A5014372820- Lipid Membrane Structure and Behavior
- Complement system in diseases
- Escherichia coli research studies
- Advanced Proteomics Techniques and Applications
- Transgenic Plants and Applications
- Mass Spectrometry Techniques and Applications
- Protein purification and stability
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Protein Structure and Dynamics
- Congenital heart defects research
- Neonatal Respiratory Health Research
- Enzyme Catalysis and Immobilization
- Microtubule and mitosis dynamics
- Congenital Diaphragmatic Hernia Studies
- Peptidase Inhibition and Analysis
- Antifungal resistance and susceptibility
- Ubiquitin and proteasome pathways
- Advanced Biosensing Techniques and Applications
- Mindfulness and Compassion Interventions
- Heart Failure Treatment and Management
- Phytochemical and Pharmacological Studies
- Healthcare Education and Workforce Issues
Oak Ridge National Laboratory
2017-2020
University of Antwerp
2014-2020
Innsbruck Medical University
2017-2020
Universität Innsbruck
2017-2020
University of Tennessee at Knoxville
2017
Vanderbilt University
2013
Examining the fundamental structure and processes of living cells at nanoscale poses a unique analytical challenge, as are dynamic, chemically diverse, fragile. A case in point is cell membrane, which too small to be seen directly with optical microscopy provides little observational contrast for other methods. As consequence, characterization membrane has been performed ex vivo or presence exogenous labels used enhance impart specificity. Here, we introduce an isotopic labeling strategy...
The Consortium for Top-Down Proteomics (www.topdownproteomics.org) launched the present study to assess current state of top-down mass spectrometry (TD MS) and middle-down (MD characterizing monoclonal antibody (mAb) primary structures, including their modifications. To meet needs rapidly growing therapeutic market, it is important develop analytical strategies characterize heterogeneity a product's structure accurately reproducibly. major objective determine whether TD/MD MS technologies...
Organ development is a highly regulated process involving the coordinated proliferation and differentiation of diverse cellular populations. The pathways regulating cell their effects on organ growth are complex for many organs incompletely understood. In all vertebrate species, cardiac natriuretic peptides (ANP BNP) produced by cardiomyocytes in developing heart. However, role during cardiogenesis not defined. Using embryonic zebrafish neonatal mammalian we explored peptide signaling...
Lipid extracts are an excellent choice of model biomembrane; however at present, there no commercially available lipid or computational models that mimic microbial membranes containing the branched-chain fatty acids found in many pathogenic and industrially relevant bacteria. We advance extract Bacillus subtilis as a standard for these diverse systems, providing detailed experimental description equilibrated atomistic bilayer included Supporting Information to this Letter...
A novel antibody–tetrazine conjugate was utilized for live-cell imaging together with TCO probes. SKOV-3 cells were selectively labelled.
Abstract Aurora kinases B and C (AURKB/AURKC) are activated by binding to the C-terminal domain of INCENP. Full activation requires phosphorylation two serine residues INCENP that conserved through evolution, although mechanism this has not been explained. Here we present crystal structures fully active complex AURKC bound INCENP, consisting phosphorylated, activated, phosphorylated on its TSS motif, revealing structural biochemical synergistic AURKC:INCENP. The show motif stabilises kinase...
Complement is a tightly controlled arm of the innate immune system, facilitating phagocytosis and killing invading pathogens. Factor H (FH) main fluid-phase inhibitor alternative pathway. Many pathogens can hijack FH from host protect themselves complement-dependent killing. Candida albicans clinically important opportunistic yeast, expressing different binding molecules on its cell surface, which allow complement evasion. One such molecule transmembrane protein "High affinity glucose...
Hemolytic uremic syndrome (eHUS) is a severe complication of human infections with Shiga toxins (Stxs)-producing Escherichia coli. A key step in the pathogenesis eHUS interaction Stxs blood components before targeting renal endothelial cells. Here, we show that single proteolytic cleavage Stx2a A-subunit, resulting into two fragments (A1 and A2) linked by disulfide bridge (cleaved Stx2a), dictates different binding abilities. Uncleaved was confirmed to bind neutrophils trigger...
Developing cultivation methods that yield chemically and isotopically defined fatty acid (FA) compositions within bacterial cytoplasmic membranes establishes an in vivo experimental platform to study membrane biophysics cell regulation using novel approaches. Yet before fully realizing the potential of this method, it is prudent understand systemic changes cells induced by labeling procedure itself. In work, analysis cellular was paired with shotgun proteomics assess how proteome response...
Abstract The identification of peptides and proteins from tandem mass spectra is a difficult task multiple tools have been developed to aid this identification. We present new method called quantum chemical spectrometry for materials science (QCMS 2 ), which based on calculations bond orders, reaction, transition‐state energies at the DFT/B3LYP/6‐311+G* level theory. was used describe fragmentation pathways five X‐His‐Ser tripeptides with X = Asn, Asp, Glu, Ser, Trp, thereby focusing...
Enterohemorrhagic Escherichia coli (EHEC) infections can cause EHEC-associated hemolytic uremic syndrome (eHUS) via its main virulent factor, Shiga toxins (Stxs). Complement has been reported to be involved in the progression of eHUS. The aim this study was investigate interactions most effective subtype toxin, Stx2a, with pivotal complement proteins C3b and C5. further examined effect Stx2a stimulation on transcription synthesis these human target cell lines. Binding C5 evaluated by ELISA....