A5070184745- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Traditional Chinese Medicine Analysis
- Molecular Sensors and Ion Detection
- Natural product bioactivities and synthesis
- Supramolecular Self-Assembly in Materials
- Mycotoxins in Agriculture and Food
- Luminescence and Fluorescent Materials
- Chromatography in Natural Products
- Phytochemistry and Biological Activities
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Chemical Synthesis and Analysis
- Polyamine Metabolism and Applications
- DNA Repair Mechanisms
- Analytical Chemistry and Chromatography
- Pharmacological Effects of Natural Compounds
- Erythrocyte Function and Pathophysiology
- Supramolecular Chemistry and Complexes
- Peptidase Inhibition and Analysis
- Synthesis of Organic Compounds
- Kruppel-like factors research
- Hippo pathway signaling and YAP/TAZ
Tianjin University of Traditional Chinese Medicine
2018-2025
Guangdong Pharmaceutical University
2024-2025
Tianjin haihe hospital
2023-2025
University of Science and Technology of China
2008-2024
Nankai University
2023
Hefei National Center for Physical Sciences at Nanoscale
2008-2020
Center for Excellence in Molecular Cell Science
2013-2019
University of Occupational and Environmental Health Japan
2011-2013
Tohoku University
2013
Morehouse School of Medicine
2013
Perfluorinated alkyl substances, such as perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), are toxic materials that known to globally contaminate water, air, soil resources. Strategies for the simultaneous detection removal of these compounds desired address this emerging health environmental issue. Herein, we develop a type guanidinocalix[5]arene can selectively strongly bind PFOS PFOA, which use demonstrate sensitive quantitative in contaminated water through fluorescent...
Abstract Characterized by an excessively increased uric acid (UA) level in serum, hyperuricemia induces gout and also poses a great threat to renal cardiovascular systems. It is urgent meaningful perform early warning noninvasive diagnosis, thus conducing blockage of disease aggravation. Here, guanidinocalix[5]arene (GC5A) successfully identified from the self‐built macrocyclic library specifically monitor UA urine indicator displacement assay. strongly bound GC5A at micromolar‐level, while...
SETD3 is a member of the SET (Su(var)3–9, Enhancer zeste, and Trithorax) domain protein superfamily plays important roles in hypoxic pulmonary hypertension, muscle differentiation, carcinogenesis. Previously, we identified as actin-specific methyltransferase that methylates N3 His73 on β-actin (Kwiatkowski et al., 2018). Here, present two structures S-adenosyl-L-homocysteine-bound complex with either an unmodified peptide or its His-methylated variant. Structural analyses, supported by...
Detection and quantification of trimethylamine
Abstract The generation of cultured red blood cells (cRBCs) ex vivo represents a potentially unlimited source for RBC transfusion and other cell therapies. Human cRBCs can be generated from the terminal differentiation proliferating erythroblasts derived hematopoietic stem/progenitor or erythroid precursors in peripheral mononuclear cells. Efficient maturation into highly depend on replenishing human plasma, which exhibits variable potency across donors batches complicates consistent cRBC...
Entosis, a cell-in-cell process, has been implicated in the formation of aneuploidy associated with an aberrant cell division control. Microtubule plus-end-tracking protein TIP150 facilitates loading MCAK onto microtubule plus ends and orchestrates plus-end dynamics during division. Here we show that cooperates to govern entosis via regulatory circuitry involves Aurora A-mediated phosphorylation MCAK. Our biochemical analyses forms intra-molecular association, which is essential for binding....
Screening inhibitors of flavin monooxygenase 3 (FMO3) is very important for treating trimethylamine N-oxide (TMAO) derived thrombotic diseases. Herein, focusing on Xuefu Zhuyu decoction (XFZYD) as a Chinese traditional medicine with antithrombotic efficacy, facile and label-free fluorescence strategy was developed evaluating the influence bioactive ingredients in XFZYD FMO3 activity through indicator displacement assay. To this end, optimized supramolecular host-guest...
To ensure food safety and quality, sensitive accurate methods for rapidly detecting mycotoxins have become imperative. Zeranols (ZERs) are a class of commonly found in cereals, posing serious health risks, including hormonal disruption carcinogenic potential. In response to this pressing concern, we developed simple yet highly high-throughput supramolecular sensing approach based on novel macrocycles known as extended biphen[n]arenes monitoring ZERs cereal matrices. The proposed utilizes the...
The spindle assembly checkpoint (SAC) is a surveillance mechanism monitoring cell cycle progression, thus ensuring accurate chromosome segregation. conserved mitotic kinase Mps1 key component of the SAC. human exhibits comprehensive phosphorylation during mitosis. However, related biological relevance largely unknown. Here, we demonstrate that 8 autophosphorylation sites within N-terminus Mps1, outside catalytic domain, are involved in regulating kinetochore localization. phospho-mimicking...
To achieve reliable, effective, and rapid quality evaluation of complex traditional Chinese medicine (TCM) products, we proposed a supramolecular fluorescence sensing method based on pattern recognition. An azocalixarene-based sensor array was constructed to evaluate batch-to-batch consistency Qufeng Zhitong Capsule (QZC). The molecular recognitions between azocalixarenes the contents QZC are transduced through signal output itself or applied indicator. Data simulations show that differences...
The digestive function of the stomach depends on acidification gastric lumen. Acid secretion into lumen is triggered by activation a cAMP-dependent protein kinase (PKA) cascade, which ultimately results in insertion H,K-ATPases apical plasma membranes parietal cells. A coupling ezrin whose phosphorylation at Ser-66 PKA required for cell activation. However, little known regarding molecular mechanism(s) operates acid secretion. Here we show that induces conformational change enables its...
In the face of diversified analytes, it is a great challenge and infeasible task to design synthesize corresponding macrocyclic hosts realize ideal supramolecular sensing. Herein, we proposed novel sensing strategy, guest adaptative assay (GAA), in which analyte was quantitatively transformed under mild conditions perfectly adapt host. As health-threatening "landmine" cereals, aflatoxins were converted by aid alkali hydrolysis satisfactorily obtain transformants ionic state, resulting...
We designed a hyaluronidase/ATP tandem stimuli-responsive assembly based on guanidinium-modified calix[5]arene and eosin Y modified hyaluronic acid.
The root of Polygala tenuifolia Willd. or sibirica L. exhibits protective effects on the central nervous system and is frequently used to treat insomnia, amnesia, other cognitive dysfunction. In our study, we studied nine bioactive compounds spanning oligosaccharide esters, saponins, xanthones by using a sensitive, efficient, validated method established ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry. quantified result interesting proved that...
Post-translational modifications play important roles in mediating protein functions a wide variety of cellular events vivo. HEMK2-TRMT112 heterodimer has been reported to be responsible for both histone lysine methylation and eukaryotic release factor 1 (eRF1) glutamine methylation. However, how complex recognizes catalyzes eRF1 is largely unknown. Here, we present two structures HEMK2-TRMT112, with one bound SAM the other SAH methylglutamine (Qme). Structural analyses post-catalytic...
As a traditional Chinese medicine, Eucommia ulmoides Oliver (E. Oliv.) is an important medicinal plant, and its barks, male flowers, leaves, fruits have high value of utilization. The seed meal E. Oliv. the waste residue produced after oil extraction from seeds Though ideal feed additive, far being developed utilized. We identified six natural iridoid compounds Oliv., namely geniposidic acid (GPA), scyphiphin D (SD), ulmoidoside A (UA), B (UB), C (UC), (UD). Six were validated to...