A5054838858- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Genomics and Chromatin Dynamics
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Advanced X-ray and CT Imaging
- Radiation Dose and Imaging
- RNA modifications and cancer
- Gastrointestinal Tumor Research and Treatment
- Welding Techniques and Residual Stresses
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Medical Research and Treatments
- Medical Imaging Techniques and Applications
- Ubiquitin and proteasome pathways
- Cardiac tumors and thrombi
- Vascular Malformations and Hemangiomas
- Wound Healing and Treatments
- Plant Molecular Biology Research
- Kruppel-like factors research
- Diverticular Disease and Complications
- High Altitude and Hypoxia
- Heart Rate Variability and Autonomic Control
- Mechanical Circulatory Support Devices
- Coronary Artery Anomalies
Chongqing Medical University
2025
Children's Hospital of Chongqing Medical University
2025
Chongqing Maternal and Child Health Hospital
2025
China International Science and Technology Cooperation
2025
Jiangsu Province Blood Center
2025
NYU Langone Health
2025
Jilin University
2013-2024
Shanghai Institute of Technology
2024
First Hospital of Jilin University
2013-2024
National Institute on Aging
2015-2024
The contribution of three-dimensional genome organization to physiological ageing is not well known. Here we show that large-scale chromatin reorganization distinguishes young and old bone marrow progenitor (pro-) B cells. These changes result in increased interactions at the compartment level reduced within topologically associated domains (TADs). gene encoding Ebf1, a key cell regulator, switches from A with age. Genetically reducing Ebf1 recapitulates some features pro-B TADs are most age...
Keloid is a fibrous benign tumor of the skin caused by increased fibroblast proliferation and overproduction extracellular matrix (ECM) in dermis. Several miRNAs exhibit critical roles regulating keloid development. This study aimed to investigate effects mechanisms miR-203 fibroblasts. The expression was detected qRT-PCR; cell viability measured MTT assay; BrdU invasion Transwell protein Western blot; target relationship between mRNA dual-luciferase assay. We found that significantly...
Background FFAs play an important role in the obesity management. Recent studies suggest that BFR training may also influence metabolic responses, including regulation of bloodstream. Understanding how acute resistance affects could provide valuable insights into effective interventions for improving health this population. Methods A two-arm randomized controlled design was employed. total 22 eligible subjects were randomly divided BFR-RE (n=11) and RE group (RE, n=11). Each participant...
In the clinical stage, suspected hemolytic plasma may cause hemolysis illness, manifesting as symptoms such heart failure, severe anemia, etc. Applying a deep learning method to images significantly improves recognition accuracy, so that this paper proposes quality detection model based on improved "You Only Look Once" 5th version (YOLOv5). Then presented in and evaluation system were introduced into datasets, the average accuracy of final classification reached 98.7%. The results...
Three-dimensional genome organization orchestrates recombination and transcription of immunoglobulin heavy chain (Igh) genes. The structure wild-type (WT) alleles includes a prominent architectural stripe that extends from cluster CTCF binding elements at the 3' end locus (3'CBE), suggesting interactions this with sequences throughout 2 Mb Igh TAD. Here we elucidate interplay between regulatory located in 3'Igh domain (260 kb) impact stripe. CTCF-lacking intronic enhancer, Eµ, promotes...
CCCTC-binding factor (CTCF) is essential for chromatin organization. CTCF interacts with endogenous RNAs, and deletion of its ZF1 RNA-binding region (∆ZF1) disrupts loops in mouse embryonic stem cells (ESCs). However, the functional significance CTCF-ZF1 RNA interactions during cell differentiation unknown. Using an ESC-to-neural progenitor (NPC) model, we show that crucial maintaining cell-type-specific loops. Expression CTCF-∆ZF1 leads to disrupted dysregulation genes within these loops,...
TCF1 plays a critical role in T lineage commitment and the development of αβ cells, but its γδ cell remains poorly understood. Here, we reveal regulatory axis where receptor (TCR) signaling controls expression through an E-protein-bound element Tcf7 locus, this regulates both effector fate. Indeed, level important setting threshold for modulates ability TCR to influence fate adoption by progenitors. This finding provides mechanistic insight into how TCR-mediated repression E proteins...
Kainic acid (KA) treatment causes neuronal degeneration, which is a feature of Alzheimer's disease (AD) symptoms such as amyloid β-protein production and memory deficits. Inflammasomes are known to be critical for the progression AD. However, underlying mechanism by inflammasomes influence AD remains unknown. The present study investigated damaging effect KA on neurons focusing inflammasome-mediated signaling pathways. Assessments using cultured microglia mouse brains demonstrated that...
Conformation of antigen receptor gene loci spatially juxtaposes rearranging segments in the appropriate cell lineage and developmental stage. We describe a three-step pathway that establishes structure 2.8-Mb immunoglobulin heavy chain (IgH) locus pro-B cells. Each step uses different transcription factor leads to increasing levels structural organization. CTCF mediates one level compaction folds into several 250- 400-kb subdomains, Pax5 further compacts 2-Mb region encodes variable (VH)...
Posterior Nutcracker syndrome (NCS) is a rare anomaly in which the left renal vein passes behind aorta compresses it against vertebral column, restricting venous drainage of kidney.A 46 year-old lady presented with intermittent painless hematuria for 6 years. Urinalysis showed microscopic hematuria. An abdominal CT scan duplication retroaortic branch trapped between column and at level aortic bifurcation, suggestive posterior NCS. There were multiple small cortical cysts, sand-like stones...
Different mechanisms govern the first and second steps of IgH gene recombination.
Significance The prevailing dogma is that renewed mitogenic signaling essential to traverse G1 phase of the cell cycle after each division. B lymphocytes undergo multiple mitotic divisions, termed clonal expansion, expand antigen-specific cells mediate effective immunity. Here we demonstrate have undergone one division continue proliferate even in absence further signals. This mitogen-independent proliferation accompanied by an altered marked transcriptomic and proteomic features G2/M....
To examine the value of energetic-spectrum computed tomography (spectral CT) quantitative parameters in renal cell carcinoma (RCC) microvascular angiogenesis.The authors evaluated 32 patients with pathologically confirmed RCC who underwent triple-phase contrast-enhanced CT spectral imaging mode from January 2017 to December 2019. Quantitative include derived iodine concentration (IC) and water (WC) 120 keV monochromatic images. All specimens were including density (MVD), area (MVA) so on....
Loss-of-function studies have shown that transcription factor T cell factor-1 (TCF1), encoded by the Tcf7 gene, is essential for development in thymus. We discovered expression level regulated E box DNA binding proteins, independent of Notch, and regulates αβ γδ development. Systematic interrogation five protein elements (EPE1–5) enhancer region showed lineage-specific utilization. Specifically, loss-of-function analysis revealed only EPE3 plays a critical role supporting development, while...
<title>Abstract</title> <bold>Background: </bold>While deep learning image reconstruction(DLIR) has been applied successfully in thoracic, abdominal, and vascular examinations, its application low-dose renal CT protocols not previously explored. <bold>Purpose: </bold>To explore the value of DLIR reducing radiation dose improving quality contrast-enhanced compared with adaptive statistical iterative reconstruction Veo(ASIR-V). <bold>Material Methods:</bold> Methods: 129 disease patients...
Microreactors are essential for microchemical reactions owing to their high mass transfer efficiency, precise control of reaction time, easy amplification, and good safety performance. These characteristics provide several advantages, including shortened times enhanced chemical conversion rates, rendering microreactors particularly significant in production. In this study, a finite-rate model was developed the esterification monobutyl chlorophosphate (MCP) n-butanol microchannel reactor....
Malignant epithelioid hemangioendothelioma (EHE) is an uncommon and grave vascular tumor. EHE frequently angiocentric associated with a medium sized vessel, especially vein. No definite etiological associations have been ascribed to this tumor so far, except association oral contraceptives in of liver.A 47 year old man presented the complaint intermittent black stool over past two weeks. Occasionally, he experienced pain left lower abdomen. On Computed Tomography (CT), it showed...
Abstract
Abstract The canonical view of the cell cycle posits that G1 progression signals are essential after each mitosis to enter S phase. A subset tumor cells bypass this requirement and progress next division in absence continued signaling. B T lymphocytes adaptive immune system undergo a proliferative burst, termed clonal expansion, generate pools antigen specific for effective immunity. There is evidence rules lymphocyte digress from model. Here we show sustain several rounds...
Antigen receptors (AgRs) expressed on B and T cells provide the adaptive immune system with ability to detect numerous foreign antigens. Epigenetic features of cell receptor (BCR) (TCR) genes were previously studied in lymphocytes, but little is known about their epigenetic other cells. Here, we explored histone modifications transcription markers at BCR TCR loci lymphocytes (pro-B, DP cells, mature CD4<sup>+</sup> cells), compared embryonic stem (ES) neurons. In exhibited active...