A5026711433- Enzyme Catalysis and Immobilization
- Microbial Community Ecology and Physiology
- Traffic control and management
- Autonomous Vehicle Technology and Safety
- Advanced MEMS and NEMS Technologies
- Microfluidic and Bio-sensing Technologies
- Polar Research and Ecology
- Electrowetting and Microfluidic Technologies
- Mechanical and Optical Resonators
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Vehicle Dynamics and Control Systems
- Microfluidic and Capillary Electrophoresis Applications
- Traffic Prediction and Management Techniques
- SARS-CoV-2 and COVID-19 Research
- Geophysics and Sensor Technology
- Protist diversity and phylogeny
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Biocrusts and Microbial Ecology
- Electric and Hybrid Vehicle Technologies
- Hearing, Cochlea, Tinnitus, Genetics
- Metabolism and Genetic Disorders
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
Polar Research Institute of China
2016-2025
Ministry of Natural Resources
2020-2025
Jilin University
2012-2024
Jimei University
2024
State Key Laboratory of Automotive Simulation and Control
2018-2024
Shanghai Jiao Tong University
2022-2024
Hefei University
2022-2024
University of Alabama at Birmingham
2007-2024
Birmingham VA Medical Center
2020-2024
Inner Mongolia People's Hospital
2016-2023
Abstract Predator-prey interactions play important roles in the cycling of marine organic matter. Here we show that a Gram-negative bacterium isolated from sediments ( Pseudoalteromonas sp. strain CF6-2) can kill Gram-positive bacteria diverse peptidoglycan (PG) chemotypes by secreting metalloprotease pseudoalterin. Secretion enzyme requires Type II secretion system. Pseudoalterin binds to glycan strands Gram positive bacterial PG and degrades peptide chains, leading cell death. The released...
The development of an effective and durable SARS-CoV-2 vaccine is essential for combating the growing COVID-19 pandemic. spike (S) glycoprotein main target neutralizing antibodies elicited during virus infection or following vaccination.
Alginate lyases play important roles in alginate degradation the ocean. Although a large number of have been characterized, little is yet known about those extremely cold polar environments, which may unique mechanisms for environmental adaptation and degradation. Here, we report characterization novel PL7 lyase AlyC3 from
To obtain chitinase-producing microorganisms with high chitinolytic activity at low temperature, samples collected from Fildes Peninsula in Antarctica were used as sources for bioprospecting of microorganisms. A cold-adapted strain, designated GWSMS-1, was isolated marine sediment and further characterized Pseudomonas. improve the chitinase production, one-factor-at-a-time orthogonal test approaches adopted to optimize medium components culture conditions. The results showed that highest...
The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in viral membrane to enter host cells. S is major target for neutralizing antibodies elicited by natural infection and vaccines. Approximately 35% consists carbohydrate, which can influence virus infectivity susceptibility antibody inhibition. We found that virus-like particles produced coexpression S, M, E, N proteins contained glycoproteins were extensively modified complex carbohydrates....
This paper presents a detailed study on the transient response and stability of automatic gain control (AGC) with proportion-integral (PI) controller for MEMS vibratory gyroscope, which constructs closed-loop system to make gyroscope achieve constant amplitude vibration at its resonant frequency. The nonlinear mathematical model is established by applying averaging linearization method, evaluated through numerical simulations. convergence characteristics whole loop are investigated using...
One-dimensional ZnO decorated with metal nanoparticles has received much attention in the field of ultraviolet light detection because its high photosensitivity and fast response, while how to form effective metal-ZnO heterostructures cost efficiently is still development. We report an efficient well-controlled method Au-ZnO by two-step dielectrophoretic assembly. First, nanowires dispersed deionized water were assembled dielectrophoretically a planar microelectrode system. To control number...
For intelligent four‐wheel‐drive (4WD) electric vehicle (EV), the speed can be planned and controlled for energy saving based on slope information of road ahead. To reduce calculation load optimisation algorithm, model predictive control (MPC) method is formulated time horizon in this study. Furthermore, a fast gradient tool‐GARMPC used to solve problem. First, longitudinal dynamics 4WD EV distance established information, respectively. Second, MPC problem time‐discrete solved by GARMPC...
When a permanent magnet synchronous motor runs at low speed, the inverter will output discontinuous current and generate torque ripple; when is high large amount of stator harmonic generates, which affects its speed following ability stability. To ensure fast smooth switching in full domain, this paper proposes nonsingular terminal sliding mode control PMSM based on improved exponential reaching law. Firstly, law composed state variables power terms surface functions. The function designed...
Metal nanoparticles in a liquid suspension can be assembled dielectrophoretically (DEP) into nanoparticle chains, which serve as electrical functional microwires connecting isolated and conductive elements to an electrode pair, used wet electronics, bioelectronics, biochemical sensors. The frequency-dependent morphology of these chains between pair has even been attributed the decreasing magnitude alternating current electroosmosis (ACEO) flow velocity with driving frequency. For instance,...
To characterize a robust NAD(+) -dependent formate dehydrogenase firstly obtained from nonmethylotroph, Bacillus sp. F1.The F1 (BacFDH) gene was cloned by TAIL-PCR and heterologous expressed in Escherichia coli. BacFDH stable at temperatures below 55°C, the half-life 60°C determined as 52·9 min. This enzyme also showed broad pH stability retained more than 80% of activities after incubating buffers with different ranging 4·5 to 10·5 for 1 h. The activity significantly enhanced some metal...
Traveling-wave electroosmotic (TWEO) pumping arises from the action of an imposed traveling-wave (TW) electric field on its own induced charge in diffuse double layer, which is formed top electrode array immersed electrolyte solutions. Such a traveling can be merely realized practice by discrete upon corresponding voltages correct phase are imposed. By employing theory linear and weakly nonlinear double-layer charging dynamics, physical model incorporating both surface capacitance layer...
Wheel odometry is a simple and low-cost localization technique that can be used for in GNSS-deprived environments; however, its measurement accuracy affected by many factors, such as wheel slip, wear, tire pressure changes, resulting unpredictable variable errors, which turn affect positioning performance. To improve the performance of odometry, this study developed error prediction model based on transformer neural network to learn uncertainty accurately predict error. Driving condition...
ABSTRACT SARS-CoV-2, a betacoronavirus, is the cause of COVID-19 pandemic. The SARS-CoV-2 spike (S) glycoprotein trimer mediates virus entry into host cells and cytopathic effects. We studied contribution several S features to these functions, focusing on those that differ among related coronaviruses. Acquisition furin cleavage site by decreased stability infectivity, but greatly enhanced ability form lethal syncytia. Notably, D614G change found in globally predominant strains restored...
Abstract. The diversity, co-occurrence patterns, and assembly processes of microbial eukaryotes (0.2–20 µm) in Antarctic freshwater lakes are not well understood, despite their wide distribution ecological importance. This study used Illumina high-throughput sequencing to investigate the eukaryotic communities five on Fildes Peninsula over three summer seasons. A total 28 phyla were detected, with phytoplankton occupying highest percentage sequences (accounting for up 98 %). dominant taxa...
Xylans are polysaccharides composed of xylose and include β1,4-xylan, β1,3-xylan, β1,3/1,4-mixed-linkage xylan (MLX). MLX is widely present in marine red algae constitutes a significant organic carbon the ocean. Xylanases hydrolase enzymes that play an important role degradation. While variety β1,4-xylanases β1,3-xylanases involved degradation β1,4-xylan β1,3-xylan have been reported, no specific enzyme has yet identified degrades MLX. Herein, we report characterization new MLX-specific...
Since the autonomous vehicle is intended to simulate a human driver's behaviour, it very helpful introduce driver model design of controller. The intention this paper exhibit approaches building longitudinally and laterally integrated methods applying in control. A series simulations undertaken typical driving conditions such as double lane change, speed or position following straight line, braking-in-turn, high-speed U turn, etc. During entire course simulations, has demonstrated great...
A novel β-1,3-galactosidase, designated as MaBGA (β-galactosidase from Marinomonas sp. BSi20414), was successfully purified to homogeneity BSi20414 isolated Arctic sea ice by ammonium sulfate precipitation and anion exchange chromatography, resulting in an 8.12-fold increase specific activity 9.9% recovery total activity. displayed its maximum at pH 6.0 60 °C, maintained least 90% of initial over the range 5.0-8.0 after incubating for 1 h. It also exhibited considerable thermal stability,...