ABSTRACT A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human syncytial virus (HRSV); parainfluenza 1, 2, 3 (HPIV1, -2, -3, respectively); influenza B were incorporated into a single standard format. The optimized panel used test 470 obtained 462 children hospitalized with acute illness that had been...

Foot-and-mouth disease virus non-structural protein 3A plays important roles in replication, virulence and host-range; nevertheless little is known on the interactions that this can establish with different cell components. In work, we have performed vivo dynamic studies from cells transiently expressing green fluorescent (GFP) fused to complete (GFP3A) versions including mutations. The results revealed presence of a mobile fraction GFP3A, which was found increased most mutants analyzed,...

Small proteins perform a diverse array of functions, from microbial competition, to endocrine signaling, building biomaterials. Microbial systems that can produce recombinant small enable discovery new effectors, exploration sequence activity relationships, and have the potential for in vivo delivery. However, we lack simple controlling small-protein secretion Gram-negative bacteria. Microcins are antibiotics secreted by bacteria inhibit growth neighboring microbes. They exported cytosol...

ABSTRACT Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in replication, virulence, and host range. In other picornaviruses, homodimerization of has been shown to be relevant for its biological activity. this work, FMDV was evidenced by an situ fluorescent ligation assay. A molecular model the protein, derived from nuclear magnetic resonance (NMR) structure poliovirus predicted a hydrophobic interface spanning residues 25 44 as main determinant...

Abstract We report here transport of the Epidermal Growth Factor Receptor (EGFR), Insulin Receptor, 7-pass transmembrane receptor Smoothened, and 13-pass Sodium-iodide symporter to extracellular vesicles (EVs) for structural functional studies. Mass spectrometry confirmed transported proteins as most abundant in EV membranes, presence many receptor-interacting demonstrates utility EVs characterizing membrane protein interactomes. Cryo-electron tomography EGFR-containing reveals that EGFR...

We performed a comparative analysis of the internalization mechanisms used by three viruses causing important vesicular diseases in animals. Swine disease virus (SVDV) was inhibited treatments that affected clathrin-mediated endocytosis and required traffic through an endosomal compartment. SVDV particles were found clathrin-coated pits electron microscopy colocalized with markers early endosomes confocal microscopy. infectivity significantly drugs raised pH. When compared to foot-and-mouth...

The Epidermal Growth Factor Receptor (EGFR) is a Tyrosine Kinase that mediates cell proliferation and differentiation events during development maintenance of complex organisms. Formation specific, ligand-dependent EGFR dimers key step in stimulating signaling, crystal structures active, dimeric forms isolated extracellular regions kinase domains have revealed much about how dimer interactions regulate activity. nature role the transmembrane region regulating activity remains less clear,...

Nonstructural protein 3A is involved in relevant functions foot-and-mouth disease virus (FMDV) replication. FMDV can form homodimers and preservation of the two hydrophobic α-helices (α1 α2) that stabilize dimer interface essential for In this work, small peptides mimicking residues were used to interfere with dimerization thus gain insight on its biological function. The α1, α2 spanning α-helices, α12, impaired vitro formation a peptide containing effect being higher α12. To assess...

Receptor Tyrosine Kinases (RTKs) comprise a diverse group of cell-surface receptors that mediate key signaling events during animal development and are frequently activated in cancer. We show here deletion the extracellular regions 10 RTKs representing 7 RTK classes or their substitution with dimeric immunoglobulin Fc region results constitutive receptor phosphorylation but fails to result downstream effectors Erk Akt. Conversely, Epidermal Growth Factor (EGFR) increases effector response...

Swine vesicular disease virus (SVDV) is a porcine pathogen and member of the species Enterovirus B within Picornaviridae family. Brefeldin A (BFA) an inhibitor guanine nucleotide exchange factors Arf proteins that induces Golgi complex disassembly alters cellular secretory pathway. Since BFA has been shown to inhibit RNA replication different enteroviruses, including SVDV, we have analyzed effect golgicide (GCA), another disrupting drug, on SVDV multiplication. GCA similarly inhibited...

Microcins are peptide antibiotics secreted by Gram-negative bacteria that inhibit the growth of neighboring microbes. They exported from cytosol to environment in a one-step process through specific type I secretion system (T1SS). While rules governing export natural or non-native substrates have been resolved for T1SSs secrete large proteins, relatively little is known about substrate requirements peptides microcins. Here, we investigate prototypic microcin V T1SS Escherichia coli and show...

Abstract Severe burns are associated with massive tissue destruction and cell death where nucleus histones other damage-associated molecular patterns released into the circulation contribute to pathogenesis of multiple organ dysfunction. Currently, there is limited information regarding pathophysiology extracellular following burns, mechanisms underlying histone-induced vascular injury not fully understood. In this study, by comparing blood samples from healthy donors burn patients, we...

Estudiar el efecto de la introducción en un hospital del Insalud analgesia epidural a demanda Un total 207 nulíparas con feto único término que parieron 1997, y 198 lo hicieron 1999-2000. Se comparan los dos grupos y, aisladamente, partos ambos No hemos observado cambios frecuencia operatorios o morbilidad neonatal, aunque se ha prolongado significativamente parto aumento uso oxitocina fiebre intraparto grupo más reciente. Las epidurales restringidas asociaban prolongado, dosis mayor empleo...

We report a case of bilateral acute iris transillumination (BAIT) syndrome caused by an overdose oral moxifloxacin in Hispanic female patient with no previous respiratory viral infection. A 56-year-old history ocular illness was referred to our glaucoma service manage her microcystic edema, swelling, and refractory hypertension. Her systemic symptoms, including progressively worsening pain, severe photophobia, blurred vision, nausea, vomiting, started 14 days after accidental moxifloxacin....