A5058734993- interferon and immune responses
- Polyamine Metabolism and Applications
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Viral Infections and Vectors
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Biopolymer Synthesis and Applications
- Ubiquitin and proteasome pathways
- Wastewater Treatment and Nitrogen Removal
- Antimicrobial agents and applications
- Protist diversity and phylogeny
- Enzyme Structure and Function
- Cytokine Signaling Pathways and Interactions
- Neural Networks and Applications
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- Microbial Community Ecology and Physiology
- Amino Acid Enzymes and Metabolism
- Advanced Data and IoT Technologies
- SARS-CoV-2 detection and testing
- Complement system in diseases
- vaccines and immunoinformatics approaches
Roche (China)
2020-2025
Ministry of Education of the People's Republic of China
2024
Northwest University
2024
Xi'an Polytechnic University
2023-2024
Shanxi University
2021-2022
Tongji University
2022
National Institute of Allergy and Infectious Diseases
2016-2020
National Institutes of Health
2016-2020
Henan University
2019
Shanghai Jiao Tong University
2015-2016
The rise of multidrug resistant (MDR) Gram-negative (GN) pathogens and the decline available antibiotics that can effectively treat these severe infections are a major threat to modern medicine. Developing novel against MDR GN is particularly difficult as compounds have permeate double membrane, which has very different physicochemical properties, circumvent plethora resistance mechanisms such multiple efflux pumps target modifications. bacterial type II topoisomerases DNA gyrase (GyrA2B2)...
Pseudomonas aeruginosa and many other bacteria can utilize biogenic polyamines, including diaminopropane (DAP), putrescine (Put), cadaverine (Cad), spermidine (Spd), as carbon and/or nitrogen sources. Transcriptome analysis in response to exogenous Put Spd led the identification of a list genes encoding putative enzymes for catabolism polyamines. Among them, pauA1 pauA6, pauB1 pauB4, pauC, pauD1 pauD2 (polyamine utilization) encode homologous Escherichia coli PuuABCD γ-glutamylation pathway...
Malaria infection induces complex and diverse immune responses. To elucidate the mechanisms underlying host-parasite interaction, we performed a genetic screen during early (24 h) Plasmodium yoelii in mice identified large number of interacting host parasite genes/loci after transspecies expression quantitative trait locus (Ts-eQTL) analysis. We next investigated E3 ubiquitin ligase gene (March1) that was clustered with interferon (IFN)-stimulated genes (ISGs) based on similarity genome-wide...
PKMYT1 is a crucial regulator of the cell cycle, particularly involved in G2/M transition through inhibitory phosphorylation CDK1, and promising therapeutic target for cancer therapy. Data mining Roche kinome screen database identified hit characterized by 100% activity at 10 μM concentration, which was further validated with enzymatic assay showing double-digit nanomolar potency. The featured quinolinone central core phenol headgroup. replacement problematic headgroup an indazole moiety...
Abstract Advancing effective cancer therapies requires innovative approaches to better understand complex biological interactions. PKMYT1, a crucial regulator of the cell cycle involved in G2/M transition through inhibitory phosphorylation CDK1, has emerged as promising therapeutic target. Inactivation PKMYT1 is synthetically lethal tumor cells with genomic alterations leading high replication stress, such CCNE1 amplification, which frequently found high-grade serous ovarian, uterine, and...
Both type I interferon (IFN-I) and CD40 play a significant role in various infectious diseases, including malaria autoimmune disorders. is mostly known to function adaptive immunity, but previous observations of elevated levels early after infection mice led us investigate its roles innate IFN-I responses disease control. Using Plasmodium yoelii nigeriensis N67 C57BL/6 mouse model, we showed that infected CD40-/- had reduced STING serum IFN-β day-2 post infection, higher day-4 parasitemia,...
A unique D-to-L racemization of arginine by coupled dehydrogenases DauA and DauB encoded the dauBAR operon has been recently reported as a prerequisite for D-arginine utilization sole source carbon nitrogen through L-arginine catabolic pathways in P. aeruginosa. In this study, enzymic properties FAD-dependent d-amino acid dehydrogenase physiological functions were further characterized with other acids. These results establish D-amino broad substrate specificity, D-Arg D-Lys two most...
Abstract Bacterial persister cells are highly tolerant to antibiotics and cause chronic infections. However, little is known about the interaction between host immune systems with this subpopulation of metabolically inactive direct effects factors (in absence cells) on have not been studied. Here we report that human granulocyte macrophage-colony stimulating factor (GM-CSF) can sensitize Pseudomonas aeruginosa PAO1 PDO300 multiple including ciprofloxacin, tobramycin, tetracycline gentamicin....
Malaria is a deadly parasitic disease that continues to afflict hundreds of millions people every year. Infections with malaria parasites can be asymptomatic, mild symptoms, or fatal, depending on delicate balance host immune responses. enter red blood cells (RBCs) through interactions between parasite ligands and receptors, such as erythrocyte-binding-like (EBL) proteins Duffy antigen receptor for chemokines (DARC). Plasmodium yoelii EBL (PyEBL) known play role in invasion RBCs. Here, we...
ABSTRACT Exogenous spermine was reported to enhance the killing of methicillin-resistant Staphylococcus aureus (MRSA) by β-lactams through a strong synergistic effect unknown nature. Spermine alone also exerts an antimicrobial activity against S. in pH-dependent manner. MIC measurements revealed stronger effects under alkaline conditions, suggesting nucleophilic property instead its positive charge as cause adverse effects. A spontaneous suppressor mutant (MuM) MRSA Mu50 selected for...
Monocyte chemoattractant protein-1 (MCP-1) plays a crucial role in various inflammatory diseases. To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents, we establish transgenic mouse line. The firefly luciferase gene is incorporated into genome driven by endogenous promoter. A bioluminescence photographing system applied monitor levels live mice inflammation, including lipopolysaccharide-induced sepsis, concanavalin A-induced T cell-dependent liver injury, CCl...
ABSTRACT Polyamines are absolute requirements for cell growth. When in excess, Pseudomonas aeruginosa possesses six γ-glutamylpolyamine synthetases (GPSs) encoded by the pauA1-pauA7 genes to initiate polyamine catabolism. Recently, pauA2 mutant was reported lose capability grow on spermine (Spm) and spermidine (Spd) as sole carbon nitrogen sources. Although this grew normally defined minimal medium LB broth, growth completely abolished addition of Spm or Spd. These two compounds exert a...
Seven imipenem-resistant Pseudomonas aeruginosa isolates were recovered from the sputum samples of pneumonia patients in southwestern China. They had identical antibiotic resistance patterns and indistinguishable pulsed-field gel electrophoresis profiles. Nucleotide sequence analysis revealed a 4-bp (AGTC) insertion oprD gene, resulting frameshift cognate open reading frame. These became imipenem susceptible when chromosomal lesion was complemented, indicating that gene resulted resistance.
Optimization of multilayer restoration and routing was investigated. Compared to TD, BU has higher probability much shorter time. EROTRIP proposed guarantee high for traffic with priority.
The draft genome sequence of Thauera sp. DTG was reconstructed from a metagenome denitrifying quinoline degrading microbial consortium. organism is most closely related to aminoaromatic S2 and MZ1T facultative anaerobe. It predicted take the central role for degradation.
Currently, assays for rapid therapeutic drug monitoring (TDM) of β-lactam antibiotics in blood, which might be benefit optimizing doses treatment critically ill patients, remain challenging. Previously, we developed an assay determining the penicillin-class blood using a thermometric penicillinase biosensor. The eliminates sample pretreatment, makes it possible to perform semicontinuous penicillin determinations blood. However, has narrow substrate specificity, unsuitable detecting other...
Accurate detection of SARS-CoV-2 neutralizing antibody (nAb) is critical for assessing the immunity levels after virus infection or vaccination. As fast, cost-effective alternatives to viral infection-based assays, competitive binding (CB) assays were developed quantitate nAb by monitoring ability sera inhibit spike (S) protein angiotensin converting enzyme 2 (ACE2) receptor. Herein, we established a bead-based flow cytometric CB assay and tested performance six combination models, i.e....
The draft genome sequence of Thauera sp. DTG was reconstructed from a metagenome denitrifying quinoline degrading microbial consortium. organism is most closely related to aminoaromatic S2 and MZ1T facultative anaerobe. It predicted take the central role for degradation.
Abstract Enzymatic conversion is an important method for producing rare ginsenosides. In order to explore the molecular mechanisms underlying interaction of β-Glucanase during process ginsenoside CK, this study employed high-performance liquid chromatography, infrared spectroscopy, ultraviolet and fluorescence spectroscopy investigate ability convert Rb1 into CK. Changes in secondary structure quantity enzyme before after were characterized. Additionally, accurated binding sites with Rb1, as...
Abstract In order to maintain the dynamic physiological balance, plants are compelled adjust their energy metabolism and signal transduction cope with abiotic stresses caused by complex changeable environments. The diterpenoid natural compound secondary metabolites, sclareol, derived from Salvia sclarea , has gained significant attention owing its economic value as a spice material diverse activities. Here, we focused on roles regulatory mechanisms of sclareol diterpene synthase gene SsdTPS...
Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly emerging bacteria causing infection, which has developed resistance to most of the beta-lactam antibiotics because newly acquired low-affinity penicillin binding protein (PBP2a), can continue build cell wall when other PBPs are blocked by beta-lactams. Exogenous spermine exerts dose dependent inhibition effect on growth E. coli, Salmonella enterica serovar and aureus. We have selected an MRSA Mu50 derivative harbors mutation...