A5112480966- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Sperm and Testicular Function
- Histone Deacetylase Inhibitors Research
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- Wnt/β-catenin signaling in development and cancer
- RNA and protein synthesis mechanisms
- Genetic Neurodegenerative Diseases
- Nuclear Receptors and Signaling
- Air Quality and Health Impacts
- Protein Degradation and Inhibitors
- Acute Myeloid Leukemia Research
- Neurological disorders and treatments
- Cancer Cells and Metastasis
- Per- and polyfluoroalkyl substances research
- Reproductive Biology and Fertility
- Clinical Laboratory Practices and Quality Control
- Multiple Myeloma Research and Treatments
- RNA Research and Splicing
- Biofuel production and bioconversion
- Bacterial biofilms and quorum sensing
- Lung Cancer Research Studies
- Trace Elements in Health
Yunnan University
2024-2025
Shanghai Pudong New Area Gongli Hospital
2025
Second Military Medical University
2025
Sichuan Normal University
2025
Chongqing University
2023-2024
Nantong University
2022-2024
Shaanxi Normal University
2021-2024
First Hospital of China Medical University
2024
Fujian Medical University
2023-2024
Hunan Agricultural University
2021-2024
Circulating tumor DNA (ctDNA) holds great promise as a noninvasive biomarker for cancer diagnosis, treatment, and prognosis. However, the accurate specific quantification of low-abundance ctDNA in serum remains significant challenge. This study introduced, first time, novel exponential amplification reaction (EXPAR)-assisted CRISPR/Cas12a-mediated ratiometric dual-signal electrochemical biosensor ultrasensitive reliable detection ctDNA. To implement strategy, signal unit (ssDNA-MB@Fc/UiO-66-NH
Acute colitis is a complex disease that can lead to dysregulation of the gut flora, inducing more parenteral diseases. Dandelion polysaccharides (DPSs) may have potential preventive and therapeutic effects on enteritis. In this study, LPS was used induce enteritis VC as positive drug control explore DPS The results showed could repair intestinal barrier, down-regulate expression TNF-α, IL-6, IL-1β, other pro-inflammatory factors, up-regulate IL-22 anti-inflammatory factor, improve...
Accurate detection of cancer-associated mRNAs is beneficial to early diagnosis and potential treatment cancer. Herein, for the first time, we developed a novel CRISPR/Cas12a-powered electrochemical/fluorescent (EC/FL) dual-mode controlled-release homogeneous biosensor mRNA detection. A functionalized ssDNA P2-capped Fe3O4–NH2 loaded with methylene blue (P2@MB–Fe3O4–NH2) was synthesized as signal probe, while survivin chosen target RNA. In presence mRNA, nicking endonuclease-mediated rolling...
Abstract Environmental toxicants, such as cadmium, found in foods, water, and consumer products are known to induce male reproductive dysfunction. However, the underlying molecular mechanism(s) by which cadmium-induced Sertoli cell injury manifested a disruption of blood-testis barrier (BTB) remains unknown. Interestingly, one primary targets cadmium toxicity testis is cytoskeletons cells, which, turn, impedes junctions seminiferous epithelium. In order expand these earlier observations...
We evaluated the drying characteristics and structure, as well physicochemical flavor properties, of G. elata treated by hot-air (HAD), vacuum (VD), freeze (FD), microwave (MD), (MVD). found that MD MVD showed shortest times, while FD were able to better retain active ingredients color samples. However, different methods did not change internal structure elata, its main components fundamentally change. In addition, E-nose HS-SPME-GC-MS effectively differentiated volatile components, 36...
Levilactobacillus brevis YT108, identified for its ability to metabolize prebiotic xylo-oligosaccharides (XOS), emerges as a candidate probiotic use in synbiotic food formulations. This study aimed investigate the metabolic and genomic traits associated with XOS metabolism YT108 assess attributes through whole genome sequencing vitro assays. Strain exhibited robust growth kinetics on sole carbon source, profile comparable that glucose, achieving pH reduction 4.68 an OD600 nm of 1.603 after...
The development of multidrug resistance (MDR) during postoperative chemotherapy for colorectal cancer substantially reduces therapeutic efficacy. Nanostructured drug delivery systems (NDDSs) with modifiable chemical properties are considered promising candidates as therapies reversing MDR in cells. Selenium-doped manganese phosphate (Se-MnP) nanoparticles (NPs) that can reverse through sustained release selenium have the potential to improve effect cancer.Se-MnP NPs had an organic-inorganic...
This study was conducted to examine the influences of replacing soybean meal (SBM) with fermented (FSBM) in diet lactating Holstein cattle on rumen fermentation and ruminal bacterial microbiome. Twenty-four Chinese dairy were assigned each two treatments a completely randomized design: SBM group [the basal total mixed ration (TMR) containing 5.77% SBM] FSBM (the experimental TMR 5.55% FSBM). trial lasted for 54 days (14 adjustment 40 data sample collection), samples liquid collected 34 d d,...
Despite the known reproductive toxicity induced by triptolide (TP) exposure, regulatory mechanism underlying testicular vacuolization injury caused TP remains largely obscure.
Per- and polyfluoroalkyl substances (PFASs) are a family of "forever chemicals" including perfluorooctane sulfonate (PFOS). These toxic chemicals do not break down in the environment or our bodies. In human body, PFOS perfluoroctanoic acid (PFOA) have half-life (
Herein, a universal nucleic acid analysis platform was constructed to sensitively and accurately detect miRNA-155 ctDNA using isothermal amplification-assisted CRISPR/Cas12a DNA tetrahedral nanostructures (TDN) supported sensing interface.
Sysmex DI-60 enumerates and classifies leukocytes. Limited research has evaluated the performance of in abnormal samples, most focused on leukopenic samples. We evaluate efficacy determining white blood cell (WBC) differentials normal samples different WBC count. Peripheral smears (n = 166) were categorised into control disease groups, further divided moderate severe leucocytosis, mild normal, leukopenia, leukopenia groups based preclassification verification manual counting results assessed...
Multiple myeloma (MM) is a hematological malignancy that characterized by the clonal expansion of plasma cells in bone marrow. Histone deacetylases (HDACs) represent new type molecular targeted therapy for different types cancers and promising targets therapy. We showed HDAC3 mRNA protein levels CD138 mononuclear from MM patients were higher than those healthy donors. Therefore, we investigated effects novel class I HDAC inhibitor BG45 on vitro. downmodulated heme oxygenase 1 (HO-1) when...
Lymphoid enhancer-binding factor 1 (LEF1) is a key transcription mediating the Wnt signaling pathway. LEF1 regulator that closely associated with tumor malignancy and usually upregulated in cancers, including colonic adenocarcinoma. The underlying molecular mechanisms of regulation for adenocarcinoma progression remain unknown. To explore it, expression caco2 cells was inhibited using an shRNA approach. results showed downregulation motility microstructures, such as polymerization F-actin,...