A5078812198- Reading and Literacy Development
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracranial Aneurysms: Treatment and Complications
- Microbial bioremediation and biosurfactants
- Winter Sports Injuries and Performance
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Text Readability and Simplification
- Intracerebral and Subarachnoid Hemorrhage Research
- Neurobiology of Language and Bilingualism
- Neuroscience and Music Perception
- Nerve Injury and Rehabilitation
- Education Methods and Technologies
- Visual perception and processing mechanisms
- Shoulder and Clavicle Injuries
- Shoulder Injury and Treatment
- Multisensory perception and integration
- Linguistic research and analysis
- Spinal Cord Injury Research
- Biofuel production and bioconversion
- Congenital Heart Disease Studies
- Child Development and Digital Technology
- Language Development and Disorders
- Linguistic Education and Pedagogy
- Neural and Behavioral Psychology Studies
University of Zurich
2014-2023
University Hospital of Zurich
2019-2023
Pädagogische Hochschule Bern
2021-2023
University of Fribourg
2020
University Hospital of Basel
2015-2018
University of Basel
2018
Kantonsspital Aarau
2015
ETH Zurich
1999-2014
University Hospital of Bern
1986-2014
University of Bern
2014
ABSTRACT Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show most of these enzymes related to CYP153A1 CYP153A6, cytochrome P450 were characterized as A vector for the polycistronic coexpression individual CYP153 genes with a ferredoxin gene reductase was constructed. Seven 11 tested allowed Pseudomonas putida GPo12 recombinants well alkanes,...
The Pseudomonas putida GPo1 (commonly known as oleovorans GPo1) alkBFGHJKL and alkST gene clusters, which encode proteins involved in the conversion of n-alkanes to fatty acids, are located end on OCT plasmid, separated by 9·7 kb DNA. This DNA segment encodes, amongst others, a methyl-accepting transducer protein (AlkN) that may be chemotaxis alkanes. In P. P1, clusters flanked almost identical copies insertion sequence ISPpu4, constituting class 1 transposon. Other sequences flank interrupt...
We have developed highly degenerate oligonucleotides for polymerase chain reaction (PCR) amplification of genes related to the Pseudomonas oleovorans GPo1 and Acinetobacter sp. ADP1 alkane hydroxylases, based on a number conserved sequence motifs. In all Gram‐negative in two out three Gram‐positive strains able grow medium‐ (C 6 –C 11 ) or long‐chain n ‐alkanes 12 16 ), PCR products expected size were obtained. The fragments cloned sequenced found encode peptides with 43.2–93.8% identity...
ABSTRACT Pseudomonas sp. VLB120 uses styrene as a sole source of carbon and energy. The first step in this metabolic pathway is catalyzed by an oxygenase (StyA) NADH-flavin oxidoreductase (StyB). Both components have been isolated from wild-type strain well recombinant Escherichia coli . StyA both sources dimer, with subunit size 47 kDa, catalyzes the enantioselective epoxidation C═C double bonds. Styrene exclusively converted to S -styrene oxide specific activity 2.1 U mg −1 ( k cat = 1.6 s...
Anatomical plasticity such as fibre growth and the formation of new connections in cortex spinal cord is one known mechanism mediating functional recovery after damage to central nervous system. Little about anatomical brainstem, which contains key locomotor regions. We compared changes projection pattern major descending systems following a cervical unilateral hemisection adult rats. As humans (Brown-Séquard syndrome), this type injury resulted permanent loss fine motor control ipsilesional...
Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium each one homologue, however there is no evidence for presence in remaining strains.
Selection experiments and protein engineering were used to identify an amino acid position in integral membrane alkane hydroxylases (AHs) that determines whether long-chain-length alkanes can be hydroxylated by these enzymes. First, substrate range mutants of the Pseudomonas putida GPo1 Alcanivorax borkumensis AP1 medium-chain-length AHs obtained selection with a specially constructed host. In all able oxidize longer than C13, W55 (in case P. AlkB) or W58 A. AlkB1) had changed much less...
Summary The marine γ‐Proteobacterium Alcanivorax borkumensis is highly specialized in the assimilation of aliphatic hydrocarbons, and makes up a large part biomass oil‐polluted environments. In addition to previously identified alkane hydroxylase AlkB1, second (AlkB2) showing 65% identity Pseudomonas aeruginosa AlkB2 was identified. Unlike alkB1 , alkB2 not flanked by genes involved metabolism. Heterologous expression A. AP1 showed that they encode functional hydroxylases with substrate...
Learning letter-speech sound correspondences is a major step in reading acquisition and severely impaired children with dyslexia. Up to now, it remains largely unknown how quickly neural networks adopt specific functions during audiovisual integration of linguistic information when prereading learn correspondences. Here, we simulated the process learning 20 (6.13-7.17 years) at varying risk for dyslexia by training artificial within single experimental session. Subsequently, acquired...
Abstract During reading acquisition, neural reorganization of the human brain facilitates integration letters and speech sounds, which enables successful reading. Neuroimaging behavioural studies have established that impaired audiovisual sounds is a core deficit in individuals with developmental dyslexia. This longitudinal study aimed to identify markers are related future fluency. We simulated first step acquisition by performing artificial-letter training prereading children at risk for...
The ventral occipitotemporal (vOT) cortex serves as a core region for visual processing, and specific areas of this show preferential activation various categories such faces print. emergence functional specialization in the human represents pivotal developmental process, which provides basis targeted efficient information processing. For example, to print left vOT is an important prerequisite fluent reading. However, it remains unclear, processes initiate cortical activations characters...
The level of reading skills in children and adults is reflected the strength preferential neural activation to print. Such appears N1 event-related potential (ERP) over occipitotemporal scalp after around 150-250 ms corresponding blood oxygen dependent (BOLD) signal ventral (vOT) cortex. Here, orthography-sensitive (print vs. false font) processing was examined using simultaneous EEG-fMRI 38 first grade with poor typical skills, at varying familial risk for developmental dyslexia. Coarse...
The substrate reactivity of the flavoenzyme 2-hydroxybiphenyl 3-monooxygenase (EC 1.14.13.44, HbpA) was changed by directed evolution using error-prone PCR. <i>In situ</i> screening mutant libraries resulted in identification proteins with increased activity towards 2-<i>tert</i>-butylphenol and guaiacol (2-methoxyphenol). One enzyme variant contained amino acid substitutions V368A/L417F, which were inserted two rounds mutagenesis. double replacement improved efficiency hydroxylation...
We assessed the Swiss-German version of GraphoLearn, a computer game designed to support reading by training grapheme-phoneme correspondences. A group 34 children at risk for dyslexia trained three times week during 14 weeks, on top their standard school instruction. The sample was divided into two groups 18 and 16 children, who started either middle or end first grade. found beneficial effects in pseudoword both rapid automatized naming skills that earlier. Our results suggest efficiency...
Zusammenfassung Kinder mit Deutsch als Zweitsprache zeigen im Vergleich zu monolingualen, deutschsprachigen Kindern eklatante Defizite beim Lesen, so auch in der vorliegenden Studie. Untersucht wurde die Rolle verschiedener Wortschatzaspekte an Zweitklässlern Erst- ( N = 351) und 54). Der Effekt des Faktors Wortschatz auf das Lesen erwies sich insgesamt signifikant. Wenn kontrolliert wurde, zeigte kein Leistungsgefälle mehr zwischen den Gruppen. Die auffällige relationalen Wortwissens für...
Abstract The Visual Word Form Area (VWFA) is a key region of the brain’s reading network and its activation has been shown to be strongly associated with skills. Here, for first time, we investigated whether voluntary regulation VWFA feasible using real-time fMRI neurofeedback. 40 adults typical skills were instructed either upregulate (UP group, N = 20) or downregulate (DOWN their own during six neurofeedback training runs. target was individually defined based on functional localizer task....
Background In countries with German as an official language, children a second language perform overall worse in school than their native speaking peers. This particularly affects written skills, which require advanced knowledge. The reasons are manifold, but one is prominent, namely poor vocabulary Vocabulary, however, consists not only of the number known words also complex and hitherto under‐researched lexico‐semantic framework, referred to depth. Method present study, sample 373 (322...
Aims: Aneurysmal subarachnoid hemorrhage (aSAH) accounts for a large proportion of productive life years lost to stroke. Its management requires multidisciplinary effort. Much the presently available data on incidence, treatment, and outcomes come from studies with strong selection bias, confounding all kinds SAH, that is, aneurysmal nonaneurysmal, cohorts single centers or retrospective series. The aim this study is assess treatment modalities, after SAH national registry. Methods: Patients...
Abstract During the development of reading skills in primary school, children begin to make guesses about unfamiliar words when a text. This process lexical inference is an important source new vocabulary acquisition. In present study, 55 with wide range and knowledge were asked infer meaning unknown (i.e., pseudowords) inserted into short story provide insight their inferencing processes. The results show that use variety cues. While learners higher tend be more successful inferencers rely...
Background: The treatment of sellar lesions demands an interdisciplinary network consisting endocrinologists, neurosurgeons, radio-oncologists, radiosurgeons, ophthalmologists, otorhinolaryngologists, neuropathologists, and neuroradiologists. Each these specialists contributes valuable information to optimize patients; however, a multicenter registry collect the resulting biomedical data was lacking in Switzerland. While other European countries established centers provided base for...
Background: To investigate the pattern of care and outcomes patients with a ruptured intracranial aneurysm who underwent decompressive hemicraniectomy (DH), compare patient characteristics as well short- long-term outcomes. Methods: All data were obtained from Swiss SOS database, at time analysis covering 1,572 treated for aneurysmal subarachnoid hemorrhage (aSAH) one eight neurovascular centers between January 2009 December 2013. Results: From in 197 (12.5%; 66% female, 29% 60 years or...