As a kind of potent stimulus, lipopolysaccharide (LPS) has the ability to cause cell damage by activating toll-like receptor(TLR)4, then nuclear factor kappa B (NF-κB) translocates into nucleus and changes expression related inflammatory genes. Baicalin is extracted from Radix Scutellariae, which possesses anti-inflammation, antioxidant antibacterial properties. However, effects it on LPS-induced liver inflammation have not been fully elucidated. This study aims investigate anti-inflammatory...

Hypericum perforatum L., also known as Saint John's Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of hypericum L. on infectious bronchitis virus (IBV) were evaluated in vitro vivo first time. The results experiments confirmed that component was ethyl acetate extraction section (HPE), showed treatment with HPE significantly reduced relative mRNA expression titer IBV, positive green immunofluorescence signal IBV...

It is well understood that liver cytochrome p450 enzymes are responsible for AFB1 bioactivation. While, phase-II regulated by the transcription factor nuclear factor-erythroid-2-related 2 (Nrf2) involved in detoxification of AFB1. In this study, we explored potential curcumin to prevent AFB1-induced injury modulating phase-I and along with Nrf2 bioactivation detoxification. Arbor Acres broiler were divided into four groups including control group (G1; fed only basal feed), alone-treated (G2;...

Cytochrome P450 enzymes are often responsible for the toxic and carcinogenic effects of toxicants, such as aflatoxin B1 (AFB1). The human hepatic CYP2A6 enzyme mediates oxidative metabolism several procarcinogens. In this study, we characterized a partial sequence gene from Arbor Acres (AA) broiler studied its role in AFB1 bioactivation. Moreover, effect curcumin on is illustrated. Six groups AA were treated 28 days including control group (fed only basal diet), alone-treated (450 mg/kg...

Aflatoxin B1 (AFB1) is very harmful for broiler production and public health. The water-soluble castoff in gluten production, i.e., the substances of wheat (WSW) that contains 14% pentosan has positive effect on animal nutrient absorption, immunity, antioxidation. Our study aims to investigate preventive effects WSW against AFB1-induced liver injury. One day-old Arbor Acres broilers were randomly separated 4 groups were, respectively, fed with control diet, diet 5 mg/kg AFB1 standard,...

Abstract Infectious bronchitis virus (IBV) is a pathogenic coronavirus with high morbidity and mortality in chicken breeding. Macrophages normal biofunctions are essential for host immune responses. In this study, the HD11 macrophage cell line peripheral blood mononuclear cell-derived macrophages (PBMCs-Mφ) were infected IBV at multiplicity of infection (MOI) 10. The dynamic changes their biofunctions, including viability, pathogen elimination function, phagocytic ability, gene expressions...

BACKGROUND Osteoporosis is associated with 17β-estradiol deficiency. The G protein-coupled receptor 30 (GPR30) known to be an estrogen-responsive receptor, but its role in the degradation of mitochondria osteoblasts by autophagy, or mitophagy, remains unclear. aim this vitro study was evaluate effects 17β-estradiol, GPR30, and signaling pathway, on mitophagy murine MC3T3-E1 osteoblast cell line. MATERIAL AND METHODS In line, cells were treated G15, a selective GPR30 antagonist, U0126,...

In this study, we investigated the mechanism underlying age-related susceptibility in broilers to aflatoxin B1 (AFB1). The results showed that AFB1 induced significant changes serum alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate (AST), gamma-glutamyl transferase (GGT) activity & liver superoxide dismutase (SOD), malonaldehyde (MDA), glutathione peroxidase (GSH-Px) and S-transferase (GST) at day 7, 21 42 relative control group. However, AFB1-induced biochemical...

Baicalin (BA) is a kind of flavonoid that isolated from Scutellaria baicalensis Georgi, which has been verified to have hepatoprotective effects in some diseases. However, the role BA acute hepatic injury induced by arsenic trioxide (ATO) remains unclear. The aim this study was investigate protective action on ATO and probe its possible mechanism. Mice were pretreated with (50, 100 mg/kg) gavage. After 7 h, (7.5 injected intraperitoneally induce liver injury. days treatment, serum specimens...

6-Gingerol (6G) is the main phenolic active component of ginger, which has a certain protective effect against liver injury induced by drugs, and chemical pollutants. However, mechanism protection 6G arsenic trioxide (ATO)-induced remains unclear. The network pharmacology animal experiment results respectively indicated that oxidative stress inflammatory responses were closely related to ATO-induced injury. can alleviate stress. Furthermore, attenuate release pro-inflammatory factors ATO...

Introduction: Corydalis yanhusuo total alkaloids (CYTA) are the primary active ingredients in , known for their analgesic and cardioprotective effects. However, mechanisms underlying treatment of Myocardial ischemia (MI) with CYTA have not been reported. The purpose this study was to explore protective effect on MI its related mechanisms. Methods: A network pharmacology employed shed light targets CYTA’s action MI. against hypoxia damage evaluated H9c2 cells. Furthermore, effects L-type Ca...

Liquiritin (LIQ) is a flavonoid known for its cardioprotective properties, extracted from <em>Glycyrrhiza</em> <em>uralensis</em> Fisch. The purpose of the present study was to investigate protective mechanism LIQ against hypoxia/reoxygenation (H/R) injury through <em>in</em> <em>vitro</em> experiments, with goal enhancing pharmacological effects. Initially, network pharmacology employed explore targets and mechanisms LIQ. Subsequently, an H/R model established using H9c2 cells. Potential...

To investigate the role of hepatic 18-carbon fatty acids (FA) accumulation in regulating CYP2A5/2A6 and significance Nrf2 process during hepatocytes steatosis, Nrf2-null wild type mice fed with high-fat diet (HFD), silenced or over expressed HepG2 cells administered FA were used. HE Oil Red O staining used for pathological examination. The mRNA protein expressions measured real-time PCR Western blot. results showed that CYP2A5 expression levels increased HFD accompanied accumulation. was...

Magnesium isoglycyrrhizinate (MgIG) has anti-inflammatory, antioxidative, antiviral and anti-hepatotoxic effects. However, protective effects of MgIG against renal damage caused by arsenic trioxide (ATO) have not been reported. The present study aimed to clarify the function on kidney damaged induced ATO. Other than control group treated with alone, mice were injected intraperitoneally ATO (5 mg/kg/day) for 7 days establish a mouse model damage. On 8th day, blood tissue collected...

The prevention and treatment of cerebral ischemia/reperfusion injury has become a key link in the ischemic cerebrovascular diseases. In this study, Wistar rats were randomly divided into Sham, low-dose ginsenoside (L-CK), high-dose (H-CK) nimodipine groups, L-CK, H-CK, groups intragastrically given corresponding agents successively once day for 15 days before surgery. At 1 h after last administration, rat model was established by suture-occluded method effects CK on neurological scores,...

Abstract Severe burns are associated with massive tissue destruction and cell death where nucleus histones other damage-associated molecular patterns released into the circulation contribute to pathogenesis of multiple organ dysfunction. Currently, there is limited information regarding pathophysiology extracellular following burns, mechanisms underlying histone-induced vascular injury not fully understood. In this study, by comparing blood samples from healthy donors burn patients, we...