A5066291252- Influenza Virus Research Studies
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Erythrocyte Function and Pathophysiology
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Immune Response and Inflammation
- Hepatitis C virus research
- Mosquito-borne diseases and control
- Agricultural Engineering and Mechanization
- Mast cells and histamine
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Soil Mechanics and Vehicle Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
Shizuoka Medical Center
2024
Airlangga University
2016-2023
Kobe University
2014-2023
International Medical Research (Germany)
2021
Nihon University
2004-2018
Bio-oriented Technology Research Advancement Institution
2006-2014
Institute of Agricultural Machinery
2010-2014
National Agriculture and Food Research Organization
2010-2014
Bipar
2008
Tokai University
1976-2003
Human B-cell differentiation factor (BCDF) was purified to homogeneity by sequential filtration and chromatography of culture supernatants from TCL-Na1 cells on an AcA34 gel column then a Mono P with fast protein liquid reversed-phase HPLC. A 5300-fold enrichment in specific activity BCDF about 25% recovery attained. The evidenced the following: (i) 1.7 X 10(7) units/mg protein, (ii) only two bands, Mr 19,000 21,000, were identified NaDodSO4/PAGE under reduced as well nonreduced conditions,...
Aim: With advent of reverse‐transcription (RT)/polymerase chain reaction (PCR) for detection the hepatitis E viral genome, we carried out retrospective examinations. Methods: Serum samples collected from 68 patients diagnosed as with unknown etiology were tested markers virus. Results: Two them found positive RNA. While clinical course one patient (patient 1) was typical acute E, another 2) persistently infected HEV. Patient 2 virus via blood transfusion during chemotherapy against T‐cell...
Abstract To halt the pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), governments around world have imposed policies, such as lockdowns, mandatory mask wearing, and social distancing. The application disinfecting materials in shared public facilities can be an additional measure to control spread virus. Copper is a prominent material with antibacterial antiviral effects. In this study, we synthesized copper nanoparticles (CuNPs) surface coating agent assessed their...
Abstract Airway epithelial cells which are the initial site of influenza virus (IV) infection suggested to participate in airway inflammatory response by expressing various cytokines including RANTES; however, intracellular signal that regulates RANTES expression has not been determined. In present study, we examined role p38 mitogen-activated protein (MAP) kinase, extracellular signal-regulated kinase (Erk), and c-Jun-NH2-terminal (JNK) production IV-infected human bronchial cells. The...
Abstract Background Coronavirus disease 2019 (COVID-19) is a global health problem that causes millions of deaths worldwide. The clinical manifestation COVID-19 widely varies from asymptomatic infection to severe pneumonia and systemic inflammatory disease. It thought host genetic variability may affect the host’s response virus thus cause severity SARS-CoV-2 requires interaction with its receptor complex in cells before infection. transmembrane protease serine 2 (TMPRSS2) has been...
In a previous study, we demonstrated that humanized NOD/SCID/IL2Rgamma(null) (hNOG) mice constructed with human hematopoietic stem cells (HSCs) allow efficient immunodeficiency virus type 1 (HIV-1) infection. However, HIV-1 infection could be monitored for only 43 days in the animals due to their short life spans. By transplanting HSCs without any myeloablation methods, successfully survived longer than 300 stable engraftment of cells. The showed high viremia state more 3 months examined,...
Gold nanoparticles were conjugated to an antibody (immuno-AuNP) against A/Udorn/307/1972 (H3N2) influenza virus detect viruses on a sensing plate designed for evanescent field-coupled waveguide-mode sensor. Experiments conducted using human A/H3N2 strains, and immuno-AuNP could 8×10(5) PFU/ml (40 pg/µl) intact 120 pg/µl A/Brisbane/10/2007. Furthermore, increased signal magnitude was achieved in the presence of non-ionic detergent, as virtual detection level 8×10(4) A/Udorn/307/1972....
Influenza virus neuraminidase (NA) cleaves terminal sialic acid residues on oligosaccharide chains that are receptors for binding, thus playing an important role in the release of virions from infected cells to promote spread cell-to-cell infection. In addition, NA plays a at initial stage viral infection respiratory tract by degrading hemagglutination inhibitors body fluid which competitively inhibit receptor binding virus. Current first line anti-influenza drugs NA-specific inhibitors, do...
Understanding the co-occurrence of respiratory viruses is crucial for patient management and effective control strategies. This study aimed to retrospectively reveal prevalence influenza A B among suspected COVID-19 patients. We examined presence Influenza RNAs in 1099 remnant samples diagnostic patients during first wave East Java using a multiplex PCR one-step TaqMan real-time systems. found that 7.3% 1.5% subjects were positive B, respectively, while 13.1% had previously tested...
The envelopes of HVJ (Sendai virus) virions were solubilized with alkali-Emasol treatment. envelope subunit(s) associated hemagglutination-inhibiting antibody blocking, neuraminidase, and low hemagglutinating (HA) activities had a sedimentation coefficient 8.8 S . Envelope fragment-like structures assembled from the subunits after Emasol was removed by gel filtration. These reassembled particles HA activity cell-fusion as well hemolytic activity. Sodium dodecyl sulfate-polyacrylamide...
Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among diseases, influenza viral infections are worldwide cause pandemic disease mortality. With emergence different strains, it vital develop method using antibodies that can differentiate between types subtypes. Ideally, such system would also be user friendly. In this study, polyclonal antibody generated...
Summary Background Previous studies have shown that rat peritoneal mast cells and cell model basophilic leukaemia (RBL‐2H3) generate intracellular reactive oxygen species (ROS) in response to antigen challenge. However, the physiological significance of burst ROS is poorly understood. Objective The present study was undertaken investigate role superoxide anion mediator release human systems. Methods RBL‐2H3 were directly stimulated with anti‐rat FcεRI α‐subunit monoclonal antibody (mAb). For...
The surfaces of silica-based sensor chips, designed for evanescent-field-coupled waveguide-mode sensors, were functionalized using various surface chemistries. immobilization molecular entities on the silica was monitored microscopic techniques (scanning electron, fluorescence, and atomic force microscopies). Further, gold nanoparticle-based signal enhancement analyses performed with protein conjugation different a sensor. Based these analyses, modified glutaraldehyde (Glu)...
Glycosphingolipids form glycosphingolipid signaling microdomains. Here, we report an unrecognized type of phosphatidylglucoside (PhGlc)-based lipid microdomain in HL60 cells. Treatment cells with rGL-7, which preferentially reacts PhGlc, induced differentiation This was manifested by the appearance nitroblue tetrazolium-positive together CD38 expression and c-Myc down-regulation. We determined molecular mechanisms underlying early stages signal transduction. rGL-7 treatment rapid tyrosine...
Summary Background Influenza virus (IV) infection causes airway inflammation; however, it has not been determined whether IV could catabolize arachidonic acid cascade in epithelial cells. In addition, the responsible intracellular signalling molecules that have determined. Objective present study, to clarify these issues, we examined cyclooxygenase (COX) expression, cytosolic phospholipase A 2 (cPLA ) phosphorylation and prostaglandin E (PGE release human bronchial cells (BEC) upon...
IgG immune complexes trigger humoral responses by cross-linking of FcRs for (FcgammaRs). In the present study, we investigated role lipid rafts, glycolipid- and cholesterol-rich membrane microdomains, in FcgammaR-mediated responses. retinoic acid-differentiated HL-60 cells, FcgammaRs resulted a marked increase tyrosine phosphorylation FcgammaRIIa, p58(lyn), p120(c-cbl), which was inhibited specific inhibitor Src family protein kinases. After cross-linking, tyrosine-phosphorylated proteins...