A5062610521- Arsenic contamination and mitigation
- Chromium effects and bioremediation
- Metal Extraction and Bioleaching
- Genomics and Phylogenetic Studies
- Bacterial biofilms and quorum sensing
- Mine drainage and remediation techniques
- Environmental Toxicology and Ecotoxicology
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- Biocrusts and Microbial Ecology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Heavy metals in environment
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Transgenic Plants and Applications
- Mycorrhizal Fungi and Plant Interactions
- Aquaculture disease management and microbiota
- Genomics and Chromatin Dynamics
- Enzyme-mediated dye degradation
- RNA regulation and disease
Centre National de la Recherche Scientifique
2015-2024
Université de Strasbourg
2015-2024
Institut de Biologie Moléculaire des Plantes
2016-2024
Génétique Moléculaire Génomique Microbiologie
2006-2020
Microbial biotransformations have a major impact on contamination by toxic elements, which threatens public health in developing and industrial countries. Finding means of preserving natural environments-including ground surface waters-from arsenic constitutes challenge facing modern society. Although this metalloid is ubiquitous Earth, thus far no bacterium thriving arsenic-contaminated environments has been fully characterized. In-depth exploration the genome beta-proteobacterium...
Bacteria of the Thiomonas genus are ubiquitous in extreme environments, such as arsenic-rich acid mine drainage (AMD). The genome one these strains, sp. 3As, was sequenced, annotated, and examined, revealing specific adaptations allowing this bacterium to survive grow its highly toxic environment. In order explore genomic diversity well genetic evolution spp., a comparative hybridization (CGH) approach used on eight different strains genus, including five same species. Our results suggest...
Abstract Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in network connecting several translational repressors/decapping activators. directly interacts with DECAPPING 5 (DCP5), ortholog of human LSM14 and yeast Scd6, this interaction connects to additional decay factors like DDX6/Dhh1-like RNA helicases....
Both the speciation and toxicity of arsenic are affected by bacterial transformations, i.e. oxidation, reduction or methylation. These transformations have a major impact on environmental contamination more particularly drinking water. Herminiimonas arsenicoxydans has been isolated from an arsenic- contaminated environment developed various mechanisms for coping with arsenic, including oxidation As(III) to As(V) as detoxification mechanism.In present study, differential transcriptome...
Abstract Background Arsenic is present in numerous ecosystems and microorganisms have developed various mechanisms to live such hostile environments. Herminiimonas arsenicoxydans , a bacterium isolated from arsenic contaminated sludge, has acquired remarkable capabilities cope with arsenic. In particular our previous studies suggested the existence of temporal induction arsenite oxidase, key enzyme metabolism, presence As(III). Results Microarrays were designed compare gene transcription...
Arsenic is widespread in the environment and its presence a result of natural or anthropogenic activities. Microbes have developed different mechanisms to deal with toxic compounds such as arsenic this resist metabolize compound. Here, we present first reference set genomic, transcriptomic proteomic data an Alphaproteobacterium isolated from arsenic-containing goldmine: Rhizobium sp. NT-26. Although phylogenetically related plant-associated bacteria, organism has lost major colonizing...
An arsenite-oxidizing bacterium, designated strain ULPAs1(T), was isolated from industrial sludge heavily contaminated with arsenic. Cells of this isolate were Gram-negative, curved rods, motile by means a polar flagellum. The positive for oxidase and catalase activities, able to reduce nitrate nitrite, used acetate, lactate peptone as organic carbon sources under aerobic conditions oxidize arsenite (As[III]) arsenate (As[V]). 16S rRNA gene sequence analysis the absence dodecanoic fatty...
Biofilms represent the most common microbial lifestyle, allowing survival of populations exposed to harsh environmental conditions. Here, we show that biofilm development a bacterial species belonging Thiomonas genus, frequently found in arsenic polluted sites and playing key role natural remediation, is markedly modified when subinhibitory doses this toxic element. Indeed, arsenite [As(III)] exposure led considerable impact on maturation by strongly increasing extracellular matrix synthesis...
Herminiimonas arsenicoxydans is a Gram-negative bacterium able to detoxify arsenic-contaminated environments by oxidizing arsenite [As(III)] arsenate [As(V)] and scavenging arsenic ions in an extracellular matrix. Its motility colonization behaviour have been previously suggested be influenced arsenite. Using time-course confocal laser scanning microscopy, we investigated its biofilm development the absence presence of Arsenite was shown delay initiation wild-type strain; this partly...
Abstract Microorganisms dwelling in sediments have a crucial role biogeochemical cycles and are expected to strong influence on the cycle of arsenic, metalloid responsible for severe water pollution presenting major health risks human populations. We present here metagenomic study sediment from two harbours M editerranean F rench coast, l' E staque S t andrier. The first site is highly polluted with arsenic heavy metals, while concentration second below toxicity levels. goal this was...
Rhodococcus strain IGTS8 is the most extensively studied model bacterium for biodesulfurization of fossil fuels via non–destructive sulfur–specific 4S pathway. However, taxonomic status this strain, which was initially assigned to species rhodochrous and later erythropolis, controversial. Phylogenomic analyses whole genome closely related rhodococci showed that R. erythropolis qingshengii are very species, a several strains identified as should be re-classified qingshengii. The genomes each...
Acid mine drainage (AMD) is a highly toxic environment for most living organisms due to the presence of many lethal elements including arsenic (As). Thiomonas (Tm.) bacteria are found ubiquitously in AMD and can withstand these extreme conditions, part because they able oxidize arsenite. In order further improve our knowledge concerning adaptive capacities bacteria, we sequenced assembled genome six isolates derived from Carnoulès AMD, compared them genomes Tm. arsenitoxydans 3As (isolated...
Arsenic is a toxic metalloid known to generate an important oxidative stress in cells. In the present study, we focused our attention on alga related genus Coccomyxa, exhibiting extraordinary capacity resist high concentrations of arsenite and arsenate. The integrated analysis high-throughput transcriptomic data non-targeted metabolomic approaches highlighted multiple levels protection against arsenite. Indeed, Coccomyxa sp. Carn induced set transporters potentially preventing accumulation...
Abstract Viral RNAs can be uridylated in eukaryotic hosts. However, our knowledge of uridylation patterns and roles remains rudimentary for phytoviruses. Here, we report global 3′ terminal RNA profiles representatives the main families positive single-stranded We detected all 47 viral investigated here, revealing its prevalence. Yet, levels varied from 0.2% to 90%. Unexpectedly, most poly(A) tails grapevine fanleaf virus (GFLV) RNAs, including encapsidated tails, were strictly...
Nicotiana otophora contains Agrobacterium-derived T-DNA sequences introduced by horizontal gene transfer (Chen et al., 2014). Sixty-nine contigs were assembled into four different cellular T-DNAs (cT-DNAs) totalling 83 kb. TC and TE result from two successive transformation events, each followed duplication, yielding inserts. is also found in other species, whereas unique to N. otophora. Both cT-DNA regions are partially duplicated inverted repeats. Analysis of the divergence patterns...
The acid mine drainage (AMD) in Carnoulès (France) is characterized by the presence of toxic metals such as arsenic. Several bacterial strains belonging to Thiomonas genus, which were isolated from this AMD, are able withstand these conditions. Their genomes carry several genomic islands (GEIs), known be potentially advantageous some particular ecological niches. This study focused on role "urea island" present CB2 strain, genes involved urea degradation processes. First, comparisons showed...
The involvement of the different Lactuca species in domestication and diversification cultivated lettuce is not totally understood. serriola considered as direct ancestor closest relative to sativa , while other wild that can be crossed with L. virosa saligna would have just contributed latter typologies. To contribute study evolution, we assembled mtDNA genomes nine spp. accessions, among them three from whose had been studied so far. Our results unveiled little no intraspecies variation...
Over the course of evolution, land plant mitochondrial genomes have lost many transfer RNA (tRNA) genes and import nucleus-encoded tRNAs is essential for protein synthesis. By contrast, plastidial photosynthetic plants generally possess a complete set tRNA existence remains long-standing question. The early vascular Selaginella genus show an extensive loss while retaining capacity, represent ideal model answering this Using purification, northern blot hybridization, high-throughput...
ABSTRACT We report the genome sequence of Halomonas sp. strain A3H3, a bacterium with high tolerance to arsenite, isolated from multicontaminated sediments l'Estaque harbor in Marseille, France. The is composed 5,489,893-bp chromosome and 157,085-bp plasmid.
Thymidine kinase (TK) is a key enzyme of the salvage pathway that recycles thymidine nucleosides to produce deoxythymidine triphosphate. Here, we identified single TK maize (Zea mays), denoted CPTK1, as necessary in replication plastidial genome (cpDNA), demonstrating essential function during chloroplast biogenesis. CPTK1 localized both plastids and mitochondria, its absence resulted an albino phenotype, reduced cpDNA copy number severe deficiency ribosomes. Mitochondria were not affected,...