A5073693478- Electron Spin Resonance Studies
- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Nanoparticles: synthesis and applications
- Electrochemical Analysis and Applications
- Air Quality and Health Impacts
- Advanced Fluorescence Microscopy Techniques
- Force Microscopy Techniques and Applications
- Inhalation and Respiratory Drug Delivery
- TiO2 Photocatalysis and Solar Cells
- Analytical Chemistry and Sensors
- Conducting polymers and applications
- Biosensors and Analytical Detection
- Photosynthetic Processes and Mechanisms
- Retinal Diseases and Treatments
- Protein Interaction Studies and Fluorescence Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Photocatalysis Techniques
- DNA and Nucleic Acid Chemistry
- Antimicrobial agents and applications
- 3D Printing in Biomedical Research
- Advanced NMR Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
Jožef Stefan Institute
2015-2024
Jožef Stefan International Postgraduate School
2005-2024
Infinitesimal (United States)
2024
Centre of Excellence NAMASTE
2011-2018
Austrian Academy of Sciences
2004-2005
Belarusian State University
2005
University of Ljubljana
2005
Nanomaterial (NM) characteristics may affect the pulmonary toxicity and inflammatory response, including specific surface area, size, shape, crystal phase or other characteristics. Grouping of TiO2 in hazard assessment might be challenging because variation physicochemical properties. We exposed C57BL/6 J mice to a single dose four anatase NMs with various sizes shapes by intratracheal instillation assessed 1, 3, 28, 90 180 days post-exposure. The quartz DQ12 was included as benchmark...
Abstract Using site‐directed spin‐labeling EPR spectroscopy, we mapped the region of intrinsically disordered C‐terminal domain measles virus nucleoprotein (N TAIL ) that undergoes induced folding. In addition to four spin‐labeled N variants (S407C, S488C, L496C, and V517C) (Morin et al. (2006), J Phys Chem 110: 20596‐20608), 10 new single‐site cysteine were designed, purified from E. coli, spin‐labeled. These 14 enabled us map in detail gain rigidity presence either secondary structure...
Photocatalytic degradation of dichloroacetic acid (DCA) was studied in a continuous-flow set-up using titanium microreactor with an immobilized double-layered TiO2 nanoparticle/nanotube film. Chloride ions, formed during the process, negatively affect photocatalytic efficiency and at certain concentration (approximately 0.5 mM) completely stop reaction microreactor. Two proposed mechanisms inhibition chloride competitive adsorption photogenerated-hole scavenging, have been investigated by...
Following the widely spread EPR spin-label applications for biosystem characterization, a novel approach is proposed EPR-based characterization of complexity. Hereto computational method based on hybrid evolutionary optimization (HEO) introduced. The enormous volume information obtained from multiple HEO runs reduced with so-called GHOST condensation automatic detection degree system complexity through construction two-dimensional solution distributions. shows ability quantitative groups...
Although the link between inhalation of nanoparticles and cardiovascular disease is well established, causal pathway nanoparticle exposure increased activity blood coagulation factors remains unexplained. To initiate tissue factor bearing epithelial cell membranes should be exposed to blood, on other side less than a micrometre thin air-blood barrier. For inhaled promote coagulation, they need bind lung epithelial-cell membrane parts relocate them into blood. assess this hypothesis, we use...
It is important to understand the nanomaterials intracellular trafficking and distribution investigate their targeting into nuclear area in living cells. In our previous study, we firstly observed penetration of nonmodified positively charged carbon dots decorated with quaternary ammonium groups (QCDs) nucleus mouse NIH/3T3 fibroblasts. Thus, this work, focused on deeper study QCDs inside two healthy L929 cell lines by fluorescence microspectroscopy performed a comprehensive cytotoxic DNA...
TiO2 nanoparticles have generally low toxicity in the vitro systems although some is expected to originate TiO2-associated photo-generated radical production, which can however be modulated by trapping ability of serum proteins. To explore role proteins phototoxicity we measure viability exposed cells depending on nanoparticle and protein concentrations.Fluorescence spin EPR spectroscopy reveal that ratio between concentrations determines amount nanoparticles' surface not covered...
Abstract On a daily basis, people are exposed to multitude of health‐hazardous airborne particulate matter with notable deposition in the fragile alveolar region lungs. Hence, there is great need for identification and prediction material‐associated diseases, currently hindered due lack in‐depth understanding causal relationships, particular between acute exposures chronic symptoms. By applying advanced microscopies omics vitro vivo systems, together silico molecular modeling, it determined...
Fluorescence microspectroscopy (FMS) with environmentally sensitive dyes provides information about local molecular surroundings at microscopic spatial resolution. Until recently, only probes exhibiting large spectral shifts due to changes have been used. For filter-based experimental systems, where signal different wavelengths is acquired sequentially, photostability has required in addition. Herein, we systematically analyzed our fitting models and bleaching correction algorithms which...
Lack of better understanding nanoparticles targeted delivery into cancer cells calls for advanced optical microscopy methodologies.Here we present a development fluorescence microspectroscopy (spectral imaging) based on white light spinning disk confocal microscope with emission wavelength selection by liquid crystal tunable filter.Spectral contrasting images was used to localize polymer and cell membranes labeled fluorophores that have substantially overlapping spectra.In addition, enabled...
We present spectroscopic measurements on the first example of a chemically electron-doped metal-DNA complex. show that doping level as high stoichiometric limit one electron per base pair can be achieved. The doped unpaired electrons occupy lowest unoccupied molecular orbital levels nucleobases detected with spin resonance and x-ray absorption near edge structure measurements. Delocalization strong correlations between are evident from temperature-independent susceptibility microwave...