A5040069227- Reproductive Biology and Fertility
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- S100 Proteins and Annexins
- Ovarian function and disorders
- Assisted Reproductive Technology and Twin Pregnancy
- Drug Transport and Resistance Mechanisms
- Reproductive System and Pregnancy
- Advanced Proteomics Techniques and Applications
- Peptidase Inhibition and Analysis
- Reproductive Health and Technologies
- Muscle Physiology and Disorders
- Sexual function and dysfunction studies
- Pregnancy and preeclampsia studies
- Hemophilia Treatment and Research
- Enzyme Structure and Function
- Homicide, Infanticide, and Child Abuse
- Cell death mechanisms and regulation
- Signaling Pathways in Disease
- Reproductive tract infections research
- Pelvic floor disorders treatments
- Machine Learning in Bioinformatics
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
Dalian Medical University
2013-2021
Second Affiliated Hospital of Chongqing Medical University
2013-2021
Chongqing Medical University
2010-2021
Second Xiangya Hospital of Central South University
2010
Central South University
2010
Legumain is a member of the asparaginyl endopeptidase family that over-expressed in response to hypoxic stress on mammary adenocarcinoma, colorectal cancer, proliferating endothelial cells, and tumor-associated macrophages (TAMs). Here, we demonstrate elevated expression legumain ovarian cancer by proteomic approach using isobaric tags for relative absolute quantification (iTRAQ) followed liquid chromatography-mass spectrometry (LC-MS/MS). To investigate relationship between development,...
In order to understand the molecular mechanisms of multidrug resistance (MDR) in ovarian cancer, we employed proteomic approach isobaric tags for relative and absolute quantification (iTRAQ), followed by LC-MS/MS, using cisplatin-resistant COC1/DDP cell line its parental COC1 as a model. A total number 28 proteins differentially expressed were identified, then differential expression levels partially identified confirmed Western blot analysis and/or real-time RT-PCR. Furthermore, association...
Multi-drug resistance (MDR) is a major obstacle towards successful treatment of hepatocellular carcinoma (HCC). The mechanisms MDR are intricate and have not been fully understood. Therefore, we employed cell-line model consisting the 5-fluorouracil (5-FU) resistant BEL7402/5-FU cell line its parental BEL7402 line. Using relative absolute quantification (iTRAQ)-coupled 2D LC-MS/MS, successfully exploited high-throughput proteomic technology, in total, 660 unique proteins were identified 52...
Quantitative proteomics can be used as a screening tool for identification of differentially expressed proteins potential biomarkers cancers. Here, we comparatively analyzed the proteome profiles ovarian cancer tissues and normal epithelial tissues. Using high-throughput proteomic technology isobaric tags relative absolute quantitation (iTRAQ)-coupled with two-dimensional-liquid chromatography-tandem mass spectrometry, 1,259 unique were identified. Of those, 205 potentially between Several...
Hepatitis B virus (HBV) is the most common of hepatitis viruses that cause chronic liver infections in humans and it considered a major global health problem. However, mechanisms HBV replication are complex not yet fully understood. In this study, DNA-transfected HepG2.2.15 cell line its parental HepG2 were analyzed by isobaric tags for relative absolute quantitation (iTRAQ)-coupled two-dimensional liquid chromatography tandem mass-spectrophotometry (2D LC-MS/MS), successfully exploited...
Multidrug resistance (MDR) is a major obstacle towards successful treatment of gastric cancer. However, the mechanisms MDR are intricate and have not been fully understood. To elucidate molecular in cancer, we employed proteomic approach isobaric tags for relative absolute quantification (iTRAQ), followed by LC-MS/MS, using vincristine-resistant SGC7901/VCR cell line its parental SGC7901 as model. In total, 820 unique proteins were identified 91 showed to be differentially expressed compared...
Hepatitis B virus (HBV) is the most common of hepatitis viruses that cause chronic liver infections in humans, and it considered to be a major global health problem. To gain better understanding HBV pathogenesis, identify novel putative targets for anti-HBV therapy, this study was designed elucidate differential expression host proteins tissue from HBV-transgenic mice. Liver samples two groups, (1) (Tg) mice, (2) corresponding background normal wild-type (WT) were collected subjected iTRAQ...
Ovarian reserve reflects the quality and quantity of available oocytes has become an indispensable measure for better understanding reproductive potential. Proteomic approaches are especially helpful in discerning differential protein expression patterns associated with normal diseased states and, thus, proteomic analyses increasingly used to identify clinically useful biomarkers. The aim this study was investigate proteins secreted urine patients different ovarian by techniques potential...
// Shiying Li 1 , Yixuan Yang Xiangchun Ding 2 Min Sha She Hong Peng Xiaoming Xu Xiaoping Ran Sanglin Hu Huaidong Dazhi Zhang Ren Key Laboratory of Molecular Biology for Infectious Diseases (Ministry Education), Institute Viral Hepatitis, Department Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, PR China General Hospital Ningxia Yinchuan, Ningxia, Correspondence to: Zhang, email: zhangdazhi_1014@163.com Keywords: LHBs, MDR1, HIF-1α, iTRAQ, proteomics...
Despite the use of adjuvant therapies, cumulative proportion live births remains at ~40%. Accumulating data show that low pregnancy rates, even in presence high fertility are due to implantation failure. The present study aimed identify and construct a profile proteins react with preimplantation factor (PIF) provide an understanding into molecular mechanisms by which PIF promotes trophoblast invasion. Cytoplasmic were immunoprecipitated biotin‑labeled synthetic or intralipid scrambled...
The aim of this study was to evaluate the effect chronological age on in vitro fertilization (IVF) outcome patients with diminished ovarian reserve (DOR). Four hundred and forty-nine women DOR who underwent IVF cycles were enrolled study. There only 296 obtained available embryos. no embryos had a significantly lower antral follicle count (AFC) higher basal follicle-stimulating hormone (FSH) concentrations than those embryos, but two groups comparable. However, aged >40 ongoing pregnancy...
Ski is an avian sarcoma virus oncogene homolog best known for inhibiting TGF beta signaling through its association with the SMAD proteins. Anti-Ski antibodies (MAbs) of high titer were prepared by immunizing BALB/c mice multifocal intradermal injections and fusing antibody producing spleen cells myeloma SP2/0 origin. Three MAbs selected further characterization as classes subclasses. Antibodies produced these three clones affinities ranging from 109 to 1011/m. These found be immunoglobulin...
Objective To evaluate the association of vaginal dysbiosis and infertility. Methods Cohort studies, case-control studies cross-sectional were searched in electronic databases, including MEDLINE, EMBASE SinoMed (from inception to October 2017); addition, reference lists included similar reviews handsearched. These investigating correlation between infertility included. The Meta-Analysis was performed by RevMan 5.3 software. Results 1 724 records retrieved search; from those, 9...