Rather than being a passive, haphazard process of wear and tear, lifespan can be modulated actively by components the insulin/insulin-like growth factor I (IGFI) pathway in laboratory animals. Complete or partial loss-of-function mutations genes encoding insulin/IGFI result extension life span yeasts, worms, flies, mice. This remarkable conservation throughout evolution suggests that altered signaling this may also influence human lifespan. On other hand, evolutionary tradeoffs predict...

Insulin-like growth factor-binding protein (IGFBP)-3 regulates apoptosis in an IGF-independent fashion and has been shown to localize nuclei. We cloned the nuclear receptor retinoid X receptor-alpha(RXR-alpha) as IGFBP-3 partner a yeast two-hybrid screen. Multiple methodologies showed that RXR-alpha bind each other within nucleus. IGFBP-3-induced was abolished RXR-alpha-knockout cells. RXR ligands were additive inducing prostate cancer enhanced response element inhibited RARE signaling....

Abstract Background & Aims Mitochondrial dynamics of fission and fusion plays critical roles in a diverse range important cellular functions, its deregulation has been increasingly implicated human diseases. Previous studies have shown that increased mitochondrial significantly promoted the proliferation hepatocellular carcinoma (HCC) cells. However, how they influence migration tumour cells remained largely unknown. Methods In present study, we further investigated effect on metastasis...

BACKGROUND:Accumulating evidence suggests the involvement of long non-coding RNAs (lncRNAs) as oncogenic or tumor suppressive regulators in development various cancers. In present study, we aimed to identify a lncRNA signature based on RNA sequencing (RNA-seq) data predict survival esophageal cancer. MATERIAL AND METHODS:The RNA-seq expression and clinical information were downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed lncRNAs screened out between cancer...

Colorectal cancer (CRC) is one of the most leading causes deaths worldwide. In study, we aimed to identify key long non-coding RNAs (lncRNAs) significantly associated with prognosis CRC and develop an expression-based lncRNA signature provide survival risk prediction for patients. LncRNA expression profiles clinical information patients were collected from The Cancer Genome Atlas (TCGA) database. Six hundred eighteen differentially expressed lncRNAs identified between normal tissues. After...

Mesenchymal stem cell (MSC) transplantation has emerged as an option for the treatment of chronic hepatic cirrhosis, while its therapeutic efficacy could be improved. The bcl-2 gene is anti-apoptotic and can help survival proliferation. Therefore, we explored whether transplanted MSCs with enhanced expression may beneficial in experimental cirrhosis rats.MSCs were isolated from rat bone marrow, expanded vitro transfected adeno-associated virus (AAV) engineered (AAV-bcl-2). Rats induced by...

This study was aimed to develop a lncRNA-associated competing endogenous RNA (ceRNA) network provide further understanding of the ceRNA regulatory mechanism and pathogenesis in colorectal cancer (CRC).Expression profiles mRNAs, lncRNAs, miRNAs, clinical information for CRC patients were obtained from The Cancer Genome Atlas. differentially expressed miRNAs (referred as "DEmRNAs", "DElncRNAs", "DEmiRNAs", respectively) screened out between 539 samples 11 normal samples. interactions DElncRNAs...

The epithelial-mesenchymal transition (EMT) is a pivotal process for fibrotic disease, embryonic development, and wound healing. Moreover, some evidence has proven that the disorder of EMT also plays an important role in carcinogenesis, especially invasion metastasis various tumors (Ritchie et al., 2015). Additionally, gastric adenocarcinoma (GAC) common gastrointestinal malignancy which fourth most commonly diagnosed tumor. Our study aimed at identifying prognostic value EMT-related genes...

Abstract Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) promotes apoptosis of cancer cells by both IGF-dependent and IGF-independent mechanisms. In vitro phosphorylation IGFBP-3 DNA-dependent protein kinase (DNA-PK) has been reported but with unknown functional relevance. Using a chemical inhibitor for DNA-PK in prostate paired system glioblastoma cell lines that either lack or express DNA-PK, we show the apoptosis-promoting growth-inhibitory actions are completely abrogated...

The Journal of Cancer Metastasis and Treatment is an open access journal focused on cancer metastasis treatment, including the occurrence, development, progression, metastasis, treatment oncologic disease. It covers basic, translational clinical research related to cell biology, genomics, precision medicine, oncology internal radiotherapy radiology, obstetrics gynecology, pediatrics, surgery, hematology, neurooncology, etc.

This study aimed to investigate the impact of combined use nuclear factor-κB (NF-κB) inhibitors pyrrolidine dithiocarbamate (PDTC), bortezomib or SN50, and chemotherapy agents arsenic acid (As(2)O(3)), fluorouracil (5FU), oxaliplatin paclitaxel on growth apoptosis HT-29 cells. Cell morphology was observed using inverted microscopy, cell viability were assessed MTT assay flow cytometry, respectively. The activities NF-κB analyzed by western blotting electrophoretic mobility shift (EMSA)....

NPRL2 is a tumor suppressor gene involved in the progression of human cancer. The present study investigated whether expression correlates with colorectal cancer (CRC) progression. Colorectal tissue and peripheral blood samples were obtained from 62 patients CRC, 38 adenomas 51 normal controls. mRNA levels measured using quantitative real-time PCR. protein was determined by immunohistochemistry. CRCs significantly lower than or tissue. decreased compared controls (P<0.0001) it further tumors...

Bortezomib, a first‑in‑class proteasome inhibitor, is standard method of treatment in multiple myeloma. In the present study, therapeutic effect bortezomib was evaluated an ulcerative colitis model induced by dextran sulfate sodium (DSS) mice, and mechanism action also investigated. Mice were administered with 3% DSS drinking water for 7 consecutive days then they intraperitoneally treated (0.2, 0.6 or 1 mg/kg) 1, 3 days. control group provided same volume PBS, respectively. Body weight,...

Accumulating evidence suggested that long non-coding RNAs (lncRNAs) can function as competing endogenous (ceRNAs) to interact with other RNA transcripts and ceRNAs perturbation play important roles in cancer initiation progression including pancreatic adenocarcinoma (PAAD). In this study, we constructed a PAAD-specific hallmark gene-related ceRNA network (HceNet) using paired genome-wide expression profiles of mRNA, lncRNA miRNA regulatory relationships between them. Based on "ceRNA...

Purpose: The study aimed to explore the anticancer effects of a novel proteasome inhibitor, ixazomib, on colorectal cancer (CRC) using combined method microarray and bioinformatics analysis. Materials methods: Cell proliferation was tested by Counting Kit-8 (CCK-8) assay for SW620 cells treated with different concentrations ixazomib treatment times. analysis conducted six samples, including three samples untreated ixazomib. differentially expressed genes (DEGs) between were identified Linear...

5-Fluorouracil (5-FU) is the chemotherapeutic drug of choice for treatment metastatic colorectal cancer (CRC). Tumor suppressor candidate 4 (TUSC4), also referred to as nitrogen permease regulator-like 2 (NPRL2), located at chromosome 3p21.3 and expressed in numerous normal tissues, including heart, liver, skeletal muscle, kidney, pancreas. The aim present study was investigate functional mechanism by which TUSC4 affects sensitivity 5-FU determine its clinical significance CRC. results...

Colon cancer remains a life-threating disease with increasing morbidity and mortality worldwide despite the advancement in modern medical treatment. Therefore, novel effective anti-colon cancers drugs are urgently needed. In this study, we investigated anti-metastatic property EnDuo, modified version of Endostar, underlying mechanisms. cells were treated different concentrations EnDuo (50 μg/mL, 100 200 μg/mL), Endostar (100 μg/mL) as positive control. Cell Counting Kit-8 assay was performed...

The nitrogen permease regulator‑like‑2 (NPRL2) gene is a candidate tumor suppressor gene, which has been identified in the 3p21.3 human chromosome region. Decreased expression levels of NPRL2 have observed colorectal cancer (CRC) tissues, however, function CRC progression remains to be fully elucidated. present study investigated biological characteristics HCT116 and HT29 cell lines overexpressing exogenous NPRL2. recombinant lentiviral vectors were also constructed transfected study. Cell...

Previous studies have suggested that hepatocyte apoptosis may be a fundamental underlying mechanism of liver injury and diseases, such as fibrosis. Relaxin‑3 has been reported to anti‑fibrotic actions in the heart attenuate isoproterenol‑induced myocardial injury; however, beneficial role relaxin‑3 on remains elucidated. The aim present study was explore possible mechanisms through hydrogen peroxide (H2O2)‑induced primary human hepatocytes. Cells were treated with then cell viability,...

Colorectal cancer (CRC) is the third most common worldwide. Chemotherapeutic compounds used for treatment of CRC include oxaliplatin (L-OHP). While L-OHP improves survival, certain patients are resistant. The nitrogen permease regulator like-2 (NPRL2) gene a candidate tumor suppressor that resides in 120-kb homozygous deletion region on chromosome 3p21.3. In present study, it was demonstrated NPRL2 overexpression increases sensitivity HCT116 cells to L-OHP. IC50 decreased transduced with...

BACKGROUND Colorectal cancer (CRC) is the third most common worldwide, making it a serious threat to human health. It imperative develop new therapeutics improve CRC treatment efficiency. The aim of this study was investigate role NPRL2 in improving sensitivity CPT-11 colon cells. MATERIAL AND METHODS overexpression established by transfecting recombinant lentivirus-encoding gene into HCT116 Cell proliferation identified using Counting Kit-8 (CCK8) assay. cycle and apoptosis were examined...