A novel biological method for the synthesis of silver nanoparticles using fungus Verticillium is reported. Exposure fungal biomass to aqueous Ag+ ions resulted in intracellular reduction metal and formation dimensions 25 ± 12 nm. Electron microscopy analysis thin sections cells indicated that particles were formed below cell wall surface, possibly due by enzymes present membrane. The not toxic continued multiply after biosynthesis nanoparticles.

Fungi make piles of gold! A green-chemistry route, based on the bioreduction AuCl4− ions by fungus Verticillium sp., for formation gold nanoparticles is demonstrated. The TEM micrograph shows a single cell after reaction with and entrapment wall cytoplasmic membrane.

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTExtracellular Biosynthesis of Monodisperse Gold Nanoparticles by a Novel Extremophilic Actinomycete, Thermomonospora sp.Absar Ahmad, Satyajyoti Senapati, M. Islam Khan, Rajiv Kumar, and Murali SastryView Author Information Biochemical Sciences, Catalysis, Materials Chemistry Divisions, National Chemical Laboratory, Pune − 411 008, India Cite this: Langmuir 2003, 19, 8, 3550–3553Publication Date (Web):March 11, 2003Publication History Received30...

The development of reliable, eco-friendly processes for the synthesis nanoscale materials is an important aspect nanotechnology. In this paper, we report on use alkalotolerant actinomycete (Rhodococcus sp.) in intracellular gold nanoparticles dimension 5–15 nm. Electron microscopy analysis thin sections cells indicated that particles with good monodispersity were formed cell wall as well cytospasmic membrane. are more concentrated cytoplasmic membrane than wall, possibly due to reduction...

A green chemistry approach to nanoparticle synthesis is the exciting possibility opened up by fungus Fusarium oxysporum. The (shown in flask on left), when exposed aqueous AuCl4− ions, reduces metal ions; this leads extracellular formation of gold nanoparticles right).

The biosynthesis of Q-state CdS nanoparticles by reaction aqueous CdSO4 solution with the fungus, Fusarium oxysporum, is demonstrated. Nanoparticle formation proceeds release sulfate reductase enzymes conversion ions to sulfide that subsequently react Cd2+ yield highly stable nanoparticles. Elucidation an enzymatic pathway using fungi opens up exciting possibility developing a rational, strategy for nanomaterials over range chemical compositions which currently not possible.

The exposure of a mixture 1 mM HAuCl4 and AgNO3 solutions to different amounts fungal biomass (Fusarium oxysporum) results in the formation highly stable Au–Ag alloy nanoparticles with dimensions 8–14 nm depending on metal molar fraction (see image). amount cofactor NADH released by F. oxysporum fungus plays an important role controlling composition nanoparticles. Supporting information for this article is available WWW under http://www.wiley-vch.de/contents/jc_2421/2005/z400053_s.pdf or...

A novel approach to the analysis of exosomal RNA is presented using SAW exosome lysis and ion-exchange nanomembrane sensing.

Abstract Extracellular vesicles (EV) containing microRNAs (miRNAs) have tremendous potential as biomarkers for the early detection of disease. Here, we present a simple and rapid PCR-free integrated microfluidics platform capable absolute quantification (<10% uncertainty) both free-floating miRNAs EV-miRNAs in plasma with 1 pM sensitivity. The assay time is only 30 minutes opposed to 13 h requires ~20 μL sample oppose mL conventional RT-qPCR techniques. integrates surface acoustic wave...

Exosomes carry microRNA biomarkers, occur in higher abundance cancerous patients than healthy ones, and because they are present most biofluids, including blood urine, these can be obtained noninvasively. Standard laboratory techniques to isolate exosomes expensive, time consuming, provide poor purity, recover on the order of 25% available exosomes. We a new microfluidic technique simultaneously preconcentrate them by electrophoresis using high transverse local electric field generated...

Abstract Solid-state nanopores allow high-throughput single-molecule detection but identifying and even registering all translocating small molecules remain key challenges due to their high translocation speeds. We show here the same electric field that drives into pore can be redirected selectively pin delay transport. A thin high-permittivity dielectric coating on bullet-shaped polymer permits leakage at tip produce a voltage-dependent surface entry side reversibly edge-pin molecules. This...

Cardiovascular disease-related deaths (one-third of global deaths) can be reduced with a simple screening test for better biomarkers than the current lipid and lipoprotein profiles. We propose using highly atheroprotective subset HDL colocalized PON1 (PON1-HDL) superior cardiovascular risk assessment. However, direct quantification proteomic subclasses are complicated by peroxides/antioxidants associated interfering redox reactions in enzymatic calorimetric electrochemical immunoassays....

Other than concentrating the target molecules at sensor location, we demonstrate two distinct new advantages of an open-flow impedance-sensing platform for DNA hybridization on carbon nanotube (CNT) surface in presence a high-frequency AC electric field. The shear-enhanced and ion transport rate to CNT decouples parasitic double-layer impedance signal from charge-transfer due hybridization. flow field high frequency also amplifies across hybridized provides discrimination between targeted...

The physisorption of negatively charged single-stranded DNA (ssDNA) different lengths onto the surface anion-exchange membranes is sensitively shown to alter anion flux through membrane. At low concentrations, physisorbed DNAs act suppress an electroconvection vortex instability that drives into membrane and hence reduce overlimiting current Beyond a critical concentration, determined by total number phosphate charges on DNA, layer becomes cation-selective membrane, combined bipolar has...

A microfluidic ion exchange membrane hybrid ionic circuit chip is designed to achieve high-flux diode, transistor and amplifier functionalities.

Current biomarkers for myocardial infarction (MI) diagnosis are typically late markers released upon cell death, incapable of distinguishing between ischemic and reperfusion injury can be symptoms other pathologies. Circulating microRNAs (miRNAs) have recently been proposed as alternative MI diagnosis; however, detecting the changes in human cardiac miRNA profile during is extremely difficult. Here, to study levels acute MI, a heart-on-chip model with channel, containing induced pluripotent...

Molecular dielectrophoresis (DEP) is employed to rapidly (<ms) trap ssDNA molecules in a flowing solution cusp-shaped nanocolloid assembly on chip with locally amplified AC electric field gradient. By tuning frequency and DNA DEP mobility relative its electrophoretic due electrostatic repulsion from like-charged nanocolloids, mismatch-specific binding of at the cusp achieved by converging flow, concentration factor about 6 orders magnitude higher than bulk, thus allowing fluorescent...