Marietta L Lagrada
A5017221947- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Infections and Vaccines
- Listeria monocytogenes in Food Safety
- Plant Pathogenic Bacteria Studies
- Biochemical and Structural Characterization
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Bacteriophages and microbial interactions
- Healthcare Systems and Reforms
- Health Systems, Economic Evaluations, Quality of Life
- Plant Pathogens and Fungal Diseases
- Genetics, Bioinformatics, and Biomedical Research
- Plant pathogens and resistance mechanisms
Research Institute for Tropical Medicine
2017-2023
Department of Health
2021-2023
CNR de la Résistance aux Antibiotiques
2009
Abstract Background Klebsiella species, including the notable pathogen K. pneumoniae, are increasingly associated with antimicrobial resistance (AMR). Genome-based surveillance can inform interventions aimed at controlling AMR. However, its widespread implementation requires tools to streamline bioinformatic analyses and public health reporting. Methods We developed web application Pathogenwatch, which implements analytics tailored species for integration visualization of genomic...
Abstract National networks of laboratory-based surveillance antimicrobial resistance (AMR) monitor trends and disseminate these data to AMR stakeholders. Whole-genome sequencing (WGS) can support by pinpointing mechanisms uncovering transmission patterns. However, genomic is rare in low- middle-income countries. Here, we implement WGS within the established Antimicrobial Resistance Surveillance Program Philippines via a binational collaboration. In parallel, characterize bacterial...
Abstract Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a threat to public health in India because of its high dissemination, mortality, and limited treatment options. Its genomic variability reflected the diversity sequence types, virulence factors, antimicrobial resistance (AMR) mechanisms. This study aims characterize clonal relationships genetic mechanisms CRKP isolates India. Materials Methods We characterized 344 retrospective K. clinical collected from 8 centers...
Abstract Background Klebsiella pneumoniae is a World Health Organization high-priority antibiotic-resistant pathogen. However, little known about lineages circulating in Nigeria. Methods We performed whole-genome sequencing (WGS) of 141 isolated between 2016 and 2018 from clinical specimens at 3 antimicrobial-resistance (AMR) sentinel surveillance tertiary hospitals southwestern conducted silico multilocus sequence typing; AMR gene, virulence plasmid, K O loci profiling; as well phylogenetic...
Abstract Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an emerging public health problem. This study explores the specifics of CRKP epidemiology in Colombia based on whole genome sequencing (WGS) National Reference Laboratory at Instituto Nacional de Salud (INS)’s 2013–2017 sample collection. Methods A total 425 isolates from 21 departments were analyzed by HiSeq-X10®Illumina high-throughput sequencing. Bioinformatic analysis was performed, primarily using pipelines...
Abstract Objectives Doxycycline post-exposure prophylaxis (doxycycline-PEP) can reduce incident cases of syphilis, chlamydia and possibly gonorrhoea especially among men who have sex with recent bacterial sexually transmitted infections (STIs). Owing to potential implementation doxycycline-PEP internationally, global tetracycline/doxycycline resistance data for contemporary Neisseria gonorrhoeae isolates has become imperative. We report tetracycline gonococcal (n = 2993) from eight WHO...
Abstract Antimicrobial resistance (AMR) is considered a global threat, and novel drug discovery needs to be complemented with systematic standardized epidemiological surveillance. Surveillance data are currently generated using phenotypic characterization. However, due poor scalability, this approach does little for true investigations. There strong case whole-genome sequencing (WGS) enhance the data. To establish AMR surveillance WGS, we developed laboratory implementation that applied...
Klebsiella pneumoniae is a critically important pathogen in the Philippines. Isolates are commonly resistant to at least 2 classes of antibiotics, yet mechanisms and spread its resistance not well studied.A retrospective sequencing survey was performed on carbapenem-, extended spectrum beta-lactam-, cephalosporin-resistant isolated 20 antimicrobial (AMR) surveillance sentinel sites from 2015 through 2017. We characterized 259 isolates using biochemical methods, susceptibility testing,...
Abstract Performing whole genome sequencing (WGS) for the surveillance of antimicrobial resistance offers ability to determine not only antimicrobials which rates are increasing, but also evolutionary mechanisms and transmission routes responsible increase at local, national, global scales. To derive WGS-based outputs, a series processes required, beginning with sample metadata collection, followed by nucleic acid extraction, library preparation, sequencing, analysis. Throughout this pathway...
Recent data on the burden of hospitalization and clinic visits for rotavirus gastroenteritis are needed to support decision introduce vaccine in Philippines.From 2005 through 2006, children aged <5 years with acute diarrhea who attended 1 7 clinics and/or hospitals Muntinlupa City, Philippines, were enrolled. Clinical demographic collected, a stool specimen was obtained testing typing G P antigens. The incidences different clinical outcomes determined 3 townships under surveillance...
Abstract Advanced genomics and sequencing technologies are increasingly becoming critical for global health applications such as pathogen antimicrobial resistance (AMR) surveillance. Limited resources challenge capacity development in low- middle-income countries (LMICs), with few having facilities adequately trained staff. Training research public experts who directly involved the establishment of offers an effective, but limited, solution to a growing need. Instead, training them impart...
Pseudomonas aeruginosa is an opportunistic pathogen that often causes nosocomial infections resistant to treatment. Rates of antimicrobial resistance (AMR) are increasing, as rates multidrug-resistant (MDR) and possible extensively drug-resistant (XDR) infections. Our objective was characterize the molecular epidemiology AMR mechanisms this pathogen. We sequenced whole genome for each 176 P. isolates collected in Philippines 2013-2014; derived multilocus sequence type (MLST), presence...
In this Supplement, we detail outputs of the National Institute for Health Research Global Unit on Genomic Surveillance Antimicrobial Resistance project, covering practical implementation whole-genome sequencing across our consortium, which consists laboratories in Colombia, India, Nigeria, and Philippines.
Methicillin-resistant Staphylococcus aureus (MRSA) remains one of the leading causes both nosocomial and community infections worldwide. In Philippines, MRSA rates have remained above 50% since 2010, but resistance to other antibiotics, including vancomycin, is low. The burden can be partially attributed pathogen-specific characteristics circulating clones, little was known about S. clones in Philippines. We sequenced whole genomes 116 isolates collected 2013–2014 within Antimicrobial...
Abstract Background Increasing antimicrobial resistance (AMR) in Salmonella has been observed the Philippines. We aimed to characterise population and AMR mechanisms of with whole genome sequencing (WGS) compare it laboratory surveillance methods. Methods The serotype, multilocus sequence type, genes relatedness between isolates were determined from genomes 148 Typhi (S. Typhi) 65 non-typhoidal (NTS) collected by Antimicrobial Resistance Surveillance Program during 2013–2014. Genotypic...
Abstract Drug-resistant bacterial infections constitute a growing threat to public health globally 1 . National networks of laboratory-based surveillance antimicrobial resistance (AMR) monitor the emergence and spread are central dissemination these data AMR stakeholders 2 Whole-genome sequencing (WGS) can support efforts by pinpointing mechanisms uncovering transmission patterns 3, 4 However, genomic is rare in low- middle-income countries (LMICs), which predicted be most affected 5 We...
Abstract Antimicrobial resistance (AMR) is considered a global threat, and novel drug discovery needs to be complemented with systematic standardized epidemiological surveillance. Surveillance data are currently generated using phenotypic characterization. However, due poor scalability, this approach does little for true investigations. There strong case whole-genome sequencing (WGS) enhance the data. To establish AMR surveillance WGS, we developed laboratory implementation that applied...
The administration and governance of grant funding across global health organizations presents enormous challenges. Meeting these challenges is crucial to ensuring that funds are used in the most effective way improve outcomes, line with United Nations' Sustainable Development Goal 3, "Ensure healthy lives promote well-being for all at ages." Good Financial Grant Practice (GFGP) Standard (ARS 1651) world's first and, currently, only international standard financial management funding....
Antimicrobial-resistant Neisseria gonorrhoeae is a major threat to public health and of particular concern in the Western Pacific Region, where incidence gonorrhoea high. The Antimicrobial Resistance Surveillance Program (ARSP) has been capturing information on resistant since 1996, but genomic epidemiology studies this pathogen are lacking Philippines. We sequenced whole genomes 21 N. isolates collected 2013–2014 by ARSP. multilocus sequence type, multiantigen presence determinants...
Objective: Acinetobacter baumannii is an opportunistic nosocomial pathogen that has increasingly become resistant to carbapenems worldwide. In the Philippines, rates of carbapenem resistance and multidrug are above 50%. We undertook a genomic study carbapenem-resistant A. in Philippines characterize population diversity antimicrobial mechanisms. Methods: sequenced whole genomes 117 isolates recovered by 16 hospitals between 2013 2014. From genome sequences, we determined multilocus sequence...
Objective: The epidemiology of invasive non-typhoidal Salmonella (iNTS) in the Philippines is not well elaborated. present study describes serotype distribution and antimicrobial susceptibility patterns iNTS from 2014 to 2018. Methods: Invasive NTS isolates were collected through Department Health’s Antimicrobial Resistance Surveillance Program (ARSP). identification was confirmed using automated (Vitek®, bioMérieux, Marcy l’Étoile, France) conventional methods. serotyped slide agglutination...
Abstract Methicillin Resistant Staphylococcus aureus (MRSA) remains one of the leading causes both nosocomial and community infections worldwide. In Philippines, MRSA rates have remained above 50% since 2010, but resistance to other antibiotics, including vancomycin, is low. The burden can be partially attributed pathogen-specific characteristics circulating clones, little was known about S. clones in Philippines. We sequenced whole genomes 116 isolates collected 2013-2014 by Antimicrobial...
ABSTRACT Background Klebsiella pneumoniae is a critically important pathogen in the Philippines. Isolates are commonly resistant to at least two classes of antibiotics, yet mechanisms and spread its resistance not well studied. Methods A retrospective sequencing survey was performed on carbapenem-, extended spectrum beta-lactam- cephalosporin-resistant isolated 20 antimicrobial (AMR) surveillance sentinel sites from 2015-2017. We characterized 259 isolates using biochemical methods,...
Objective: Data are scarce on the prevailing Streptococcus pneumoniae serotypes in Philippines, including relative antimicrobial resistance (AMR) of these bacteria. This study is designed to fill that gap by describing serotype distribution and AMR S. Philippines from 2012 2018. Methodology: isolates clinical specimens were collected through Philippine Department Health Antimicrobial Resistance Surveillance Program 1 January 31 December Identification susceptibility testing (AST) performed...