A5082133160- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Antioxidant Activity and Oxidative Stress
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Garlic and Onion Studies
- NF-κB Signaling Pathways
- Neuroendocrine Tumor Research Advances
- Synthesis and biological activity
- Cytokine Signaling Pathways and Interactions
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- interferon and immune responses
- Nonmelanoma Skin Cancer Studies
- PI3K/AKT/mTOR signaling in cancer
- Gene Regulatory Network Analysis
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Ginger and Zingiberaceae research
- Cancer Research and Treatments
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Adenosine and Purinergic Signaling
- Data Visualization and Analytics
- Nuclear Receptors and Signaling
National Cancer Institute
2022-2024
United States Army Institute of Surgical Research
2022-2024
National Institutes of Health
2024
Adventist HealthCare Shady Grove Medical Center
2024
Center for Environmental Health
2017-2020
United States Army
1994-2020
Louisiana State University Health Sciences Center Shreveport
2005-2012
Louisiana State University in Shreveport
2011
The University of Texas MD Anderson Cancer Center
1990-2007
Louisiana State University Health Sciences Center New Orleans
2006-2007
Insulin-like growth factor-binding protein (IGFBP)-3 regulates apoptosis in an IGF-independent fashion and has been shown to localize nuclei. We cloned the nuclear receptor retinoid X receptor-alpha(RXR-alpha) as IGFBP-3 partner a yeast two-hybrid screen. Multiple methodologies showed that RXR-alpha bind each other within nucleus. IGFBP-3-induced was abolished RXR-alpha-knockout cells. RXR ligands were additive inducing prostate cancer enhanced response element inhibited RARE signaling....
Lung cancers develop via multiple genetic and epigenetic changes, including inactivation of tumor suppressor genes. We previously cloned human G protein–coupled receptor family C type 5A (GPRC5A), whose expression is suppressed in some lung carcinoma cells, its mouse homolog Gprc5a. generated Gprc5a knockout mice by homologous recombination studied their phenotype macroscopic observation microscopic histologic analysis embryos lungs 1- to 2-year-old mice. GPRC5A mRNA was analyzed reverse...
The F9 murine embryonal carcinoma cell line represents a well-established system for the study of retinoid signaling in vivo. We have investigated functional specificity different X receptor (RXR)-retinoic acid (RA) (RAR) isotype pairs control expression endogenous RA-responsive genes, by using wild-type (WT), RXRα−/−, RARα−/−, RARγ−/−, RXRα−/−-RARα−/−, and RXRα−/−-RARγ−/− cells, as well panRXR RAR (α, β, γ)-selective retinoids. show that these cells sets genes is preferentially mediated...
PURPOSE: The purpose of this study was to test interferon alfa (IFNα), 13-cis-retinoic acid (13cRA), and cisplatin biochemotherapy in advanced squamous cell carcinoma (SCC) the skin. PATIENTS AND METHODS: Patients with skin SCC received IFNα (5 × 106 IU/m2, subcutaneous injection, three times a week), 13cRA (1 mg/kg, orally, daily), (20 mg/m2, intravenous weekly) phase II trial. growth inhibition, cell-cycle, apoptosis activity these agents evaluated two lines (SRB1-m7 SRB12-p9). RESULTS:...
Dextran sodium sulfate (DSS)-induced colitis is widely used to study pathological mechanisms and potential treatments of inflammatory bowel disease. Because temporal changes in genome expression profiles remain unknown this model, we performed whole profile analysis during the development DSS comparison with ulcerative (UC) specimens identify novel common responses Colon tissue from DSS-treated mice was collected at days 0, 2, 4, 6. Half each specimen for histopathological half Affymetrix...
PURPOSE: The purpose of this study was to test interferon alfa (IFNα), 13-cis-retinoic acid (13cRA), and cisplatin biochemotherapy in advanced squamous cell carcinoma (SCC) the skin. PATIENTS AND METHODS: Patients with skin SCC received IFNα (5 × 10 6 IU/m 2 , subcutaneous injection, three times a week), 13cRA (1 mg/kg, orally, daily), (20 mg/m intravenous weekly) phase II trial. growth inhibition, cell-cycle, apoptosis activity these agents evaluated two lines (SRB1-m7 SRB12-p9). RESULTS:...
Cellular stresses inhibit retinoid signaling, but the molecular basis for this phenomenon has not been revealed. Here, we present evidence that X receptor (RXR) is a substrate both mitogen-activated protein kinase kinase-4 (MKK4/SEK1) and its downstream mediator c-Jun N-terminal (JNK). MKK4/SEK1 JNK recognized distinct features on RXR in DE AB regions, respectively. Phosphorylation by had profound effects biochemical properties of RXR, inhibiting expression genes activated RXR-retinoic acid...
We have generated F9 murine embryonal carcinoma cells in which either the retinoid X receptor (RXR)α and retinoic acid (RAR)α genes or RXRα RARγ are knocked out, compared their phenotypes with those of wild-type (WT), RXRα−/−, RARα−/−, RARγ−/− cells. RXRα−/−/ RARα−/− were resistant to treatment for induction primitive parietal endodermal differentiation, as well antiproliferative apoptotic responses, whereas they could differentiate into visceral endodermlike cells, previously observed...
Constitutive activation of signal transducer and activator transcription 3 (Stat3) has been found in a variety human malignancies suggested to play an important role carcinogenesis. Recently, our laboratory demonstrated that Stat3 is required for the development skin tumors via two-stage carcinogenesis using skin-specific loss-of-function transgenic mice. To investigate further each stage chemical mouse skin, i.e. initiation promotion stages, we generated inducible Stat3-deficient mice...
Objective Squamous cell carcinoma (SCCa) has increased from 4% to 10% over 4 decades, stimulating interest in developing novel agents that slow sun‐damaged skin progression. This is the first study evaluating naturally occurring bioactive food compound curcumin on cancer xenografts. Low bioavailability of slowed its transition clinical trials. It hypothesized growth‐inhibitory effects through MTOR pathway and chemopreventive potential SCCa where local application could bypass problems. Study...
Glycogen synthase kinase-3β (GSK-3β) activity is suppressed when it becomes phosphorylated on serine 9 by protein kinase B (Akt). To determine how GSK-3β opposes Akt function we used various methods to alleviate suppression in prostate carcinoma cells. In some experiments, LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (a involved activating Akt) and tumor necrosis factor-α (TNF-α) were activate GSK-3β. other experiments mutant forms GSK-3β, GSK-3βΔ9 constitutively active...
Retinoids inhibit the growth and enhance differentiation of murine S91-C2 melanoma cells. Specific alterations in gene expression are a plausible mechanism for these effects. Since nuclear retinoic acid receptors (RAR) likely mediators retinoid-induced changes expression, we used Northern blotting to analyze RARα, RARβ, RARγ mRNA both RARα was detected cells, but no RARβ could be found. Treatment with 10−7 10−6m β-all-trans-retinoic (RA) 24 h caused 1.5- 2-fold increase mRNA, whereas lower...
Abstract Background Retinoids have been studied extensively for their potential as therapeutic and chemopreventive agents a variety of cancers, including nonmelanoma skin cancer (NMSC). Despite use many years, the mechanism action retinoids in prevention NMSC is still unclear. In this study we attempted to understand all- trans retinoic acid (ATRA), primary biologically active retinoid, order more efficiently utilize clinic. Results We used 2-stage dimethylbenzanthracene (DMBA)/12- O...
Life science research is moving quickly towards large-scale experimental designs that are comprised of multiple tissues, time points, and samples. Omic time-series experiments offer answers to three big questions: what collective patterns do most analytes follow, which follow an identical pattern or synchronize across cohorts, how biological functions evolve over time. Existing tools fall short robustly answering visualizing all questions in a unified interface. Functional Heatmap offers...
The recent advancement of microarray technology with lower noise and better affordability makes it possible to determine expression several thousand genes simultaneously. differentially expressed are filtered first then clustered based on the profiles genes. A large number clustering algorithms distance measuring matrices proposed in literature. popular ones among them include hierarchal k-means clustering. These have often used Euclidian or Pearson correlation distance. biologists...
Eukaryotic initiation factor 4E (eIF4E) is elevated in many cancers and a prognostic indicator breast cancer. Many pro-tumorigenic proteins are selectively translated via eIF4E, including c-Myc, cyclin D1, ornithine decarboxylase (ODC), vascular endothelial growth (VEGF) Tousled-like kinase 1B (TLK1B). However, western blot analysis of these factors human cancer has been limited by the availability fresh frozen tissue labor-intensive nature multiple assays required. Our goal was to validate...
Abstract Squamous cell carcinoma (SCC) of the skin is most clinically aggressive form nonmelanoma cancer. We have determined effects all-trans retinoic acid (ATRA), a naturally occurring chemopreventive retinoid, on signal transducer and activator transcription 3 (Stat3) signaling during development SCC. Stat3 factor that plays critical role in proliferation survival, it constitutively active several malignant types. previously shown required for initiation, promotion, progression ATRA...