A5057482995- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Parkinson's Disease Mechanisms and Treatments
- CRISPR and Genetic Engineering
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
- Alzheimer's disease research and treatments
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Estrogen and related hormone effects
- Hops Chemistry and Applications
- RNA regulation and disease
- 3D Printing in Biomedical Research
- Cholinesterase and Neurodegenerative Diseases
- Clusterin in disease pathology
- Ion channel regulation and function
- Phytochemistry and Bioactive Compounds
- Neurological diseases and metabolism
- Nuclear Receptors and Signaling
- RNA Research and Splicing
- Neuropeptides and Animal Physiology
- Genetics and Neurodevelopmental Disorders
- Nerve injury and regeneration
University of Brescia
2019-2025
Centro San Giovanni di Dio Fatebenefratelli
2023-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Cytoplasmic FMRP Interacting Protein 2 (CYFIP2) a component of the Wave Regulatory Complex (WRC), one most important players in regulating cellular actin dynamics. Interestingly, CYFIP2 transcript undergoes RNA editing, an epitranscriptomic modification catalysed by ADAR enzymes, that leads adenosine (A) to inosine (I) deamination. editing coding sequence results K/E substitution at amino acid 320. The functional meaning this regulation is still unknown. In study, we aim investigating...
Abstract The progressive neuropathological damage seen in Parkinson's disease (PD) is thought to be related the spreading of aggregated forms α‐synuclein. Clearance extracellular α‐synuclein released by degenerating neurons may therefore a key mechanism control concentration space. Several molecular chaperones misfolded protein accumulation compartment. Among these, clusterin, glycoprotein associated with Alzheimer's disease, binds species and present Lewy bodies, intraneuronal aggregates...
Chronic neuroinflammation plays a crucial role in the progression of several neurodegenerative diseases (NDDs), including Parkinson’s disease (PD) and Alzheimer’s (AD). Intriguingly, last decade, leucine-rich repeat kinase-2 (LRRK2), gene mutated familial sporadic PD, was revealed as key mediator neuroinflammation. Therefore, anti-inflammatory properties LRRK2 inhibitors have started to be considered disease-modifying treatment for PD; however, date, there is little evidence on beneficial...
Dopaminergic neurons express a heteromer composed of the dopamine D3 receptor and α4β2 nicotinic acetylcholine receptor, D3R-nAChR heteromer, activated by both nicotine D2 receptors agonists, such as quinpirole, crucial for dopaminergic neuron homeostasis. We now report that activity is potentiated 17-β-estradiol which acts positive allosteric modulator binding specific domain on α4 subunit protomer. In mouse neurons, in fact, significantly increased ability quinpirole promoting dendritic...
Abstract We have previously shown that the heteromer composed by dopamine D3 receptor (D3R) and nicotinic acetylcholine (nAChR) (D3R-nAChR heteromer) is expressed in dopaminergic neurons, activated nicotine represents molecular unit that, these contributes to modulation of critical events such as structural plasticity neuroprotection. now extended this study investigating D3R-nAChR properties using various cell models transfected HEK293 cells, primary cultures mouse neurons human derived...
Our recent study revealed that fluorescent lamp light can penetrate deep into the brain of mice and rats leading to development typical histological characteristics associated with Parkinson's disease such as loss dopamine neurons in substantia nigra. Monochromatic LED lights were thus used this work deepen our knowledge on effects major wavelength peaks mouse human dopaminergic cells. In particular, we exposed immortalized MN9D neuronal cells, primary cultures mesencephalic cells...
The heteromer composed of dopamine D1 and D3 receptors (D1R–D3R) has been defined as a structure able to trigger Erk1/2 Akt signaling in G protein-independent, beta-arrestin 1-dependent way that is physiologically expressed the ventral striatum likely involved control locomotor activity. Indeed, abnormal levels D1R-D3R dorsal have correlated with development L-DOPA-induced dyskinesia (LID) Parkinson’s disease patients, motor complication associated striatal D1R signaling, thus requiring Gs...
Peripheral blood mononuclear cells (PBMCs) derived from a healthy 40-year-old female were successfully transformed into induced pluripotent stem (iPSCs) by using the integration-free CytoTune-iPS Sendai Reprogramming method. The resulting iPSCs line exhibits normal karyotype, expresses stemness markers and displays differentiation capacity three germ layers. This human can be used as control in disease modelling studies.
Leucine-rich repeat kinase-2 (LRRK2), a gene mutated in familial and sporadic Parkinson’s disease (PD), controls multiple cellular processes important for GLIA physiology. Interestingly, emerging studies report that LRRK2 is highly expressed oligodendrocyte precursor cells (OPCs) compared to the pathophysiology of other brain oligodendrocytes (OLs) PD. Altogether, these observations suggest crucial function(s) OPCs/Ols, which would be interesting explore. In this study, we investigated role...
Novel and complementary experimental models are required for investigating the molecular mechanisms underlying resistance to available therapies of patients with major depression (Treatment-Resistant Depression, TRD) that occurs in at least one third need be deeply investigated. Here, we have established a patient-specific disease model TRD by reprogramming peripheral blood mononuclear cells (PBMCs) from two into induced pluripotent stem (iPSCs), using non-integrating Sendai virus. These...