A5101892203- Parkinson's Disease Mechanisms and Treatments
- Genetic Neurodegenerative Diseases
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Cholinesterase and Neurodegenerative Diseases
- Endoplasmic Reticulum Stress and Disease
- Bone Tissue Engineering Materials
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Computational Drug Discovery Methods
- Lymphatic System and Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Pharmacological Effects of Natural Compounds
- Angiogenesis and VEGF in Cancer
- 3D Printing in Biomedical Research
- Sphingolipid Metabolism and Signaling
- Kruppel-like factors research
- Nuclear Receptors and Signaling
- Neurological diseases and metabolism
- Additive Manufacturing and 3D Printing Technologies
- Ubiquitin and proteasome pathways
- 14-3-3 protein interactions
- Tryptophan and brain disorders
- Electrospun Nanofibers in Biomedical Applications
- Fibroblast Growth Factor Research
Chang Gung University
2015-2025
Chang Gung Memorial Hospital
2015-2025
National Taiwan University Hospital
2003-2024
National Taiwan University
2008-2024
National Taiwan Normal University
2006-2022
Taipei Medical University
2021
National Yang Ming Chiao Tung University
2016-2020
Linkou Chang Gung Memorial Hospital
2016-2020
National Yang Ming University Hospital
2018-2020
National Taipei University
2016-2019
For stem cell differentiation, the microenvironment can play an important role, and hydrogels provide a three-dimensional to allow native growth in vitro. A challenge is that cell's differentiation be influenced by matrix stiffness. We demonstrate low-toxicity method create different stiffness matrices, using photopolymerizable gelatin methacrylate (GelMA) hydrogel cross-linked blue light (440 nm). The porosity of GelMA easily modified altering its concentration. used human bone marrow...
Amyloid β (Aβ) plays a major role in the neurodegeneration of Alzheimerâs disease (AD). The accumulation misfolded Aβ causes oxidative stress and inflammatory damage leading to apoptotic cell death. Traditional Chinese herbal medicine (CHM) has been widely used treating neurodegenerative diseases by reducing neuroinflammation. We examined neuroprotective effect formulated CHM Shaoyao Gancao Tang (SG-Tang, made Paeonia lactiflora Glycyrrhiza uralensis at 1:1 ratio) AD mouse models. In...
Neuroinflammation and oxidative stress have been emerging as important pathways contributing to Parkinson's disease (PD) pathogenesis. In PD brains, the activated microglia release inflammatory factors such interleukin (IL)-β, IL-6, tumor necrosis factor (TNF)-α, nitric oxide (NO), which increase mediate neurodegeneration. Using 1-methyl-4-phenylpyridinium (MPP+)-activated human microglial HMC3 cells sub-chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD,...
Misfolding and accumulation of amyloid-β (Aβ) in the brains patients with Alzheimer's disease (AD) lead to neuronal loss through various mechanisms, including downregulation eukaryotic elongation factor 2 (EEF2) protein synthesis signaling. This study investigated neuroprotective effects indole coumarin derivatives on Aβ folding EEF2 signaling using SH-SY5Y cells expressing Aβ-green fluorescent (GFP) reporter. Among tested compounds, two (NC009-1, -6) (LM-021, -036) effectively reduced...
The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen PTFE membranes. PCL-based PUs were prepared by polymerization polycaprolactone (PCL) diol 1,6-hexamethylene diisocyanate (HDI) different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. chemical,...
Parkinson's disease (PD) is a common neurodegenerative characterized by progressive loss of dopaminergic (DAergic) neurons in ventral brain. A disaccharide trehalose has demonstrated the potential to mitigate DAergic models for PD. However, rapidly hydrolyzed into glucose trehalase intestine, limiting its clinical practice. Here we investigated neuroprotective potentials two trehalase-indigestible analogs, lactulose and melibiose, sub-chronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine...
Parkinson’s disease (PD) is a common neurodegenerative disorder characterized by the loss of dopaminergic (DAergic) neurons and presence α-synuclein-containing Lewy bodies. The unstructured α-synuclein forms insoluble fibrils aggregates that result in increased reactive oxygen species (ROS) cellular toxicity PD. Neuroinflammation engaged microglia actively contributes to pathogenesis In this study, we showed VB-037 (a quinoline compound), glycyrrhetic acid pentacyclic triterpenoid),...
Trehalose, a chemical chaperone and mTOR-independent autophagy enhancer, has shown promise in models of Huntington's disease, Parkinson's disease tauopathies. In this study, two trehalase analogs, lactulose melibiose, were examined for their potentials spinocerebellar ataxia treatment. Using SCA3 ATXN3/Q75-GFP cell model, we found that the ATXN3/Q75 aggregation was significantly prohibited by melibiose because abilities to up-regulate autophagy. Meanwhile, reduced reactive oxygen species...
Alzheimer's disease (AD) and several neurodegenerative disorders known as tauopathies are characterized by misfolding aggregation of tau protein. Although studies have suggested the potential traditional Chinese medicine (TCM) treatment for diseases, role TCM in treating AD not been well explored.Tau protein was coupled to DsRed fluorophore fusing a pro-aggregation mutant repeat domain (ΔK280 tauRD) with DsRed. The ΔK280 tauRD-DsRed fusion gene then used generate Tet-On 293 SH-SY5Y cell...
Spinocerebellar ataxia (SCA) types 1, 2, 3, 6, 7, and 17 as well Huntington’s disease are a group of neurodegenerative disorders caused by expanded CAG repeats encoding long polyglutamine (polyQ) tract in the respective proteins. Evidence has shown that accumulation intranuclear cytoplasmic misfolded polyQ proteins leads to apoptosis cell death. Thus suppression aggregate formation is expected inhibit wide range downstream pathogenic events diseases. In this study, we established...
In polyglutamine (polyQ)-mediated disorders, the expansion of translated CAG repeats in disease genes result long polyQ tracts their respective proteins, leading to intracellular accumulation aggregated production reactive oxygen species, and cell death. The molecular chaperones act preventing protein misfolding aggregation, thus inhibiting a wide range harmful downstream events. circumstance autophagic pathway is induced degrade misfolded or proteins. this study, we used Flp-In 293/SH-SY5Y...
Neurofibrillary tangles formed from tau misfolding have long been considered one of the pathological hallmarks Alzheimer's disease (AD). The in AD correlates with clinical progression and inhibition or reversal may protect affected neurons.We generated 293 SH-SY5Y cells expressing DsRed-tagged pro-aggregation mutant repeat domain (ΔK280 tauRD ) to test indole/indolylquinoline derivatives for reducing neuroprotection.Four 10 tested displayed good misfolding-inhibitory effects on Tet-On cells....
Spinocerebellar ataxia (SCA) type 17 is an autosomal dominant caused by expanded polyglutamine (polyQ) tract in the TATA-box binding protein (TBP). Substantial studies have shown involvement of compromised mitochondria biogenesis regulator peroxisome proliferator-activated receptor gamma-coactivator 1 alpha (PGC-1α), nuclear factor erythroid 2-related 2 (NRF2), factor-Y subunit A (NFYA), and their downstream target genes pathogenesis polyQ-expansion diseases. The extracts Paeonia lactiflora...
Gelatin methacryloyl (GelMA) hydrogel is a photopolymerizable biomaterial widely used for three-dimensional (3D) cell culture due to its high biocompatibility. However, the drawback of GelMA poor mechanical properties, which may compromise feasibility biofabrication techniques. In this study, cell-laden composite with combination incorporating silanized hydroxyapatite (Si-HAp) and simple harmless visible light crosslinking system were developed. The incorporation Si-HAp into enhanced...
Abstract PPP2R2B, a protein widely expressed in neurons throughout the brain, regulates phosphatase 2A (PP2A) activity for microtubule‐associated tau and other substrates. Altered PP2A has been implicated spinocerebellar ataxia 12, Alzheimer's disease (AD), tauopathies. Through case‐control study reporter assay, we investigated association of PPP2R2B CAG repeat polymorphism with Taiwanese AD, essential tremor (ET), Parkinson's (PD), schizophrenia clarified functional implication this...
<h3>Background/aims</h3> Povidone–iodine (PI) is commonly used as a preoperative disinfectant; however, it has been shown to be cytotoxic. The present study was performed investigate the mechanism by which PI causes cell death. <h3>Methods</h3> Primary human corneal fibroblasts (HCF) and epithelial line (HCEC) were treated with 0.1–5% for 1 min. Cell morphology growth examined phase-contrast microscopy genomic DNA quantification. Cellular enzyme activities detected water-soluble tetrazolium...
Mutations in ATP13A2 have been reported to associate with Parkinson's disease (PD). This study investigates the contribution of genetic variants Taiwanese PD. cDNA fragments from 65 early onset PD (onset <50 years) were sequenced. The identified validated a cohort (n = 493) and ethnically matched controls 585). A novel heterozygous G1014S, located at conserved seventh transmembrane domain protein, was an patient, which absent 585 normal controls. Additionally, A746T found two patients four...