A5019751830- Cardiomyopathy and Myosin Studies
- Corneal surgery and disorders
- Proteoglycans and glycosaminoglycans research
- Muscle Physiology and Disorders
- Collagen: Extraction and Characterization
- Force Microscopy Techniques and Applications
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Cardiovascular Effects of Exercise
- Ocular Surface and Contact Lens
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- Retinal Diseases and Treatments
- Protease and Inhibitor Mechanisms
- Retinal and Macular Surgery
- Epilepsy research and treatment
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Proteins in Food Systems
- Polyomavirus and related diseases
- Viral Infections and Immunology Research
- Molecular Biology Techniques and Applications
- Advanced Electron Microscopy Techniques and Applications
- Enzyme Structure and Function
- Physiological and biochemical adaptations
Cardiff University
2015-2025
University of Wales
2025
Imperial College London
2000-2018
NIHR Imperial Biomedical Research Centre
2003-2007
Chicago Zoological Society
1988
University of Illinois Urbana-Champaign
1988
Jackson Laboratory
1988
University of Pennsylvania
1988
Myosin binding protein C (MyBP-C) is a component of the thick filament striated muscle. The importance this revealed by recent evidence that mutations in cardiac gene are major cause familial hypertrophic cardiomyopathy. Here we investigate distribution MyBP-C A-bands and skeletal muscles compare to A-band structure muscle MyBP-C-deficient mice. We have used novel averaging technique obtain axial density electron micrographs well-preserved specimens. show very similar, with length 1.58 ±...
Significance The cornea is a specialized connective tissue assembled as remarkably ordered array of superimposed collagenous lamellae, and their component collagen fibrils, essential for optical transparency. Surprisingly, the mechanisms involved in deposition this unique structure are still not fully understood. Here we have used correlative microscopy techniques, including innovative methods serial block face scanning electron microscopy, to observe sequence corneal matrix formation...
The human glomerulus is the primary filtration unit of kidney, and contains Glomerular Filtration Barrier (GFB). GFB had been thought to comprise 3 layers - endothelium, basement membrane podocyte foot processes. However, recent studies have suggested that at least two additional contribute function GFB, endothelial glycocalyx on vascular side, sub-podocyte space urinary side. To investigate structure these difficult as it requires three-dimensional reconstruction delicate sub-microscopic...
Background/aims The antiseizure medication, vigabatrin, is associated with visual field loss (VAVFL). However, the fields can be challenging to interpret due unfamiliarity characteristics of defect and/or difficulty in obtaining a reliable examination, particularly patients cognitive limitations epilepsy. Two machine-learning pattern recognition algorithms were developed identify VAVFL, objectively. Methods adhered European Medicines Agency-approved protocol for detection VAVFL (Three Zone...
Please cite this paper as : Arkill KP, Neal CR, Mantell JM, Michel CC, Qvortrup K, Rostgaard J, Bates DO, Knupp C, Squire JM. 3D reconstruction of the glycocalyx structure in mammalian capillaries using electron tomography. Microcirculation 19: 343–351, 2012. Abstract Objective: Visualising molecular strands making up lumen small blood vessels has proved to be difficult conventional transmission microscopy techniques. Images obtained from tissue stained a variety ways have revealed...
The role of Decorin in organising the extracellular matrix was examined normal human corneas and from patients with Congenital Stromal Corneal Dystrophy (CSCD). In CSCD, corneal clouding occurs due to a truncating mutation (c.967delT) decorin (DCN) gene. Normal protein truncated one were reconstructed silico using homology modelling techniques explore structural changes diseased protein. CSCD specimens also 3-D electron tomography Small Angle X-ray diffraction (SAXS), image...
Inverted papillomas of the skin occurred in five dogs. Lesions were 1–2 cm, circumscribed, flask-like structures below level surrounding normal skin. Walls consisted hyperplastic epidermis, forming thin papillary projections on fibrovascular stalks. Cells stratum granulosum had clear cytoplasm, numerous keratohyalin-like granules various sizes, and poorly defined intranuclear inclusions. These cells stained positively for papillomavirus group-specific antigens by both...
To better understand the molecular mechanism involved in retrovirus ts1-induced paralytic disease mice, we constructed a panel of recombinant viruses between ts1 and wild-type Moloney murine leukemia virus (MoMuLV) MoMuLV-TB, strain MoMuLV. These were an attempt to identify sequence(s) genome which contains critical mutation(s) responsible for neurovirulence ts1. Two functionally distinct sequences found be its paralytogenic ability. One these sequences, 0.77-kilobase-pair XbaI-BamHI...
ts1 and ts7, two temperature-sensitive mutants of Moloney murine leukemia virus strain TB induce hind-limb paralysis in 100% CFW/D mice injected. These paralytogenic also share a defect their inability to process the env precursor protein, Pr80env, at restrictive temperature. To identify mutation(s) genomes which cause Pr80env efficiently confer ability instead lymphoma, we constructed chimeric between or virus. We identified 3.9-kilobase-pair HindIII-PstI sequence from nucleotides 4895...
The program HELIX has been devised as a simple aid to understanding the origin and appearance of fibre diffraction patterns from helical structures. Helices are common preferred conformations in both natural synthetic macromolecules ( e.g. DNA, α-helices, polysaccharides, polymers), occur frequently extended macromolecular aggregates actin filaments, myosin microtubules, amyloid filaments etc. ). For this reason, way visualizing kinds that these structures can give should have educational...