<i>Background:</i> Tree nut allergy, a major group of food is often linked to fatal or near-fatal systemic anaphylaxis. Currently, an adjuvant-free mouse model study tree hypersensitivity unavailable. Here we tested the hypothesis that transdermal exposure hazelnut, nut, without use adjuvant sufficient sensitize mice for immediate reaction oral hazelnut challenge. <i>Methods:</i> BALB/c were repeatedly exposed protein via route and allergic anaphylactic responses...

Food allergies are potentially fatal immune-mediated disorders that growing globally. The relationship between sex and food allergy remains incompletely understood. Here we tested the hypothesis that, should influence clinical response to allergens, this would be reflected by a disparity in published studies of allergy. We performed systematic search PubMed literature for IgE-mediated 11 allergenic foods international regulatory importance. No date restriction was used only articles English...

CXCR3 binding chemokine CXCL10 (IP-10) markedly enhances antigen-specific Th1 recall responses in healthy humans, suggesting a role for this pathway maintenance of clinical tolerance to environmental allergens as well potential therapeutic ligands re-balancing the Th2-dominated that underlie generation and allergic disorders. Here, we investigated capacity modulate allergen-driven IFNgamma production by individuals characterized Th2 immunity-dominated allergen specific responses,...

<i>Background:</i> Allergy to sesame seeds is an emerging food allergy of a serious nature due high risk systemic anaphylaxis. Although mouse model study anaphylaxis desirable, currently it not available. Here, using transdermal exposure system, we tested the hypothesis that seed elicits IL-4-associated IgE antibody response with consequent clinical sensitization in mice. <i>Methods:</i> Groups BALB/c mice were exposed extract or saline control (vanilla bean extract)...

<i>Background:</i> Cashew nut allergy is an emerging food with a high risk of systemic anaphylaxis. Currently, adjuvant-free animal model to study cashew not available. <i>Methods:</i> BALB/c mice were exposed protein using transdermal sensitization protocol that does use adjuvant. Systemic IgE antibody response, anaphylaxis oral challenge and allergen-driven, spleen-cell, type-2 cytokine responses studied. <i>Results:</i> Transdermal exposure resulted in...

Abstract Wheat allergy is a potentiallylife‐threatening disease that affects millions of people around the world. Food processing has been shown to influence allergenicity wheat and other major foods. However, comprehensive review evaluating whether or not food can be used develop hypo‐/nonallergenic products unavailable. There were three objectives for this study: (1) critically evaluate evidence on effect fermentation, thermal processing, enzyme acid hydrolysis so as identify potential...

Clinically it is recognized that tree nut allergies such as hazelnut allergy are not usually outgrown. Specific mechanisms underlying the persistence of food incompletely understood. Here we studied natural history and long-term immune clinical characteristics in an adjuvant-free mouse model.BALB/c mice were sensitized to protein using a transdermal sensitization protocol does use adjuvant. After establishing sensitization, exposure was withdrawn for 3, 5 or 8 months. The fate circulating...

Wheat allergy and other immune-mediated disorders triggered by wheat proteins are growing at an alarming rate for reasons not well understood. A mouse model to study hypersensitivity responses salt-soluble protein (SSWP) extract is currently unavailable. Here we tested the hypothesis that SSWP from will induce sensitization as allergic disease in mice.Female BALB/cJ mice were weaned onto a plant protein-free diet. The injected total of 4 times with (0.01 mg/mouse) fraction extracted durum...

Wheat is a major food allergen per the regulatory bodies of various nations. Hypersensitivity reactions to wheat have been steadily increasing for reasons that are not completely understood. Wheat-allergy models typically use adjuvants induce sensitization proteins followed by an intraperitoneal challenge elicit anaphylaxis. Although these very useful, they lack ability reveal intrinsic allergenicity potential wheat. To improve mouse model allergy, we tested hypothesis repeated skin...

Wheat allergy is a major type of food with the potential for life-threatening anaphylactic reactions. Common wheat, Triticum aestivum (hexaploid, AABBDD genome), was developed using tetraploid wheat (AABB genome) and ancient diploid progenitor (DD genome)-Aegilops tauschii. The allergenicity gluten from unknown. In this study, novel adjuvant-free mouse model, we tested hypothesis that glutenin extract will be intrinsically allergenic in model. grown, berries were used to testing. A plant...

<i>Background:</i> Hazelnut is one of the major tree nuts that causes potentially fatal food allergy, with underlying mechanisms are unclear at present. One suggestion hazelnut allergy results from immune crossreactivity IgE antibodies produced against certain aeroallergens. We tested hypothesis intrinsically capable eliciting an allergic response using a mouse model. <i>Methods:</i> Groups mice were injected intraperitoneally hazelnut/filbert protein extract or...

Wheat allergies are potentially life-threatening and, therefore, have become a major health concern at the global level. It is largely unknown present whether genetic variation in allergenicity potential exists among hexaploid, tetraploid and diploid wheat species. Such information critical establishing baseline map to inform breeding efforts identify hyper-, hypo- non-allergenic varieties. We recently reported novel mouse model of intrinsic using salt-soluble protein extract (SSPE) from...

Gluten allergy is a major public health problem that growing at an alarming rate. Specific mechanisms underlying sensitization to gluten remain incompletely understood. Currently, it unclear whether chronic exposure alcohol-soluble extract via undamaged skin has the capacity clinically sensitize mice for life-threatening anaphylaxis. Using adjuvant-free mouse model, here we tested hypothesis application of durum (ASDG) will anaphylaxis.This study was conducted in gluten-free Balb/c colony...

Currently, there is neither a published ELISA method nor it clear whether chemiluminescence substrates would provide better sensitivity vs. colorimetric for measuring human IL-23-a recently described Type-1 immunity associated cytokine. Initially, we optimized using p-nitro-phenyl phosphate substrate. Subsequently, compared with that provided approximately 5-fold enhanced (mean sensitivity; 26.3 pg/mL assay, 131 pg/mL; p < 0.01). Both methods were reliable, <10% inter- and intra-assay...

Placental p , ′‐dichlorodiphenyldichloroethylene ( ′‐DDE) concentration and cord blood atopic markers were determined in 19 neonates. Increased placental ′‐DDE was associated with a statistically significant increase plasma interleukin (IL)‐13. Furthermore, both IL‐4/interferon (IFN)‐ γ IL‐13/IFN‐ ratios significantly positively concentration.

Wheat is a prominent allergenic food that can trigger life-threatening anaphylaxis. Presently, it remains unclear whether wheat glutenin (WG) extract possesses inherent sensitization potential independently, without the use of adjuvants, and sensitize mice to extent inducing systemic In this study, we tested hypothesis repeated skin exposures WG adjuvant will with resultant anaphylactic reaction upon challenge. Balb/c were bred maintained on strict plant protein-free diet repeatedly exposed...