) cells, expression activated cell surface receptors, secretion...
Abstract The protoplast experimental system has been becoming a powerful tool for functional genomics and cell fusion breeding. However, the physiology molecular mechanism during enzymolysis is not completely understood become major obstacle to regeneration. Our study used physiological, cytology, iTRAQ (Isobaric Tags Relative Absolute Quantification) -based proteomic RT-PCR analyses compare young leaves of sugarcane (ROC22) protoplasts more than 90% viability. We found that oxidation...
The effects of 2%O2 low oxygen plus 98%N2 at 0°C for 56 days following a simulated shelf life 20°C on storage quality, energy levels and aroma biosynthesis 'Benihoppe' strawberries were investigated by comparing with 21%O2 ± 79%N2 normal atmospheres. result shows that compared atmosphere, low-oxygen condition suppressed the fruit respiratory intensity lowered ATP content charge. accumulations key esters, terpene alcohols furanones also inhibited during subsequent life, which was associated...