A5004698498- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Diabetes and associated disorders
- Diabetes Treatment and Management
- Cancer-related molecular mechanisms research
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Diet, Metabolism, and Disease
- Dietary Effects on Health
- Pregnancy and preeclampsia studies
- Peroxisome Proliferator-Activated Receptors
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- FOXO transcription factor regulation
- Lipid metabolism and biosynthesis
- Circadian rhythm and melatonin
- Liver Disease Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Heat shock proteins research
Helmholtz Zentrum München
2014-2023
Deutsches Diabetes-Zentrum e.V.
2014-2022
German Center for Diabetes Research
2014-2022
Heinrich Heine University Düsseldorf
2014-2022
Pennington Biomedical Research Center
2008-2016
University of Waterloo
2010-2012
Emmanuel Bible College
2012
Center for Beta Cell Therapy in Diabetes
2005-2007
Vrije Universiteit Brussel
2000-2005
University of Lausanne
1996
Glucose homeostasis is controlled by a glucose sensor in pancreatic beta-cells. Studies on rodent beta-cells have suggested role for GLUT2 and glucokinase this control function mechanisms leading to diabetes. Little direct evidence exists so far implicate these two proteins recognition human The present vitro study investigates the of transport phosphorylation beta-cell preparations from nondiabetic pancreata. Human differ transporter gene expression (predominantly GLUT1 instead GLUT2),...
Rat pancreatic α- and β-cells are critically dependent on hormonal signals generating cyclic AMP (cAMP) as a synergistic messenger for nutrient-induced hormone release. Several peptides of the glucagon-secretin family have been proposed physiological ligands cAMP production in β-cells, but their relative importance islet function is still unknown. The present study shows expression at RNA level receptors glucagon, glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide...
Dietary methionine restriction (MR) is a mimetic of chronic dietary (DR) in the sense that MR increases rodent longevity, but without food restriction. We report here also persistently total energy expenditure (EE) and limits fat deposition despite increasing weight-specific consumption. In Fischer 344 (F344) rats consuming control or diets for 3, 9, 20 mo, mean EE was 1.5-fold higher vs. rats, primarily due to during night at all ages. The day-to-night transition produced twofold heat...
Peroxisome proliferator-activated receptor gamma co-activator-1alpha (PGC-1alpha) plays a central role in the regulation of cellular energy metabolism and metabolic adaptation to environmental nutritional stimuli. We recently described novel, biologically active splice variant PGC-1alpha (NT-PGC-1alpha, amino acids 1-270) that retains ability interact with transactivate nuclear hormone receptors through its N-terminal transactivation domain. Whereas is an unstable protein sensitive...
Abstract The number of pregnancies complicated by gestational diabetes (GDM) is increasing worldwide. To identify novel characteristics GDM, we studied miRNA profiles maternal and fetal whole blood cells (WBCs) from GDM normal glucose tolerant (NGT) pregnant women matched for body mass index age. After adjustment weight gain pregnancy week, identified 29 mature micro-RNAs (miRNAs) up-regulated in one which, i.e., miRNA-340, was validated qPCR. mRNA protein expression PAIP1, a miRNA-340...
Bezafibrate (BEZ), a pan activator of peroxisome proliferator–activated receptors (PPARs), has been generally used to treat hyperlipidemia for decades. Clinical trials with type 2 diabetes patients indicated that BEZ also beneficial effects on glucose metabolism, although the underlying mechanisms these remain elusive. Even less is known about potential role in treating 1 diabetes. Here we show markedly improves hyperglycemia and insulin tolerance mice streptozotocin (STZ)-induced diabetes,...
Combined use of metformin and a sodium glucose cotransporter 2 inhibitor (SGLT2I) is promising treatment strategy for type diabetes. The mechanism by which combination provides better glycemic control than or SGLT2I monotherapy remains elusive. Therefore, we investigated the physiological both compounds lower blood concentrations in diabetic mice. We compared potential AVE2268 alone to mitigate hyperglycemia modulate fluxes db/db Tallyho/JngJ elicited rapid decline circulating levels,...
Better disease management can be achieved with earlier detection through robust, sensitive, and easily accessible biomarkers. The aim of the current study was to identify novel epigenetic biomarkers determining risk type 2 diabetes (T2D). Livers 10-week-old female New Zealand Obese (NZO) mice, slightly differing in their degree hyperglycemia liver fat content thereby susceptibility were used for expression methylation profiling. We screened differences hepatic DNA diabetes-prone -resistant...
The metabolic pathways that are involved in regulating insulin secretion from pancreatic β-cells still incompletely understood. One potential regulator of the phenotype is transcription factor aryl hydrocarbon receptor nuclear translocator (ARNT)/hypoxia-inducible (HIF)-1β. ARNT/HIF-1β levels profoundly reduced islets obtained type 2 diabetic patients. However, no study to date has investigated key release lack ARNT/HIF-1β. In this study, we confirm siRNA-mediated knockdown inhibits...
The β3-adrenergic receptor (AR) signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. β3-AR highly induces the expression transcriptional coactivator PGC-1α its splice variant N-terminal (NT)-PGC-1α, which turn activate transcription program by co-activating number factors. We previously reported that NT-PGC-1α able to increase mitochondrial activity cultured adipocytes promoting thermogenic genes. In present study, we...
Physical training improves insulin sensitivity and can prevent type 2 diabetes (T2D). However, approximately 20% of individuals lack a beneficial outcome in glycemic control. TGF-β, identified as possible upstream regulator involved this low response, is also potent microRNAs (miRNAs). The aim study was to elucidate the potential impact TGF-β-driven miRNAs on individual exercise response. Non-targeted long sncRNA sequencing analyses TGF-β1-treated human skeletal muscle cells corroborated...
Despite their fundamental role in various biological processes, the analysis of small RNA sequencing data remains a challenging task. Major obstacles arise when short sequences map to multiple locations genome, align regions that are not annotated or underwent post-transcriptional changes which hamper accurate mapping. In order tackle these issues, we present novel profiling strategy circumvents need for read mapping reference genome by utilizing actual determine expression intensities....