A5101485213- Acute Myeloid Leukemia Research
- Zebrafish Biomedical Research Applications
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- RNA modifications and cancer
- Congenital heart defects research
- Immune cells in cancer
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Urinary Bladder and Prostate Research
- Mitochondrial Function and Pathology
- Aortic aneurysm repair treatments
- Advanced Sensor and Energy Harvesting Materials
- Prosthetics and Rehabilitation Robotics
- MicroRNA in disease regulation
- Cardiac Ischemia and Reperfusion
- RNA and protein synthesis mechanisms
- Prostate Cancer Diagnosis and Treatment
- Pancreatitis Pathology and Treatment
- Lysosomal Storage Disorders Research
- ATP Synthase and ATPases Research
- Macrophage Migration Inhibitory Factor
- Adenosine and Purinergic Signaling
- Cancer, Hypoxia, and Metabolism
Chinese PLA General Hospital
2023-2025
National Clinical Research Center for Digestive Diseases
2025
Ministry of Education of the People's Republic of China
2025
Nanfang Hospital
2019-2024
Key Laboratory of Guangdong Province
2021-2024
Southern Medical University
2019-2024
Northwest A&F University
2023-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2024
Peking Union Medical College Hospital
2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2021-2024
circRNAs (circular RNAs) are emerging as powerful regulators of cardiac development and disease, but their roles in regeneration still unknown. This study used superenhancers to distinguish key the regulation explored mechanisms underlying circRNA functions.We integrated bioinformatics analysis RNA sequencing data superenhancer catalogs identify superenhancer-associated circRNAs. Quantitative polymerase chain reactions situ hybridization were performed determine expression patterns hearts....
Reactive oxygen species (ROS) derived from oxygen-dependent mitochondrial metabolism are the essential drivers of cardiomyocyte (CM) cell-cycle arrest in adulthood. Mitochondria-localized circular RNAs (circRNAs) play important roles regulating mitochondria-derived ROS production, but their functions cardiac regeneration still unknown. Herein, we investigated and underlying mechanism mitochondria-localized circSamd4 regeneration. We found that was selectively expressed fetal neonatal CMs....
Macrophage polarization plays a crucial role in regulating abdominal aortic aneurysm (AAA) formation. Circular RNAs (circRNAs) are important regulators of macrophage during the development cardiovascular diseases. How-ever, roles circRNAs AAA formation through modulation remain unknown. In present study, we compared circRNA microarray data under two distinct polarizing conditions (M1 and M2 macrophages) identified an M1-enriched circRNA, circCdyl. Loss- gain-of-function assay results...
Metabolic switching during heart development contributes to postnatal cardiomyocyte (CM) cell cycle exit and loss of regenerative capacity in the mammalian heart. control has potential for developing effective CM proliferation strategies. We sought determine whether lactate dehydrogenase A (LDHA) regulated by inducing metabolic reprogramming.LDHA expression was high P1 hearts significantly decreased development. CM-specific LDHA knockout mice were generated using CRISPR/Cas9 technology....
Abdominal aortic aneurysm (AAA) is accepted as a chronic vascular inflammatory disease. However, how the response regulated during AAA formation not fully understood. This study was undertaken to determine whether long noncoding RNA (lncRNA) H19 (H19) promotes by enhancing inflammation. qRT-PCR detected upregulation of in human and mouse tissue samples. Co-staining for macrophage marker MAC-2 showed that located smooth muscle cells (VSMCs) infiltrating macrophages. In vivo overexpression...
Rationale: Most current cardiac regeneration approaches result in very limited cell division and little new cardiomyocyte (CM) mass. Positive feedback loops are vital for division, but their role CM remains unclear. We aimed to determine whether the lncRNA small nucleolar RNA host gene 1 Snhg1 (Snhg1) could form a positive loop with c-Myc induce regeneration. Methods: Quantitative PCR situ hybridization experiments were performed expression patterns fetal myocardial infarction (MI) hearts....
Mature broccoli has large flower balls and thick stems. Therefore, manual picking is laborious energy-consuming. However, the big spheroid vegetable-picking manipulator a complex structure poor enveloping effect easily causes mechanical damage. ball-picking with compact simple control system was designed. The smart in stable configuration when enveloped balls. First, physical damage test carried out on according to underactuated manipulator’s design scheme. maximum surface pressure of ball...
Abstract In adverse environments, the number of fertilizable female gametophytes (FGs) in plants is reduced, leading to increased survival remaining offspring. How maternal plant perceives internal growth cues and external stress conditions alter FG development remains largely unknown. We report that homeostasis signaling molecule nitric oxide (NO) plays a key role controlling under both optimal conditions. NO precisely regulated by S-nitrosoglutathione reductase (GSNOR). Prior...
Haploinsufficiency of the ATP6V1B2, a subunit V-ATPases, underlies genetic disorders including Dominant deafness-onychodystrophy (DDOD), deafness, onychodystrophy, osteodystrophy, mental retardation and seizures (DOORS), Zimmermann-Laband syndromes, all characterized by congenital hearing loss onychodystrophy. Effective therapies for ATP6V1B2-associated remain elusive. The study generates hair cell-specific knockout mouse (Atp6v1b2fl/fl;Atoh1Cre/+) recapitulating human phenotypes, with...
Abstract Post-translational modification (PTM) by small ubiquitin-like modifier (SUMO) is a key regulator of cell proliferation and can be readily reversed family SUMO-specific proteases (SENPs), making SUMOylation an ideal regulatory mechanism for developing novel therapeutic strategies promoting cardiac regenerative response. However, the role in regeneration remains unknown. In present study, we assessed whether targeting protein kinase B (Akt) promote regeneration. Quantitative PCR...
Mesenchymal stem/stromal cells (MSCs) are of great therapeutic value due to their role in maintaining the function hematopoietic stem/progenitor (HSPCs). MSCs derived from human pluripotent stem represent an ideal alternative because unlimited supply. However, with neural crest origin HPSCs on maintenance HSPCs has not been reported. Flow cytometric analysis, RNA sequencing and differentiation ability were applied detect characteristics stromal 3D brain organoids. Human umbilical cord blood...
Abstract Stimulating cardiomyocyte (CM) dedifferentiation and cell cycle activity (DACCA) is essential for triggering daughter CM formation. In addition to transcriptional processes, RNA‐binding proteins (RBPs) are emerging as crucial post‐transcriptional players in regulating DACCA. However, whether regulation of DACCA by RBPs could effectively trigger formation remains unknown. By performing integrated bioinformatic analysis snRNA‐seq data from neonatal adult hearts, this study identified...
Abstract Antagonism of ROS signaling can inhibit cell apoptosis and autophagy, thus favoring the maintenance expansion hematopoietic stem cells. Alpha lipoic acid (ALA), a small antioxidant molecule, affects by lowering level. In this study, we show that ALA promoted production human pluripotent cells (hPSCs) derived hemogenic endothelial stem/progenitor in vitro. Transcriptome analysis hPSCs showed endothelial-to-hematopoietic transition up-regulating RUNX1, GFI1, GFI1B, MEIS2, HIF1A...
Abstract Mast cells (MCs) play a pivotal role in the hypersensitivity reaction by regulating innate and adaptive immune responses. Humans have two types of MCs. The first type, termed MCTC, is found skin other connective tissues expresses both tryptase chymase, while second, MCT, which only tryptase, primarily mucosa. MCs induced from human adult-type CD34+ are reported to be MCT but development during embryonic/fetal stages largely unknown. Using an efficient coculture system, we identified...
As a novel bionic actuator, pneumatic artificial muscle has high power to weight ratio. In this paper, variable camber wing with the is developed. Firstly, experimental setup measure static output force of designed. The relationship between and air pressure investigated. Experimental result shows decreases nonlinearly increasing contraction Secondly, finite element model Numerical results show that tip displacement trailing-edge increases linearly external load limited maximum muscles....
As a novel bionic actuator, pneumatic artificial muscle has high power to weight ratio. In this paper, the experimental setup measure static output force of was designed and relationship between air pressure investigated. Experimental result shows decreases nonlinearly with increasing contraction A variable camber wing based on developed model manufactured validate concept. Wind tunnel tests were conducted in low speed wind tunnel. that increases pressure.
p15INK4b (cyclin-dependent kinase inhibitor 2B, CDKN2B, p15), a cyclin-dependent (CKI) belonging to the INK4 family, plays an important role in hematopoiesis. Its expression level was positively related blockage effects of RUNX1b at early stage. Experiments using human embryonic stem cell (hESC) lines with inducible p15 suggested that overexpression can significantly decrease proportion KDR+ cells S and G2-M stages 4 days after induction from day 0. Moreover, stage production CD34highCD43-...
Erythroid and myeloid differentiation disorders are commonly occurred in leukemia. Given that the relationship between erythroid lineages is still unclear. To find co-regulators might help to new target for therapy of In hematopoiesis, ALA (alpha lipoic acid) reported inhibit neutrophil lineage determination by targeting transcription factor ELK1 granulocyte-monocyte progenitors via splicing SF3B1. However, further exploration needed determine whether a common regulatory differentiation....
ABSTRACT Aim To investigate whether oral microbiome diversity is associated with all‐cause mortality in the general US population and individuals chronic diseases. Materials Methods We included 8224 data from National Health Nutrition Examination Survey (2009–2012), representing 164,000,205 adults, using a survey‐weighted analysis method. Cox regression analyses were performed to identify association between mortality. Results During mean follow‐up period of 8.86 years, 429 deaths...