A5036954013- Calcium signaling and nucleotide metabolism
- Cellular transport and secretion
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- RNA modifications and cancer
- Sphingolipid Metabolism and Signaling
- Nitric Oxide and Endothelin Effects
- Heat shock proteins research
- Neonatal Health and Biochemistry
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Lysosomal Storage Disorders Research
- PARP inhibition in cancer therapy
- Enzyme Structure and Function
- Neuroscience of respiration and sleep
- Cancer therapeutics and mechanisms
- Traditional Chinese Medicine Analysis
- Oral microbiology and periodontitis research
- Advanced Glycation End Products research
Tokyo Medical and Dental University
2013-2024
The University of Tokyo
1984-2021
Kansai Medical University
2015-2020
Shinshu University
2014
Kameda Medical Center
2014
Osaka National Hospital
2014
Nagoya University
2014
Tokushima University
2010
Ochanomizu University
2004
Nihon University
1989-2003
1. We examined the role of nitric oxide (NO) in respiratory regulation nucleus tractus solitarii (NTS), where L‐glutamate release associated with peripheral chemoreceptor activation modulates hypoxic ventilatory response. 2. Experiments were performed unanaesthetized freely moving rats. First, effects on response sodium nitroprusside (SNP, a NO donor) or NG‐monomethyl‐L‐arginine (L‐NMMA, synthase inhibitor), microinjected into NTS, investigated. Second, using vivo microdialysis, changes...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo tRNA1Ile species from an extreme thermophile, Thermus thermophilus HB8: effect of 2-thiolation ribothymidine on the thermostability tRNANobuyuki Horie, Miki Hara-Yokoyama, Shigeyuki Yokoyama, Kimitsuna Watanabe, Yoshiyuki Kuchino, Susumu Nishimura, and Tatsuo MiyazawaCite this: Biochemistry 1985, 24, 21, 5711–5715Publication Date (Print):October 1, 1985Publication History Published online1 May 2002Published inissue 1 October...
Interleukin-5 (IL-5), a major hematopoietin, stimulates eosinophil proliferation, migration, and activation, which have been implicated in the pathogenesis of allergic inflammatory diseases, such as asthma. The specific IL-5 receptor (IL-5R) consists α subunit (IL-5RA) common β (βc). binding to IL-5R on target cells induces rapid tyrosine phosphorylation activation various cellular proteins, including JAK1/JAK2 STAT1/STAT5. Here, we report crystal structure dimeric complex with IL-5RA...
Amylase exocytosis of the parotid gland is mediated by intracellular cAMP. To investigate whether cAMP-dependent secretion has a mechanism similar to that regulated neuroexocytosis, we examined expression synaptosome-associated proteins. In rat acinar cells, found 25 (p25) and 18 kDa (p18) proteins reacted with antibodies against Rab3A vesicle-associated membrane protein 2 (VAMP-2), respectively. On other hand, syntaxin 1 SNAP-25, which interact VAMP-2 at synapses, were undetectable....
Glycosphingolipids form glycosphingolipid signaling microdomains. Here, we report an unrecognized type of phosphatidylglucoside (PhGlc)-based lipid microdomain in HL60 cells. Treatment cells with rGL-7, which preferentially reacts PhGlc, induced differentiation This was manifested by the appearance nitroblue tetrazolium-positive together CD38 expression and c-Myc down-regulation. We determined molecular mechanisms underlying early stages signal transduction. rGL-7 treatment rapid tyrosine...
IgG immune complexes trigger humoral responses by cross-linking of FcRs for (FcgammaRs). In the present study, we investigated role lipid rafts, glycolipid- and cholesterol-rich membrane microdomains, in FcgammaR-mediated responses. retinoic acid-differentiated HL-60 cells, FcgammaRs resulted a marked increase tyrosine phosphorylation FcgammaRIIa, p58(lyn), p120(c-cbl), which was inhibited specific inhibitor Src family protein kinases. After cross-linking, tyrosine-phosphorylated proteins...
High-mobility group box-1 (HMGB1) protein acts as a transcription factor in the nucleus and also pro-inflammatory cytokine when released into extracellular fluids. The presence of higher levels HMGB1 is reported gingival crevicular fluid from periodontal patients. Since proliferation bacteria within pocket closely involved exacerbation disease, it hypothesized that causes release HMGB1. Immunohistochemical staining inflamed gingiva revealed exclusively dislocated to cytoplasm epithelium,...
LAMP1 (lysosomal-associated membrane protein 1) and LAMP2 are the most abundant components of lysosome membranes. Both LAMPs have common structures consisting a large lumenal domain composed two domains (N-domain C-domain, which membrane-distal -proximal, respectively), both with β-prism fold, transmembrane domain, short cytoplasmic tail. is involved in various aspects autophagy, reportedly forms high-molecular weight complexes at lysosomal membrane. We previously showed that molecules...
Amylase release from parotid acinar cells is mainly induced by the accumulation of intracellular cAMP, presumably through phosphorylation substrates cAMP-dependent protein kinase (PKA). However, molecular mechanisms this process are not clear. In a previous study (Fujita-Yoshigaki, J., Dohke, Y., Hara-Yokoyama, M., Kamata, Kozaki, S., Furuyama, and Sugiya, H. (1996) J. Biol. Chem. 271, 13130–13134), we reported that vesicle-associated membrane 2 (VAMP2) localized at secretory granule...
We have recently reported that gangliosides act as inhibitors of ADP-ribosyltransferases and NAD+ glycohydrolases (NADase) pertussis toxin the C3 exoenzyme from Clostridium botulinum (Hara-Yokoyama, M., Hirabayashi, Y., Irie, F., Syuto, B., Moriishi, K., Sugiya, H., Furuyama, S. (1995) J. Biol. Chem. 270, 8115-8121). Here, we investigated effect on enzymatic activity leukocyte cell surface antigen CD38, which is identified an ecto-NADase (Kontani, Nishina, Ohoka, Takahashi, Katada, T. (1993)...
The arrangement of immature germ cells changes regularly and periodically along the axis seminiferous tubule, is used to describe progression spermatogenesis. This description based primarily on in acrosome nuclear morphology haploid spermatids. However, such criteria cannot be applied under pathological conditions with arrested spermatid differentiation. In settings, associated differentiation premeiotic must analyzed. Here, we found that unique bipolar motor protein, KIF11 (kinesin-5/Eg5),...
Abstract Claudins are the major component of tight junctions, which form a primary barrier to paracellular diffusion and maintain cell polarity in normal epithelia endothelia. In cancer cells, claudins play additional roles besides serving as components participate anoikis or invasion. Among claudin family proteins, claudin‐1 has most promising potential, both diagnostically prognostically, many types cancers, including oral, gastric, liver, colon cancers. However, conflicting results have...
Abstract Genetic code expansion enables site‐specific photo‐crosslinking by introducing photo‐reactive non‐canonical amino acids into proteins at defined positions during translation. This technology is widely used for analyzing protein–protein interactions and applicable in mammalian cells. However, the identification of crosslinked region still remains challenging. Here, we developed a new method to identify pre‐installing cleavage site, an α‐hydroxy acid ( N ε...
Heparan sulfate proteoglycans (HSPGs) are one of the basic constituents plasma membranes. Specific molecular interactions between HSPGs and a number extracellular ligands have been reported. Mechanisms involved in controlling localization abundance HSPG on specific domains cell surface, such as membrane rafts, could play important regulatory roles signal transduction.Using metabolic radiolabeling sucrose-density gradient ultracentrifugation techniques, we identified [(35)S]sulfate-labeled...
Heat shock cognate protein 70 (Hsc70/HSPA8) belongs to the Hsp70 family of molecular chaperones. The fundamental functions chaperones depend on ATP-dependent allosteric regulation binding and release hydrophobic polypeptide substrates. Hsc70 is also involved in various other cellular including selective pathways degradation: chaperone-mediated autophagy (CMA) endosomal microautophagy (eMI), which recruits substrate proteins containing a KFERQ-like pentapeptide motif from cytosol lysosomes...
The gene for the Glu‐tRNA synthetase from an extreme thermophile, Thermus thermophilus HB8, was isolated using a synthetic oligonucleotide probe coding N‐terminal amino acid sequence of synthetase. Nucleotide‐sequence analysis revealed open reading frame protein composed 468 residues ( M r 53901). Codon usage in T. fact similar to characteristic usages genes proteins bacteria genus : G + C content third position codons as high 94%. In contrast, showed similarity with bacterial synthetases...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConformation change of tRNAGlu in the complex with glutamyl-tRNA synthetase is required for specific binding L-glutamateMiki Hara-Yokoyama, Shigeyuki Yokoyama, and Tatsuo MiyazawaCite this: Biochemistry 1986, 25, 22, 7031–7036Publication Date (Print):November 4, 1986Publication History Published online1 May 2002Published inissue 4 November 1986https://pubs.acs.org/doi/10.1021/bi00370a041https://doi.org/10.1021/bi00370a041research-articleACS...