A5027583042- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Axon Guidance and Neuronal Signaling
- Angiogenesis and VEGF in Cancer
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Cancer, Hypoxia, and Metabolism
- Dental Health and Care Utilization
- Cancer-related gene regulation
- Neurogenesis and neuroplasticity mechanisms
- Hedgehog Signaling Pathway Studies
- Cannabis and Cannabinoid Research
- Health Policy Implementation Science
- Corneal Surgery and Treatments
- Wnt/β-catenin signaling in development and cancer
- Oral Health Pathology and Treatment
- Endodontics and Root Canal Treatments
- Mobile Health and mHealth Applications
- Metabolism, Diabetes, and Cancer
- Ocular Surface and Contact Lens
- Fibroblast Growth Factor Research
- Wound Healing and Treatments
- MicroRNA in disease regulation
Anhui Medical University
2021-2025
University of Hong Kong
2002-2019
Prince Philip Dental Hospital
2018
Queen Mary Hospital
2002
Functional reconstruction of maxillofacial bone defects is a considerable clinical challenge. Many studies have emphasized the osteogenic and angiopoietic abilities stem cells for tissue regeneration. We previously showed that microRNA-21 (miRNA-21) can promote angiogenesis in human umbilical cord blood-derived mesenchymal (UCBMSCs). In present study, role miRNA-21 differentiation marrow-derived (BMSCs) was investigated. Western blotting qPCR were performed to investigate influences on...
The synchronized development of mineralized bone and blood vessels is a fundamental requirement for successful tissue regeneration. Adequate energy production forms the cornerstone supporting new formation. ETS variant 2 (ETV2) has been identified as transcription factor that promotes metabolism reprogramming facilitates coordination between osteogenesis angiogenesis. In vitro molecular experiments have demonstrated ETV2 enhances osteogenic differentiation dental pulp stem cells (DPSCs) by...
The aging of bone marrow mesenchymal stem cells (BMSCs) impairs tissue regeneration, contributing to skeletal disorders. LncRNA NEAT1 is considered as a proliferative inhibitory role during cellular senescence, but the relevant mechanisms remain insufficient. This study aims elucidate how regulates mitotic proteins BMSCs aging. were isolated from alveolar human volunteers aged 26–33 (young) and 66–78 (aged). expression distribution changes process observed using fluorescence in situ...
Abstract Background The repair of cranio-maxillofacial bone defects remains a formidable clinical challenge. Ets variant 2 (ETV2) transcription factor, which belongs to the E26 transformation-specific (ETS) family, has been reported play key role in neovascularization. However, ETV2 osteogenesis human dental pulp stem cells (hDPSCs) unexplored. Methods Transgenic overexpression was achieved using lentiviral vector, based on Dox-inducible system. effects Dox-induced hDPSCs were evaluated by...
Abstract Dental stem cells can serve as a potential source of functional endothelial for tissue engineering applications, but the endothelial‐lineage differentiation efficiency is rather low even with growth factors and mechanical stimuli, which greatly limits their clinical applications. This partly due to deficiency standard two‐dimensional (2‐D) culture systems, unable recapitulate three‐dimensional (3‐D) in vivo milieu that rich extracellular matrix. Hence, we extracted decellularized...
Low efficiency of deriving endothelial cells (ECs) from adult stem hampers their utilization in tissue engineering studies. The purpose this study was to investigate whether suppression transforming growth factor beta (TGF-β) signaling could enhance the differentiation dental pulp–derived into ECs. We initially used vascular A (VEGF-A) stimulate 2 (dental pulp and human exfoliated deciduous teeth [SHED]) compared capacity further evaluated receptor I (VEGF-RI)-specific ligand placental...
This randomized controlled trial aimed to evaluate the effectiveness of an online application based on HAPA theory oral hygiene promotion in young adults with fixed orthodontic appliances. A WeChat mini-program (called "Clean Teeth") was designed beforehand improve oral-health behaviors and hygiene, 44 participants aged 17-29 appliances were recruited. Participants experimental group (n = 22) received Teeth" mini-program, addition care as usual, control only routine health education. Data...
The lack of vasculogenesis often hampers the survivability and integration newly engineered tissue grafts within host. Autologous endothelial cells (ECs) are an ideal cell source for neovascularization, but they limited by their scarcity, proliferative capacity, donor site morbidity upon isolation. objective this study was to determine whether differentiation human dental pulp stem (DPSCs) into lineage can be enhanced recombinant ETV2 overexpression. DPSCs were extracted from fresh tissues....
Aim Occlusion dysfunction (OD) is increasingly linked to Alzheimer’s disease (AD). This study aimed elucidate the causal relationship between OD and AD using Mendelian randomization (MR) analysis. Materials methods Genome-wide association (GWAS) meta-analysis data obtained from FinnGen, IEU Open GWAS, UK Biobank (UKBB) was represented as instrumental variables. We validated periodontal (PD), loose teeth (PD & occlusion dysfunction), dentures restoration (occlusion recovery), AD....
Effects of shear stress on endotheliaxl differentiation stem cells from human exfoliated deciduous teeth (SHEDs) were investigated. SHEDs treated with stress, then reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to analyse the mRNA expression arterial markers and western blot analysis protein angiogenic markers. Additionally, in vitro matrigel angiogenesis assay evaluate vascular-like structure formation. The secreted levels vascular endothelial growth...
Abstract Inducing of dental pulp stem cells (DPSCs) into endothelial (ECs) to prevascularize tissue constructs may offer a novel and viable approach for enhancing regeneration. However, there are numerous challenges in current methods the acquisition sufficient translational ECs. It was known that Sema4D/PlexinB1 signaling exerts profound effects on vascular growth factor (VEGF) secretion angiogenesis. Whether could regulate differentiation DPSCs is not yet investigated. In this study, when...
Calcium silicate cements have been widely used for pulpotomies in immature permanent teeth with complicated crown fractures due to their superior properties. However, few studies evaluated the long-term outcomes of white mineral trioxide aggregate (WMTA) and iRoot BP Plus partial pulpotomies. The aim this study was investigate clinical radiographic WMTA incisors fractures.Children who had using or as capping agents were enrolled. Eighty 68 children (aged 8-13 years) included. They divided...
Wnt4 has been shown to promote the recovery of odontogenic differentiation dental pulp stem cells under inflammatory conditions, but its role in inflammation and apoptosis pulpitis remains be elucidated. Lipopolysaccharide (LPS) (10 µg/ml) was applied treat human (HDPCs) for 24 h. Western blotting measured expressions cytokines apoptosis-related proteins. Cell by flow cytometry. The level evaluated reverse transcription-quantitative PCR western blotting. results indicated that LPS could...
Dental pulp stem cells (DPSCs) originate from the embryonic neural crest and have neurogenic potential. The present study investigated roles of forward reverse EphrinB2 signalling pathways during DPSC neurogenesis. Treatment DPSCs with recombinant EphrinB2‑Fc protein over 7 days in a induction culture resulted significant downregulation following markers: βIII‑Tubulin, cell adhesion molecule (NCAM), nestin, neurogenin 2 (NGN2), neurofilament medium polypeptide Musashi1. Immunocytochemistry...
Extensive bone fractures, which can seriously impact both health and quality of life, cannot easily heal naturally, especially if the patient has an underlying medical condition or is aging. The most promising approach to addressing such fractures regeneration through tissue engineering. Bone a complex process that consists three distinct phases: inflammation, repair, remodeling. Macrophages play bridging role between various cells involved in each stage regeneration, interacting with...
Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) for the treatment of bone defects have been widely reported as a cell-free therapy because their appropriate stability and biocompatibility. However, EV isolation is expensive time-consuming. We developed method modifying EVs marrow MSCs (BMSCs) via cationic polymer (ERP) with characteristics charge reversal esterase response (ERP-EVs). When simply mixing BMSCs-EVs ERP at 1:1 ratio, ERP-EVs significantly enhanced...