A5042815762- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Inflammatory mediators and NSAID effects
- Spine and Intervertebral Disc Pathology
- Natural product bioactivities and synthesis
- Adipokines, Inflammation, and Metabolic Diseases
- Psoriasis: Treatment and Pathogenesis
- Amyotrophic Lateral Sclerosis Research
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Orthopaedic implants and arthroplasty
- Fault Detection and Control Systems
- Spondyloarthritis Studies and Treatments
- Fluid Dynamics and Mixing
- Musculoskeletal pain and rehabilitation
- Circular RNAs in diseases
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Orthopedic Infections and Treatments
- Dermatology and Skin Diseases
- Total Knee Arthroplasty Outcomes
- Advanced biosensing and bioanalysis techniques
- Coenzyme Q10 studies and effects
- IL-33, ST2, and ILC Pathways
Qilu Hospital of Shandong University
2014-2025
Affiliated Eye Hospital of Wenzhou Medical College
2024
Wenzhou Medical University
2024
Second Hospital of Shandong University
2019-2024
China University of Petroleum, Beijing
2023-2024
Fudan University
2020-2023
Shandong University
2018-2022
New York University
2013-2016
Columbia University Irving Medical Center
2013-2016
New York University Langone Orthopedic Hospital
2012-2014
Excessive mechanical stress is closely associated with cell death in various conditions. Exposure of chondrocytes to excessive loading leads a catabolic response as well exaggerated death. Ferroptosis recently identified form during aging and degeneration. However, it's potential association remains be illustrated.To identify whether can cause ferroptosis. To explore the role overloading chondrocyte ferroptosis.Chondrocytes were collected from unloading zones cartilage patients...
Objective Progranulin (PGRN) was previously isolated as an osteoarthritis (OA)-associated growth factor. Additionally, PGRN found to play a therapeutic role in inflammatory arthritis mice models through antagonising tumour necrosis factor α (TNF-α). This study aimed at investigating the of degradation cartilage and progression OA. Methods Progression OA analysed both spontaneous surgically induced wild type PGRN-deficient mice. Cartilage were evaluated using Safranin O staining,...
Background: Intervertebral disc (IVD) degeneration is a common degenerative disease that can lead to collapse or herniation of the nucleus pulposus (NP) and result in radiculopathy patients. Methods: NP tissue cells were isolated from patients mice, expression profile cortistatin (CST) was analysed. In addition, ageing compared between 6-month-old WT CST-knockout (CST-/-) mice. Furthermore, tissues cultured validate role CST TNF-α-induced IVD degeneration. Moreover, vitro vivo experiments...
Gaucher disease (GD) is a genetic caused by mutations in the GBA1 gene which result reduced enzymatic activity of β-glucocerebrosidase (GCase). This study identified progranulin (PGRN) (GRN) as another associated with GD.Serum levels PGRN were measured from 115 GD patients and 99 healthy controls, whole GRN 40 was sequenced, genotyping 4 SNPs performed 161 142 control samples. Development PGRN-deficient mice characterized, therapeutic effect rPGRN on analyzed.Serum significantly lower...
Microneedle patches have become an exciting means for transdermal delivery of various therapeutics. Herein, we report on self-sterilizing dissolving nanosilver-loaded microneedle created from carboxymethylcellulose capable suppressing microbial pathogen growth at the insertion site.
Osteoarthritis (OA) is a common degenerative disease, and tumor necrosis factor (TNF-α) known to play critical role in OA. Cortistatin (CST) neuropeptide discovered over 20 years ago, which plays vital inflammatory reactions. However, it unknown whether CST involved cartilage degeneration OA development.The interaction between TNF-α receptors was investigated through Coimmunoprecipitation Biotin-based solid-phase binding assay. Western blot, Real-time PCR, ELISA, immunofluorescence staining,...
<h3>Objective</h3> To examine the expression of ADAMTS-7 during progression osteoarthritis (OA), defining its role in pathogenesis OA, and elucidating molecular events involved. <h3>Methods</h3> cartilage a rat OA model was assayed using immunohistochemistry. Cartilage-specific transgenic mice small interfering (si)RNA knockdown were generated used to analyse both spontaneous surgically induced models. Cartilage degradation evaluated Safranin-O staining, immunohistochemistry, ELISA western...
Osteoarthritis (OA) is a common chronic degenerative disease characterized by degeneration in the joints and subsequent destruction of cartilage bone, yet much remains to be elucidated regarding its molecular mechanism. Ghrelin recently discovered neuropeptide with anti-inflammatory actions, but it unknown whether ghrelin involved OA. Human primary chondrocyte samples were collected from patients OA, expression pattern was assessed. stimulated IL-1β TNF-α, exogenous ghrelin-alleviated...
Aseptic loosening is a major complication of prosthetic joint surgery, characterized by chronic inflammation, pain, and osteolysis surrounding the bone-implant interface. Progranulin (PGRN) known to have anti-inflammatory action binding Tumor Necrosis Factor (TNF) receptors antagonizing TNFα. Here we report that titanium particles significantly induced PGRN expression in RAW264.7 cells also mouse air-pouch model inflammation. PGRN-deficiency enhanced, whereas administration recombinant...
Abstract Contact dermatitis and psoriasis are skin disorders caused by immune dysregulation, yet much remains unknown about their underlying mechanisms. Ghrelin, a recently discovered novel peptide potential endogenous anti-inflammatory factor expressed in the epidermis, is involved repair disease. In this study, we investigated expression pattern therapeutic effect of ghrelin both contact mouse models induced oxazolone (OXA) imiquimod (IMQ), respectively, TNF-α-stimulated RAW264.7...
Abstract Intervertebral disc (IVD) degeneration is a common degenerative disease, yet much unknown about the mechanisms during its pathogenesis. Herein we investigated whether progranulin (PGRN), chondroprotective growth factor, associated with IVD degeneration. PGRN was detectable in both human and murine IVD. The levels of were upregulated tissue aging process. Loss resulted an early onset changes altered expressions degeneration-associated molecules mouse tissue. Moreover, knockout mice...
Near work is generally considered as a risk factor for myopia onset and progression. This study aimed to investigate the choroidal responses brief-period of near in children young adults. Thirty myopic medical students (aged 18-28 years) 30 8-12 participated this study. The submacular total area (TCA), luminal (LA), stromal (SA), vascularity index (CVI) choriocapillaris flow deficit (CcFD), well subfoveal thickness (SFCT) were measured with swept-source optical coherence tomography/optical...
Abstract Implant‐associated infections and inflammation during pre‐ post‐operative procedures remain significant challenges that reduce implant longevity. In this study, the uniques bioactive coatings derived from silver‐gallium liquid metal particles (Ag‐GaNPs) deposited uniformly over HAp scaffold (HAp‐Ag‐GaNPs) are developed. The HAp‐Ag‐GaNPs exhibit strong antimicrobial activities against Gram‐negative Pseudomonas aeruginosa , Gram‐positive Staphylococcus aureus ( S. ), drug‐resistant...
Developing rapid and sensitive photoelectrochemical (PEC) platforms for detecting alpha-fetoprotein (AFP) is of utmost importance. A PEC aptasensor system was constructed in this investigation to specifically detect AFP. The visible...
Destabilization of medial meniscus (DMM) model is an important tool for studying the pathophysiological roles numerous arthritis associated molecules in pathogenesis osteoarthritis (OA) vivo. However, detailed, especially visualized protocol establishing this complicated mice, not available. Herein we took advantage wildtype and progranulin (PGRN)-/- mice as examples to introduce a inducing DMM compared onset OA following establishment surgically induced model. The operations performed on...
Follistatin-like protein 1 (FSTL1) is a well-known mediator of inflammation. Intervertebral disc disease an inflammatory disorder. Here, we investigated the role FSTL1 in intervertebral discs inflammation.Expression nucleus pulposus tissues from rats and human was determined by immunohistochemistry staining western blot analysis. The expression levels tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β) matrix metalloproteinase 13 (MMP-13) rat were measured staining. mitogen-activated...
Growth differentiation factor 11 (GDF11), a key member of the TGF-β superfamily, plays critical roles in various medical conditions. Recently, GDF11 was found to suppress nuclear kappa-light-chain-enhancer activated B cells (NF-κB) signaling pathway and protect against inflammation. This study aimed investigate role development rheumatoid arthritis (RA). We demonstrated that treatment antagonized TNF-α-induced inflammation macrophages. Moreover, inhibited collagen-induced collagen...
// Hong Ding 1, * , Jianlu Wei Yunpeng Zhao 1 Yi Liu Lian and Lei Cheng Department of Orthopaedics, Qilu Hospital Shandong University, Jinan, China These authors have contributed equally to this work Correspondence to: Cheng, email: chengleiyx@126.com Keywords: Atsttrin; TNF-α; progranulin; intervertebral disc degeneration; inflammation Received: September 06, 2017 Accepted: October 29, Published: November 2017 ABSTRACT Atsttrin, an engineered molecule...