Abstract Development of reporter genes for multimodality molecular imaging is highly important. In contrast to the conventional strategies which have focused on fusing several together serve as multimodal reporters, human tyrosinase (TYR) – key enzyme in melanin production was evaluated this study a stand-alone gene vitro and vivo photoacoustic (PAI), magnetic resonance (MRI) positron emission tomography (PET). Human breast cancer cells MCF-7 transfected with plasmid that encodes TYR (named...

Elastic metasurfaces have become one of the most promising platforms for manipulating mechanical wavefronts with striking feature ultra-thin geometry. The conventional design significantly relies on numerical, trial-and-error methods to identify structural parameters unit cells, which requires huge computational resources and could be extremely challenging if metasurface is multi-functional. Machine learning technique provides another powerful tool multi-functional elastic because its...

Analog spatial differentiation is used to realize edge-based enhancement, which plays an important role in data compression, microscopy, and computer vision applications. Here, a planar chip made from dielectric multilayers proposed operate as both first- second-order differentiator without any need change the structural parameters. Third- fourth-order differentiations that have never been realized before, are also experimentally demonstrated with this chip. A theoretical analysis explain...

Fluorescence microscopy offers high sensitivity for disease diagnosis. However, little structural information is revealed by this method, requiring another technique to localize the source of fluorescence. We developed a complementary fluorescence-polarization microscope. used division-of-focal-plane charge coupled device polarization sensor enable real-time video rate imaging without any moving parts. The provided microscope enabled detection element and complements fluorescence...

We performed an experiment to observe the storages of input probe field and idler generated through off-axis four-wave mixing (FWM) process via a double-lambda configuration in cold atomic ensemble. analyzed underlying physics detail found that retrieved came from two parts if there was no single-photon detuning for pump pulse: part 1 collective spin (the field, coupling combined generate idle FWM, then stored electromagnetically induced transparency.); 2 new FWM during retrieval signal). If...

The pistons of sparse aperture systems need to be controlled within a fraction wavelength for the system's optimal imaging performance. In this paper, we demonstrate that deep learning is capable performing piston sensing with single wide-band image after appropriate training. Taking issue as fitting task, learning-based method utilizes convolutional neural network learn complex input-output mapping relations between broadband intensity distributions and corresponding values. Given trained...

Nanoparticle based image-guided therapy is an emerging technology for cancer in recent years. Here, we report simultaneous photoacoustic (PA)- and magnetic resonance (MR)-guided photothermal ablation (PTA) using multifunctional superparamagnetic iron oxide-containing gold nanoshells (SPIO@AuNS). Based on the intrinsic high near-infrared optical absorbance strong property of SPIO@AuNS, carried out vivo dual-modality PA-MR imaging mouse tumors. PA- MR-guided can monitor therapeutic effect...

Nanoparticles have become a powerful tool for cell imaging and biomolecule, protein interaction studies, but are difficult to rapidly accurately measure in most assays. Dark-field microscope (DFM) image analysis approaches used quantify nanoparticles require high-magnification near-field (HN) images that labor intensive due requirement manual selection focal adjustments needed when identifying capturing new regions of interest. Low-magnification far-field (LF) DFM imagery is technically...

Dielectric multilayer photonic-band-gap structures, called one-dimensional photonic crystals (1DPCs), have drawn considerable attention in the fields of physics, chemistry, and biophotonics. Here, experimental results verify feasibility a 1DPC working as substrate for switchable manipulations colloidal microparticles. The optically induced thermal convective force on can assemble particles that are dispersed water solution, while scattering same caused by propagating evanescent waves guide...

A steady optical beam (SOB) propagating stably in a disorder medium is constructed by using specially designed aspherical lens. Our theoretical and experimental results show that the generated SOB exhibits much better propagation features with small divergence long Rayleigh length, as well weak deformation through turbulent environment compared conventional Gaussian beam. The parameter product of reaches 49.40% multiple measurements within certain distance range. may find applications...

Recently, tremendous remarkable studies on spin–orbit interactions (SOIs) have attracted interest of scientists across the world in various fields. Among contemporary technologies probing SOI, photonic metasurfaces become a prevalent tool to guide and steer wavefront light at subwavelength scale. Nevertheless, discretized nanoantennas been plagued for shortcoming bandwidth. In contrast strategy, symmetric nanorings possessing continuous phase gradient are exploited this work. Benefiting from...

Abstract. The hygroscopic growth of atmospheric aerosols plays an important role in regional radiation, cloud formation, and hence climate. Aerosol is often characterized by tandem differential mobility analyzers (HTDMAs), Xie et al. (2020) recently demonstrated that measurements a single particle are possible using surface plasmon resonance microscope-azimuthal rotation illumination (SPRM-ARI). properties ambient not uniform exhibit large relative humidity (RH) size variabilities due to...

Label-free optical imaging of single-nanometer-scale matter is extremely important for a variety biomedical, physical, and chemical investigations. One central challenge that the background intensity much stronger than scattering light from single nano-objects. Here, we propose an module comprising cascaded momentum-space polarization filters can perform vector field modulation to block most result in almost black background; contrast, only small proportion blocked, leading obvious contrast...

Nanotechnology provides tremendous biomedical opportunities for cancer diagnosis, imaging, and therapy. In contrast to conventional chemotherapeutic agents where their actual target delivery cannot be easily imaged, integrating imaging therapeutic properties into one platform facilitates the understanding of pharmacokinetic profiles, enables monitoring process in each individual. Such a concept dubbed "theranostics" potentiates translational research improves precision medicine. One...

Monitoring dynamic interactions of T cells migrating toward tumor is beneficial to understand how cancer immunotherapy works. Optical-resolution photoacoustic microscope (OR-PAM) can provide not only high spatial resolution but also deeper penetration than conventional optical microscopy. With the aid exogenous contrast agents, dual-wavelength OR-PAM be applied map distribution CD8+ cytotoxic lymphocytes (CTLs) with gold nanospheres (AuNS) under 523nm laser irradiation and Hepta1-6 spheres...

Surface plasmon resonance microscopy (SPRM) has proved to be a powerful tool for label‐free imaging, detection, and mass/size measurement of single isolated virus, cells, nanoparticles. Herein, SPRM that can extended detect the dense nanoparticles, such as counting number density is demonstrated. To improve accuracy, multiangle illumination adopted in conventional SPRM, then deep learning method based on pretrained neural network approach proposed deal with large images. Numerical...

The measurements of the temperature field are very important and difficult for generally single-point measuring instrument. Here we presented a simple sensor by using chirped fiber Bragg grating (CFBG) as sensing element. As varies around CFBG along optical Z-axis, because photo-thermal effect, change in gradient will lead to shifting CFBG's spectrum. We believe that this configuration open way research CFBG-derived theories configurations detection fields.

Abstract Single-particle tracking (SPT) is an immensely valuable technique to study a variety of processes in the life sciences and condensed matter physics. Interferometric scattering (iSCAT) microscopy sensitive SPT that can track individual unlabeled particles with high spatial temporal resolution. A difficulty iSCAT low imaging contrast its original image, complicated postprocessing method necessary for deriving axial-location particle. Here, planar photonic chip enhanced spin-to-orbital...

Abstract Analog spatial differentiation is used to realize edge-based enhancement, which plays an important role in data compression, microscopy, and computer vision applications. Here, a planar chip made from dielectric multilayers proposed operate as both first- second-order differentiator without any need change the structural parameters. Third- fourth-order differentiations that have never been realized before, are also experimentally demonstrated with this chip. A theoretical analysis...