A5039966471- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Liver Disease and Transplantation
- Organ Donation and Transplantation
- Xenotransplantation and immune response
- Anatomy and Medical Technology
- Surgical Simulation and Training
- Innovations in Medical Education
- Organ and Tissue Transplantation Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Gallbladder and Bile Duct Disorders
- Melanoma and MAPK Pathways
- Synthesis and biological activity
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Gastrointestinal disorders and treatments
- Immune Cell Function and Interaction
- Liver Disease Diagnosis and Treatment
- Microbial Natural Products and Biosynthesis
- Protein Kinase Regulation and GTPase Signaling
- Cytokine Signaling Pathways and Interactions
- Viral-associated cancers and disorders
- CRISPR and Genetic Engineering
- Acute Kidney Injury Research
- Biomedical Text Mining and Ontologies
Emory University
2013-2024
University of Wisconsin–Madison
2020-2022
Johns Hopkins University
2021
Grady Memorial Hospital
2021
Emory University Hospital
2013-2015
University of British Columbia
2005-2006
University of Amsterdam
2005
Pearl River Community College
2001
University of Utah
2001
University of the Philippines Diliman
2001
The shortage of available organs remains the greatest barrier to expanding access transplant. Despite advances in genetic editing and immunosuppression, survival experimental models kidney xenotransplant has generally been limited <100 days. We found that pretransplant selection recipients with low titers anti-pig antibodies significantly improved a pig-to–rhesus macaque transplant model (6 days vs median time 235 days). Immunosuppression included transient pan–T cell depletion an...
Xenotransplantation using pig organs could end the donor organ shortage for transplantation, but humans have xenoreactive antibodies that cause early graft rejection. Genome editing can eliminate xenoantigens in pigs to minimize impact of these xenoantibodies. Here we determine whether an improved cross-match and chemical immunosuppression result prolonged kidney xenograft survival a pig-to-rhesus preclinical model.
Objective: To define benchmark values for liver transplantation (LT) in patients with perihilar cholangiocarcinoma (PHC) enabling unbiased comparisons. Background: Transplantation PHC is used reluctance many centers and even contraindicated several countries. Although LT are available, there a lack of specific data on performed PHC. Methods: considered after Mayo-like protocol were analyzed 17 reference 2 continents over the recent 5-year period (2014–2018). The minimum follow-up was 1 year....
Liphagal (1), a selective inhibitor of PI3K α, has been isolated from the marine sponge Aka coralliphaga collected in Dominica. The "liphagane" meroterpenoid carbon skeleton liphagal (1) is new. A biomimetic total synthesis used to confirm constitution and support proposed biogenesis.
Raf/MEK-1/MAPK cascade inhibitor activity-directed fractionation of the sponge Stylissa massa afforded eight known alkaloids: aldisine (1), 2-bromoaldisine (2), 10Z-debromohymenialdisine (3), 10E-hymenialdisine (4), 10Z-hymenialdisine (5), hymenin (6), oroidin (7), and 4,5-dibromopyrrole-2-carbonamide (8). Both 4 5 showed significant enzyme inhibitory activity (IC50 3 6 nM, respectively). Secondary assays identified these compounds as potent MEK-1 inhibitors. Compounds also inhibited growth...
Interrupting T cell costimulatory signals as a strategy to control undesired immune responses, such occur in autoimmunity or transplantation, has the potential alleviate many of unwanted side effects associated with current immunosuppressive therapies. Belatacept, high-affinity version CTLA4-Ig that blocks ligand ligation CD28, been approved for use kidney transplant recipients. Despite long-term benefits its use, improved renal function and lower cardiovascular risk, subset patients treated...
ABSTRACT Objective To evaluate the clinically relevant anti‐CD40 antibody iscalimab for baseline immunosuppression in a preclinical pig‐to‐rhesus renal xenograft model. Summary Background Data CD40/CD40L co‐stimulation blockade‐based has been more successful than calcineurin‐based protocols prolonging survival models. Methods GGTA1 knockout/CD55 transgenic pig kidneys were transplanted into rhesus monkeys ( n = 6) receiving an iscalimab‐based immunosuppressive regimen. Results Two grafts...
Despite the increased usage of livers from donation after circulatory death (DCD) donors in last decade, many patients remaining on waitlist who need a liver transplant. Recent efforts have focused maximizing utilization and outcomes these allografts using advances machine perfusion technology other perioperative strategies such as normothermic regional (NRP). In addition to standard donor recipient matching that is required with DCD donation, new data regarding impact graft steatosis,...
Large single-institution series of patients undergoing resection for gastrointestinal stromal tumors (GIST) are lacking. Clinicopathologic characteristics and postoperative outcomes were retrospectively collected analyzed from GIST 2002 to 2011. One hundred seventy-six identified; 156 underwent primary nonmetastatic disease. KIT mutations identified in 131 (84.0%). Of the with disease, most common site was stomach (75.6%). Tumors categorized as very low (24.4%), (35.9%), intermediate...
Phosphoinositide 3-kinases (PI3Ks) comprise a family of kinases that transfer the terminal phosphate adenosine triphosphate to phosphoinositides at 3-hydroxyl inositol ring form phosphoinositide (3,4,5) (PIP3). The PI3Ks have been shown play key roles in cell growth, motility, morphology, and survival thus are interest as targets anti-inflammatory anti-oncogenic drug development. To facilitate identification novel selective inhibitors PI3Ks, we developed TR-FRET assay uses directly labeled...
To investigate the optimal timing of direct acting antiviral (DAA) administration in patients with hepatitis C-associated hepatocellular carcinoma (HCC) undergoing liver transplantation (LT).In C (HCV) associated HCC LT, direct-acting antivirals to achieve sustained virologic response (SVR) and improved oncologic outcomes remains a topic much debate.The United States LT Consortium (2015-2019) was reviewed for primary HCV-associated who underwent received DAA therapy at 20 institutions....
Costimulation blockade using belatacept results in improved renal function after kidney transplant as well decreased likelihood of death/graft loss and reduced cardiovascular risk; however, higher rates grades acute rejection have prevented its widespread clinical adoption. Treatment with blocks both positive (CD28) negative (CTLA-4) T cell signaling. CD28-selective therapies may offer potency by blocking CD28-mediated costimulation while leaving CTLA-4 mediated coinhibitory signals intact....
Background The interplay between viral infection and alloimmunity is known to influence the fate of transplanted organs. Clarifying how local virus-associated inflammation/injury antiviral immunity can alter host alloimmune responses in transplantation remains a critical question. Methods We used mouse model polyomavirus (PyV) kidney investigate roles direct pathology, immune response, pathogenesis PyV-associated allograft injury. have previously shown that an effective primary T cell...
Mixed lymphohematopoietic chimerism is a proven strategy for achieving operational transplant tolerance, though the underlying immunologic mechanisms are incompletely understood. A post-transplant, non-myeloablative, tomotherapy-based total lymphoid (TLI) irradiation protocol combined with anti-thymocyte globulin and T cell co-stimulatory blockade (belatacept) induction was applied to 3-5 MHC antigen mismatched rhesus macaque kidney hematopoietic model. Mechanistic investigations of early...
Takotsubo cardiomyopathy (TCM) is an acute, stress-mediated, reversible that occurs in the absence of hemodynamically significant coronary artery disease. We aimed to investigate characteristics and outcomes patients who developed TCM following liver transplantation (LT) a multicenter study. Adult from 6 centers across United States according Mayo Clinic criteria LT between 2008 2023 were included. Demographics, perioperative long-term outcomes, treatment modalities assessed. Fifty-five The...
Objective: To investigate the long-term outcomes of patients with combined primary sclerosing cholangitis/inflammatory bowel disease (PSC-IBD) undergoing both liver transplantation (LT) and total abdominal colectomy (TAC). Summary Background Data: The fraction PSC-IBD that require LT TAC is small, thereby limiting significant conclusions regarding outcomes. Methods: Adult pediatric from nine centers US IBD Surgery Collaborative who underwent staged for were included. Long-term outcomes,...