A5005268235- Bone Metabolism and Diseases
- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- S100 Proteins and Annexins
- Amyotrophic Lateral Sclerosis Research
- Bone health and treatments
- Spinal Fractures and Fixation Techniques
- Inflammatory mediators and NSAID effects
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Musculoskeletal pain and rehabilitation
- Cervical and Thoracic Myelopathy
- Metabolism, Diabetes, and Cancer
- Connective Tissue Growth Factor Research
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Spondyloarthritis Studies and Treatments
- Medical Imaging and Analysis
- Protease and Inhibitor Mechanisms
- Orthopaedic implants and arthroplasty
- Antimicrobial Peptides and Activities
- Bone and Joint Diseases
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Pregnancy-related medical research
Qilu Hospital of Shandong University
2013-2025
Changsha University of Science and Technology
2024-2025
New York University
2014-2024
Shandong University
2013-2023
Third Hospital of Hebei Medical University
2021-2022
Hebei Medical University
2021-2022
First Affiliated Hospital of Hebei Medical University
2022
Shandong First Medical University
2016-2022
Columbia University Irving Medical Center
2014-2021
ORCID
2021
Rho-associated kinase 2 (ROCK2) regulates the secretion of proinflammatory cytokines and development autoimmunity in mice. Data from a phase 1 clinical trial demonstrate that oral administration KD025, selective ROCK2 inhibitor, to healthy human subjects down-regulates ability T cells secrete IL-21 IL-17 by 90% 60%, respectively, but not IFN-γ response T-cell receptor stimulation vitro. Pharmacological inhibition with KD025 or siRNA-mediated ROCK2, ROCK1, significantly diminished STAT3...
Objective Progranulin (PGRN) was previously isolated as an osteoarthritis (OA)-associated growth factor. Additionally, PGRN found to play a therapeutic role in inflammatory arthritis mice models through antagonising tumour necrosis factor α (TNF-α). This study aimed at investigating the of degradation cartilage and progression OA. Methods Progression OA analysed both spontaneous surgically induced wild type PGRN-deficient mice. Cartilage were evaluated using Safranin O staining,...
Human umbilical cord mesenchymal stem cells (huMSCs) can treat primary ovarian insufficiency (POI) related to granulosa cell (OGC) apoptosis caused by cisplatin chemotherapy. Exosomes are a class of membranous vesicles with diameters 30-200 nm that constitutively released eukaryotic cells. mediate local cell-to-cell communication transferring microRNAs and proteins. In the present study, we demonstrated effects exosomes derived from huMSCs (huMSC-EXOs) on cisplatin-induced OGC model in vitro...
Gaucher disease (GD), the most common lysosomal storage disease, is caused by mutations in GBA1 encoding of β-glucocerebrosidase (GCase). Recently it was reported that progranulin (PGRN) insufficiency and deficiency associated with GD human mice, respectively. However underlying mechanisms remain unknown. Here we report PGRN binds directly to GCase its results aggregation receptor LIMP2. Mass spectrometry approaches identified HSP70 as a GCase/LIMP2 complex-associated protein upon stress, an...
Atsttrin, an engineered protein composed of three tumor necrosis factor receptor (TNFR)-binding fragments progranulin (PGRN), shows therapeutic effect in multiple murine models inflammatory arthritis . Additionally, intra-articular delivery PGRN protects against osteoarthritis (OA) progression. The purpose this study is to determine whether Atsttrin also has effects OA and the molecular mechanisms involved.Surgically induced noninvasive rupture were established mouse rat, respectively....
Aseptic loosening is a major complication of prosthetic joint surgery, characterized by chronic inflammation, pain, and osteolysis surrounding the bone-implant interface. Progranulin (PGRN) known to have anti-inflammatory action binding Tumor Necrosis Factor (TNF) receptors antagonizing TNFα. Here we report that titanium particles significantly induced PGRN expression in RAW264.7 cells also mouse air-pouch model inflammation. PGRN-deficiency enhanced, whereas administration recombinant...
Spinal cord injury (SCI) is a complex problem in modern medicine. Fibroblast activation and fibroscarring after SCI impede nerve recovery. Non-coding RNA plays an important role the progression of many diseases, but study its spinal fibrosis still emerging. Here, we investigated function circular RNAs, specifically antisense to cerebellar degeneration-related protein 1 (CDR1as), characterized molecular mechanism pathophysiology. The presence CDR1as was verified by sequencing expression...
The electroreductive conversion of waste nitrate (NO₃⁻) to high-value ammonia (NH₃) offers an alternative the energy-intensive Haber-Bosch process. However, this reaction involves multistep electron-coupled proton transfer, posing kinetic challenges for NH₃ generation. Herein, a-CuCoOx-based tandem electrocatalyst nitrate-to-ammonia is presented. In 1 M KOH with 50 mM NO₃⁻, amorphous catalyst achieves a Faradaic efficiency (FE) 95.61% and yield rate 4.01 mg h⁻¹ cm⁻2 at -0.3 V versus RHE,...
We previously reported that marchantin M (Mar) is an active agent to induce apoptosis in human prostate cancer (PCa), but the molecular mechanisms of action remain largely unknown. Here, we demonstrate Mar potently inhibited chymotrypsin-like and peptidyl-glutamyl peptide-hydrolyzing activities 20S proteasome both vitro intracellular systems significantly induced accumulation polyubiquitinated proteins PCa cells. The computational modeling analysis suggested non-covalently bound sites β5 β1...
Follistatin-like protein 1 (FSTL1) is a well-known mediator of inflammation. Intervertebral disc disease an inflammatory disorder. Here, we investigated the role FSTL1 in intervertebral discs inflammation.Expression nucleus pulposus tissues from rats and human was determined by immunohistochemistry staining western blot analysis. The expression levels tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β) matrix metalloproteinase 13 (MMP-13) rat were measured staining. mitogen-activated...
// Hong Ding 1, * , Jianlu Wei Yunpeng Zhao 1 Yi Liu Lian and Lei Cheng Department of Orthopaedics, Qilu Hospital Shandong University, Jinan, China These authors have contributed equally to this work Correspondence to: Cheng, email: chengleiyx@126.com Keywords: Atsttrin; TNF-α; progranulin; intervertebral disc degeneration; inflammation Received: September 06, 2017 Accepted: October 29, Published: November 2017 ABSTRACT Atsttrin, an engineered molecule...
Diabetes mellitus is a group of chronic diseases characterized by high blood glucose levels. Diabetic patients have higher risk sustaining osteoporotic fractures than non-diabetic people. The fracture healing usually impaired in diabetics, and our understanding the detrimental effects hyperglycemia on still inadequate. Metformin first-line medicine for type 2 diabetes (T2D). However, its bone T2D remain to be studied. To assess impacts metformin healing, we compared process closed-wound...
Background Intervertebral disc degeneration (IVDD) is a leading cause of low back pain (LBP). The pathological process IVDD associated with inflammatory reactions and extracellular matrix (ECM) disorders. Digoxin widely used for treating heart failure, it has been reported to have anti-inflammatory effects. Objective This study investigate the role digoxin in pathogenesis intervertebral as well involved molecular mechanism, particularly potential target protein. Methods We exploited rat...
Abstract Type 2 diabetes mellitus (T2DM) significantly increases bone fragility and fracture risk. Progranulin (PGRN) promotes healing in both physiological type 1 diabetic conditions. The present study aimed to investigate the role of PGRN T2DM healing. MKR mice (with an FVB/N genetic background) were used as model. Drill‐hole Bonnarens Einhorn models vivo . Primary marrow cells isolated for molecular signaling studies, reverse transcription‐polymerase chain reaction, immunohistochemical...
Objective: Fexofenadine (FFD) is an antihistamine drug with anti-inflammatory effect. The intervertebral disc (IVD) degeneration process involved in inflammation which tumor necrosis factor-α (TNF-α) plays important role. This study aims to investigate the role of FFD pathological IVD degeneration. Methods: Safranin O staining was used for measurement cartilageous tissue disc. Hematoxylin-Eosin (H&E) determine construction. A rat needle puncture model taken advantage examine vivo ....
The spinal cord rarely repairs itself when damaged; however, methods for encouraging nerves to regrow are on the horizon. Although circular RNAs (circRNAs) contribute various biological processes, including neuronal their functions in subacute phase of injury (SCI) have not been elucidated. In this study, we identified a novel circRNA, named CircPlek, with increased expression tissues after SCI.We predicted regulatory relationship between CircPlek and miR-135b-5p, which showed most obvious...
Indirubin, a traditional Chinese medicine, is used to treat autoimmune diseases in clinics. However, the effects of indirubin on immunosuppressive CD4+CD25+Foxp3+ regulatory T cells (Treg) have not been addressed. Thus, we aimed investigate CD4+CD25+Treg immune thrombocytopenia (ITP) CBA mice, which were established by immunization with Wistar rat platelets. 50 mg/kg treatment daily for 4 weeks significantly decreased anti-platelet antibody production and prevented decrease platelets caused...
Inflammation plays an important role in intervertebral disc degeneration (IDD). The protein follistatin-like 1 (FSTL1) a proinflammatory variety of inflammatory diseases.The purpose this study was to investigate whether IDD could be delayed by inhibiting FSTL-1 expression.We established puncture-induced model wild-type and FSTL-1+/- mice collected discs (IVDs) from the mice. Safranin O staining used detect cartilage loss IVD tissue, HE morphological changes tissue. We measured expression...
Abstract Osteoporosis is a systemic metabolic bone disorder for which inflammatory cytokines play an important role. To develop new osteoporosis treatments, strategies improving the microenvironment osteoblast and osteoclast balance are needed. Tumor necrosis factor-α (TNF-α) plays role in initiation development of osteoporosis. Atsttrin engineered protein derived from growth factor, progranulin (PGRN). The present study investigates whether affects formation formation. Here we show inhibits...