Arf GTPase-activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors. ArfGAPs are critical for cargo sorting in Golgi-to-ER traffic. However, role into intralumenal vesicles (ILVs) multivesicular bodies (MVBs) post-Golgi traffic remains unclear. Exosomes extracellular (EVs) endosomal origin. CD63 is an EV marker. enriched ILVs MVBs cells. secretion positive EVs has not been consistent with data on localization MVBs, and how CD63-containing formed yet be...

Since increased cerebral oxygenation reflects activation, this study investigated the effect of mastication frequency on prefrontal cortex oxygenation. Eleven young volunteers (nine women, two men; M age = 20.9 years, SD 0.9) carried out three trials in which they were asked to chew a tasteless gum for 3 min at varying (rates frequency: 30, 70, and 110). Breaks 2 each interleaved between trials. The left was monitored by near-infrared spectroscopy. We found significant increase cortical...

The nucleotide oligomerization domain (NOD)-like receptor 2 (NOD2) is an intracellular pattern recognition that detects components of peptidoglycans from bacterial cell walls. NOD2 regulates bowel microorganisms, provides resistance against infections such as diarrhea, and reduces the risk inflammatory diseases in humans mice. We previously demonstrated a specific porcine polymorphism (NOD2-2197A > C) augments peptidoglycan components. In this study, relationships between NOD2-2197A/C...

Abstract To clarify the persistence of extended‐spectrum β‐lactamase (ESBL) producers, 13 plasmids from two broiler farms were analyzed. On farm not using antimicrobials, one plasmid Klebsiella pneumoniae isolated a day‐old chick was similar to that Escherichia coli year later, with deletion transposons. most circulating (eight out nine) in flock 40‐days‐old chicks identical, although K. had transposon carrying class 1 integron containing aadA2 and dfrA12 . Thus, ESBL persisted or without...

The increase in antimicrobial-resistant (AMR) bacteria caused by antimicrobial usage is a public health problem. We investigated the proportion of cephalexin (LEX)-resistant fresh feces obtained from antimicrobial-free broilers three flocks at <15, 15–40, and> 40 days old. DHL agar plates containing 25 μg/mL LEX (DHL-L) showed LEX-resistant all <15 old and one flock > bacterial counts on DHL-L were 105–108 colony forming units (CFU)/g <102–105 CFU/g feces, respectively. also assessed AMR...

Abstract Arf GTPase-Activating proteins (ArfGAPs) mediate the hydrolysis of GTP bound to ADP-ribosylation factors. ArfGAPs are critical for cargo sorting in Golgi-to-ER traffic. However, role into intralumenal vesicles (ILVs) multivesicular bodies (MVBs) post -Golgi traffic remains unclear. Exosomes extracellular (EVs) endosomal origin. EVs cell-to-cell communication, and CD63 is an EV marker. enriched MVBs cells. secretion positive has not been consistent with data on localization MVBs, how...

Introduction: Osimertinib (AZD9291) is a third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor that has shown significant clinical benefits in patients with EGFR-sensitizing mutations or the EGFR T790M mutation. The homologous recombination (HR) pathway crucial for repairing DNA double-strand breaks (DSBs). Rad51 plays central role HR, facilitating search homology and promoting strand exchange between molecules. overexpressed numerous types of cancer cells. B02,...

We previously showed that several polymorphisms in genes encoding pattern recognition receptors cause amino acid substitutions alter pathogen ability and disease susceptibility pigs. In this study, we expanded our analysis to a wide range of immune-related investigated polymorphism distribution its influence on pneumonia multiple commercial pig populations. Among the 42 causing 634 extracted from swine genome database, 80 24 were found have minor allele frequency at least 10% Japanese...

Reduced productivity caused by infections, particularly respiratory diseases, is a serious problem in pig farming. We have previously reported polymorphisms porcine pattern recognition receptor genes affecting molecular functions and demonstrated that the 2197A/C polymorphism nucleotide-binding oligomerization domain containing 2 (NOD2) gene influences circovirus 2-induced mortality. Here, we investigated how these affect disease-induced lesions, using samples from slaughterhouse dealing...

In recent years, there has been an increase in the number of hypertensive diseases and various associated with them. A major cause these is excessive salt intake. The purpose present study was to examine whether chewing hard foods lowers saltiness threshold. Fifteen subjects (fourteen women one man) participated study. Two types gummies are available as ingredients: soft gummies. thresholds before after each gummi were studied using 11 different NaCl solutions. Then, points subjective...

Emm抗原は,国際輸血学会(ISBT)において高頻度抗原(901008)に認定されている.これまで海外で8例の抗Emm保有例が報告されているが,抗体保有者に輸血が行われた報告例はなく,自然発生による抗体の可能性が高い.また臨床的意義は不明である.今回,58歳男性患者が緊急に輸血が必要となり,輸血前の検査において抗Leaと高頻度抗原に対する抗体の保有が疑われたが,やむなく交差適合試験不適合のLe(a-)型の赤血球濃厚液-LR(以下RCC)2単位の輸血を行ったところ,輸血終了30分後に血圧低下および血色素尿が出現した.さらに同日RCC 4単位,3日目に2単位を輸血したが,この時は明らかな副作用を認めなかった.しかし,6日目にRCC 2単位の輸血を行ったところ,30ml使用した時点で,嘔吐,赤褐色尿を認め輸血中止となった.精査の結果,抗Emmと同定され,本邦での検出は第1例目となる.本症例はこれまでの報告と同様に輸血歴が確認されておらず,抗Emmは自然抗体の可能性が高く,新たに重篤な急性溶血性輸血副作用の原因となる臨床的に意義のある抗体の可能性が示唆された.

The purpose of this study was to investigate whether the opinions people eating together affect taste foods they eat. In addition, if others influence for one with them, are others’ related empathy individuals? Finally, also intended consider threshold changes depending on others. Twelve healthy young women (aged 18.4 ± 0.8 years; mean SD) participated in present study. participants tasted bread under three conditions: 1) quiet condition: a participant ate persons, 2) positive persons who...

In the present study, we examined cognitive function during mastication of lemon-flavored gum, which is said to enhance cerebral blood flow. Nine healthy subjects (8 female and 1 male) participated in this study. Subjects chewed gum for 3 min after fasting 2 h conducted a Stroop test while continuing chew. At end all answers, chewing ended. The response time was used as an indicator function. We set three conditions (lemon-flavored mint-flavored no chewing). There significant difference...

Swine Mycoplasma Hyopneumoniea, hp, is known as a major factor to affect for the specific pneumonia (MPS). This damages very serious because carrier rate of hp in piglets from 3 4 months age high, that response antibody shows positive 80 % over, and has terrible tissue MPS 51% Japanese pig farm. We bred resistant strain by selection decrease pathogenic condition over 5 generations using Landrase (MPS strain), high immune performance peripheral phagocytosis, complement activity production...

While people might endorse tolerance, social/environmental biases can remain operative and drive action in an unconscious manner. Herein, we investigated whether the criteria for judging others’ body shapes as “fat” or “thin” change with degree of satiety. Nine females participated present study. The participants judged nine women’s figures fat skinny on a computer monitor two conditions (Fasting Satiety). Each figure ranged mass index (BMI) from 18.3 to 45.4 (i.e., 18.3, 19.3, 20.9, 23.1,...

Communal eating reportedly induces changes in food-related behaviors such as increased consumption and alleviation of indefinite complaints. Here, we examined the influence intimacy with co-eaters on palatability food physiological taste thresholds. The study was a single-blind crossover trial 16 healthy women aged 18 - 19 years (two close friends × 8) participants. We effect four preset conditions regard to (condition 1 = tasting alone; condition 2 friend; 3 three unfamiliar individuals; 4...

精米もしくは精米と米ぬかの併用給与が豚肉質に及ぼす影響を検討した．供試豚は，平均体重70kgのデュロック種雌豚32頭を4つの区に割り振った．区分は，トウモロコシを70%配合した対照区，対照区のトウモロコシを精米に代替した精米区，精米を60%と米ぬかを10%配合した精米+米ぬか10%区および精米50%と米ぬかを20%配合した精米+米ぬか20%区とした．精米区の背脂肪内層L*値とオレイン酸割合は，対照区よりも高く（P<0.05），米ぬかの配合割合が増すと対照区との差が無くなった．精米区の背脂肪内層リノール酸とα-リノレン酸割合は，対照区に比べて低く（P<0.05），米ぬかの配合割合が増すと対照区と同程度まで増加した．以上より，精米給与は，背脂肪内層のL*値とオレイン酸割合を増加させ，多価不飽和脂肪酸酸割合を低下させた．米ぬかの配合割合の増加は，この効果を減少させることが明らかになった．