A5048417802- Berry genetics and cultivation research
- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Wheat and Barley Genetics and Pathology
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Plant Physiology and Cultivation Studies
- Horticultural and Viticultural Research
- Powdery Mildew Fungal Diseases
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Yeasts and Rust Fungi Studies
- Plant Reproductive Biology
- Mycotoxins in Agriculture and Food
- Plant and animal studies
- Genetic and phenotypic traits in livestock
- Plant pathogens and resistance mechanisms
- Genetics and Plant Breeding
- Plant Pathogenic Bacteria Studies
- Genetic diversity and population structure
- Garlic and Onion Studies
- Genetic Associations and Epidemiology
- Food Security and Health in Diverse Populations
- Plant Genetic and Mutation Studies
- Plant Gene Expression Analysis
Kansas State University
2022-2023
National Postdoctoral Association
2022
National Clonal Germplasm Repository
2017-2021
United States Department of Agriculture
2017-2020
Agricultural Research Service
2017-2020
Corvallis Environmental Center
2020
North Dakota State University
2014-2019
Oregon State University
2018
University of Arkansas at Fayetteville
2018
Horticultural Crops Research Laboratory
2018
We report an improved assembly and scaffolding of the European pear (Pyrus communis L.) genome (referred to as BartlettDHv2.0), obtained using a combination Pacific Biosciences RSII long-read sequencing, Bionano optical mapping, chromatin interaction capture (Hi-C), genetic mapping. The sample selected for sequencing is double haploid derived from same "Bartlett" reference that was previously sequenced. Sequencing di-haploid plants makes more tractable in highly heterozygous species such P....
Abstract Cultivated strawberry (Fragaria × ananassa) is one of our youngest domesticates, originating in early eighteenth-century Europe from spontaneous hybrids between wild allo-octoploid species chiloensis and Fragaria virginiana). The improvement horticultural traits by 300 years breeding has enabled the global expansion production. Here, we describe genomic history domestication earliest to modern cultivars. We observed a significant increase heterozygosity among interspecific decrease...
The cultivated strawberry (Fragaria×ananassa) is consumed worldwide for its flavor and nutritional benefits. Genetic analysis of commercially important traits in are the development breeding methods tools this species. Although several quantitative trait loci (QTL) have been previously detected fruit quality flowering using low-density genetic maps, clarity on sub-genomic locations these QTLs was missing. Recent discoveries allo-octoploid genomics led to IStraw90 single-nucleotide...
The cultivated strawberry (Fragaria ×ananassa) arose through a hybridization of two wild American octoploid species in French garden the 1750s. Since then, breeders have developed improved cultivars adapted to different growing regions. Diverse germplasm is crucial meet challenges will continue address. USDA-ARS National Clonal Germplasm Repository (NCGR) Corvallis, Oregon maintains U.S. collection. Recent developments high-throughput genotyping for can provide new insights about diversity...
Societal Impact statement To secure a stable, safe food supply, the future of humanity depends on conservation and application cultivated wild plant diversity. Strawberries are globally important. They widely produced provide significant human health benefits. In 2019, annual world production was 14.5 MT (FAOSTAT, 2022). This report characterizes agricultural traits diverse heritage strawberries. The gene pool breeding programs has traditionally included reliable but limited set cultivars....
Rose black spot, caused by Diplocarpon rosae, is one of the most devastating foliar diseases cultivated roses (Rosa spp.). The globally distributed pathogen has potential to cause large economic losses in outdoor cultivation roses. Fungicides are primary method manage disease, but often viewed unfavorably home gardeners due environmental and health impacts. As such, rose cultivars with genetic resistance spot highly desired. tetraploid climbing Brite EyesTM ('RADbrite') known for its spot....
Fire blight, caused by the bacterial pathogen Erwinia amylovora, is a persistent problem for pear (Pyrus spp.) growers in United States. Growing resistant cultivars one of best options managing fire blight. The Potomac and Old Home selection NJA2R59T69 display resistance to As such, three mapping populations (El Dorado × Potomac, Bartlett, Bartlett) were developed identify genomic regions associated with Progeny phenotyped during 2017 2018 inoculating multiple actively growing shoots...
Reliable and high-throughput genotyping platforms are of immense importance for identifying dissecting genomic regions controlling important phenotypes, supporting selection processes in breeding programs, managing wild populations germplasm collections. Amongst available tools, single nucleotide polymorphism arrays have been shown to be comparatively easy use generate highly accurate genotypic data. Single-species the most commonly used type so far; however, some multi-species developed...
Confirming parentage and clonal identity is an important aspect of breeding managing germplasm collections clonally propagated, outcrossing crops, like blackberry ( Rubus subgenus ). DNA fingerprinting sets are used to identify off-cross progeny confirm identity. Previously, a six-simple sequence repeat (6-SSR) set was developed for using small number samples. The usefulness the 6-SSR pedigree confirmation had not been evaluated. Therefore, it in this study validate 6 12 biparental...
Genotyping-by-sequencing (GBS) was used to survey genome-wide single-nucleotide polymorphisms (SNPs) in three biparental strawberry (Fragaria × ananassa) populations with the goal of evaluating this technique a species complex octoploid genome. GBS sequence data were aligned F. vesca 'Fvb' reference genome order call SNPs. Numbers polymorphic SNPs per population ranged from 1,163 3,190. Linkage maps consisting 30-65 linkage groups produced SNP sets derived each parent. The covered 99% Fvb...
Research to identify and characterize stem rust resistance genes in common wheat, Triticum aestivum, has been stimulated by the emergence of Ug99-lineage races wheat pathogen, Puccinia graminis f. sp. tritici (Pgt), Eastern Africa. The Montenegrin spring landrace PI 362698 was identified as a source Pgt resistance. This accession exhibits multiple North American at seedling adult-plant stages. A recombinant inbred population developed crossing susceptible line LMPG-6 with single plant...
The availability of strawberry ( Fragaria × ananassa ) genomic resources has increased dramatically in recent years. Some these are readily applicable to breeding programs for use DNA-informed breeding. Information about tests and how interpret them is dispersed through numerous manuscripts or the laboratories that routinely. To assist breeders identifying available their program implementing program, a compendium DNA was created. This download from Genome Database Rosaceae...
Verification of clonal identity hop ( Humulus lupulus L.) cultivars within breeding programs and germplasm collections is vital to conserving genetic resources. Accurate economic DNA-based tools are needed in dioecious confirm parentage, neither which can be reliably determined from morphological observations. In this study, we developed two fingerprinting sets for hop: a 9-SSR set containing high-core repeats that run single PCR reaction kompetitive allele specific (KASP) assay 25...
Puccinia graminis f. sp. tritici race TTKSF+ was collected from the South African wheat cultivar 'Matlabas' in 2010. F2 and F3 populations derived a Matlabas × Line 37-07 cross segregated for single resistance gene to TTKSF that is avirulent Matlabas. In screening genomic DNA bulks of susceptible or resistant plants with simple sequence repeat (SSR) markers, three chromosome arm 2BS markers one multilocus marker amplified alleles present only Additional 2B-specific SSR incorporating spanning...
ABSTRACT The Lr19 translocation continues to provide broad resistance Puccinia triticina in many parts of the world and can be particularly useful gene pyramids. Previously, an associated for yellow endosperm pigmentation precluded its use countries, as a result, allosyndetic recombinants mutants lacking genes have been developed. Such primary white recombinant ( ‐149) four secondary ‐149–252, −299, −462, −478) were obtained earlier studies. This study characterized translocations making...
The infection process of wheat stem rust (Puccinia graminis f. sp. tritici) on barley (Hordeum vulgare) is often observed as a mesothetic type at the seedling stages, and cultivars containing same major resistance genes show variation in level provided against pathogen race or isolate. Thus, robust phenotyping data based quantification fungal DNA can improve ability to elucidate host-pathogen interaction, especially early time points when disease symptoms are not yet evident. Quantitative...
The necrotrophic fungal pathogen Pyrenophora tritici-repentis (Ptr) causes the foliar disease tan spot in both bread wheat and durum wheat. Wheat lines carrying susceptibility gene Tsc1 are sensitive to Ptr-produced effector (NE) Ptr ToxC. A compatible interaction results leaf chlorosis, reducing yield by decreasing photosynthetic area of leaves. Developing genetically resistant cultivars will effectively reduce incidence. Toward that goal, production chlorosis response inoculation with...