A5037304323- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Liver Disease Diagnosis and Treatment
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Genetic Associations and Epidemiology
- Diet and metabolism studies
- Nutrition and Health in Aging
- MicroRNA in disease regulation
- Biochemical Analysis and Sensing Techniques
- Epigenetics and DNA Methylation
- Lipid metabolism and disorders
- Cardiovascular Disease and Adiposity
- Genetic and phenotypic traits in livestock
- GDF15 and Related Biomarkers
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Birth, Development, and Health
- Apelin-related biomedical research
- Clusterin in disease pathology
- Alcohol Consumption and Health Effects
- Protease and Inhibitor Mechanisms
University Hospital Leipzig
2020-2025
German Center for Diabetes Research
2021-2023
Helmholtz Zentrum München
2022
Leipzig University
2015-2020
IFB Adiposity Diseases
2016-2020
Institut d'Investigació Sanitària Pere Virgili
2013-2016
Universitat Rovira i Virgili
2013-2016
Agència de Gestió d'Ajuts Universitaris i de Recerca
2014-2016
Hospital Universitari Joan XXIII de Tarragona
2013-2014
Autophagy genes' expression is upregulated in visceral fat human obesity, associating with obesity-related cardio-metabolic risk. E2F1 (E2F transcription factor 1) was shown cancer cells to transcriptionally regulate autophagy. We hypothesize that regulates adipocyte autophagy endocrine/metabolic dysfunction, thereby, representing non-cell-cycle function of this factor. protein (N=69) and mRNA (N=437) were elevated obese humans, correlating increased ATG5 (autophagy-related 5), MAP1LC3B/LC3B...
Abstract Background/Objectives Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of damage, from simple steatosis (SS) to cirrhosis. SS and non-alcoholic steatohepatitis (NASH) cannot be distinguished by clinical or laboratory features. Dysregulation the gut microbiota is involved in NASH pathogenesis. The aim this study was assess relationship between microbiota-derived metabolites degrees NAFLD; also, investigate whether these could included panel biomarkers....
(1) Adipsin is an adipokine that may link increased fat mass and adipose tissue dysfunction to obesity-related cardiometabolic diseases. Here, we investigated whether adipsin serum concentrations (AT) mRNA expression are related parameters of AT function, obesity type 2 diabetes (T2D). (2) Methods: A cohort 637 individuals with a wide range age body weight (Age: 18-85 years; BMI: 19-70 kg/m2) (n = 237) or without 400) T2D was analyzed for by ELISA visceral (VAT) subcutaneous (SAT) RT-PCR....
Objective Bone morphogenetic proteins (BMPs) are important regulators of adipogenesis and may play a role in obesity. In this study, the hypothesis that BMP2 is related to adipose tissue (AT) distribution obesity was tested. Methods serum concentration ( n = 439) Schnurri‐1 −2 mRNA expression were measured paired samples visceral subcutaneous AT from 547 individuals with wide range body mass index. addition, single nucleotide polymorphism rs979012 gene genotyped for subsequent association...
Lipid accumulation in the human liver seems to be a crucial mechanism pathogenesis and progression of non-alcoholic fatty disease (NAFLD). We aimed evaluate gene expression different acid (FA) metabolism-related genes morbidly obese (MO) women with NAFLD. Liver key related de novo FA synthesis (LXRα, SREBP1c, ACC1, FAS), uptake transport (PPARγ, CD36, FABP4), oxidation (PPARα), inflammation (IL6, TNFα, CRP, PPARδ) were assessed by RT-qPCR 127 MO normal histology (NL, n = 13), simple...
Specific miRNA expression profiles have been shown to be associated with nonalcoholic fatty liver disease (NAFLD). We examined the correlation between circulating levels and hepatic of miR122 miR33a/b*, key lipid metabolism-related gene clinicopathological factors obese women NAFLD. measured miR33a/b* in samples from 62 morbidly (MO), 30 moderately (ModO), eight normal-weight controls. MiR122 was analyzed by qRT-PCR. Additionally, miR33b* were 122 women. Hepatic increased MO compared ModO...
Since the emergence of coronavirus disease-19 (COVID-19) in 2019, it has been crucial to investigate causes severe cases, particularly higher rates hospitalization and mortality individuals with obesity. Previous findings suggest that adipocytes may play a role adverse COVID-19 outcomes people The impact vaccination infection on adipose tissue (AT) is currently unclear. We therefore analyzed 27 paired biopsies visceral subcutaneous AT from donors Leipzig Obesity BioBank have categorized into...
Abstract Objective Maternal hormonal status can have long-term effects on offspring metabolic health and is likely regulated via epigenetic mechanisms. We elucidated the of maternal thyroid hormones regulation leptin ( Lep ) transcription in adipose tissue (AT) subsequently investigated role DNA methylation at a upstream enhancer (UE) adipocyte biology. Results Pregnant mice treated with triiodothyronine (T3) produced reduced body weight, total fat mass, gonadal white (gWAT) mass 6 months...
TMEM18 is the strongest candidate for childhood obesity identified from GWASs, yet as most GWAS-derived obesity-susceptibility genes, functional mechanism remains elusive. We here investigate relevance of adipose tissue development and obesity. demonstrate that adipocyte expression downregulated in children with Functionally, downregulation impairs formation zebrafish human preadipocytes, indicating important differentiation vivo vitro. On molecular level, activates PPARG, particularly...
Abstract Background There is growing interest in the measurement of growth differentiation factor 15 (GDF-15) a range disorders associated with cachexia. We undertook studies to determine whether common histidine (H) aspartate (D) variant at position 202 pro-peptide (position 6 mature peptide) interfered its detection by 3 most commonly used immunoassays. Methods Three synthetic GDF-15-forms (HH homo-, HD hetero-, and DD-homodimers) were measured after serial dilution using Roche Elecsys®,...
ABSTRACT Objective Starvation‐induced depletion of fat stores in anorexia nervosa (AN) is known to be accompanied by alterations some circulating adipocytokines. We analyzed a panel adipocytokines women with AN compared normal‐weight controls and their relation the disease duration weight restoration. Method adipocytokine levels 28 patients 33 who were eating healthily. determined enzyme‐linked immunosorbent assay total high molecular (HMW) adiponectin, lipocalin‐2 (LCN2), leptin, tumor...
Objective The aim of this study was to analyse the expression crucial genes in fatty acid metabolism visceral (VAT) and subcutaneous (SAT) adipose tissue samples from morbidly obese women. Methods VAT SAT key 145 women (MO, BMI > 40 Kg/m 2 ) 18 normal weight control by RT‐PCR Western Blot analyzed. Results In SAT, levels related lipogenesis oxidation were significantly lower MO than controls. VAT, most lipogenic studied had similar cohort. Regarding inflammation, IL6 higher both tissues...
Recent studies report the effect of bariatric surgery on glycaemia control and prevention type-2-diabetes in obese patients. This study is about pathophysiological mechanisms associated to these changes.Circulating levels receptors tumor necrosis factor (TNF-RI, TNF-RII), visfatin, high molecular weight (HMW) adiponectin, C reactive protein (CRP) 30 morbidly women (body mass index, BMI>40 kg/m(2) ) 60 normal-weight controls (BMI>25 were analyzed. Morbidly studied at three time-points: before...
Novel pro-inflammatory and anti-inflammatory derivatives from adipose tissue, known as adipokines, act metabolic factors. The aim of this study was to analyse the secreted expression different adipo/cytokines in secretomes unstable carotid atherosclerotic plaque versus non-atherosclerotic mammary artery.We evaluated secretion levels adiponectin, visfatin, lipocalin-2, resistin, IL-6 TNFR2 by ELISA human cultured (n = 18) artery 13). We also measured visfatin serum patients suffering...
Background . Recent reports suggest a role for the endocannabinoid system in pathology of nonalcoholic fatty liver disease (NAFLD). The aim this study was to investigate relationship between expression cannabinoid (CB) receptor subtypes, CB1 and CB2, morbidly obese (MO) women with different histological stages NAFLD. Methods We analysed hepatic CB2 mRNA expression, genes involved lipid metabolism 72 MO women, subclassified by histology into normal (NL,<mml:math...
Data from recent studies conducted in rodent models and humans suggest that interleukin-17A (IL-17A) plays a role the induction of inflammation adipose tissue during obesity. The aim this study was to assess gene expression IL-17A morbidly obese patients. We used RT-PCR evaluate several adipo/cytokines visceral (VAT) subcutaneous (SAT) 10 normal-weight control women (BMI < 25 kg/m2) 30 (MO, BMI > 40 kg/m2). measured serum levels MO normal weight women. significantly higher VAT than SAT...
Because of the clinical significance carotid atherosclerosis, search for novel biomarkers has become a priority. The aim present study was to compare protein secretion profile atherosclerotic plaque (CAP, n = 12) and nonatherosclerotic mammary artery (MA, 10) secretomes. We used nontargeted proteomic approach that incorporated tandem immunoaffinity depletion, iTRAQ labeling, nanoflow liquid chromatography coupled high-resolution mass spectrometry. In total, 162 proteins were quantified,...
Recent reports suggest a role for the Patatin-like phospholipase domain-containing protein 3 (PNPLA3) in pathology of non-alcoholic fatty liver disease (NAFLD). Lipid deposition seems to be critical process pathogenesis NAFLD. The aim present work was evaluate association between PNPLA3 expression, key genes lipid metabolism, and presence NAFLD morbidly obese women. We used real-time polymerase chain reaction (PCR) analysis analyze hepatic expression metabolism-related 55 subjects with...
The purpose of this work was to evaluate the expression fatty acid metabolism-related genes in human adipose tissue from moderately obese women. We used qRT-PCR and Western Blot analyze visceral (VAT) subcutaneous (SAT) mRNA involved de novo synthesis (ACC1, FAS), oxidation (PPARα, PPARδ) inflammation (IL6, TNFα), normal weight control women (BMI < 25 kg/m2, n = 35) 30–38 55). In SAT, ACC1, FAS PPARα were significantly decreased compared controls. downregulation reported SAT more...
Background Non-alcoholic fatty liver disease (NAFLD) causes a wide spectrum of damage, ranging from simple steatosis to cirrhosis. However, (SS) and steatohepatitis (NASH) cannot yet be distinguished by clinical or laboratory features. The aim this study was assess the relationship between alpha-ketoglutarate degrees NAFLD in morbidly obese patients. Materials Methods We used gas chromatography-quadruple time-of-flight-mass spectrometry analysis quantify serum normal-weight subjects (n = 30)...
Objective This cohort study aimed to explore the relationship between Notch signaling pathway and degree of nonalcoholic fatty liver disease (NAFLD). Moreover, this intended investigate whether is related hepatic lipid metabolism Toll‐like receptors (TLRs). Methods used real‐time polymerase chain reaction analysis evaluate expression level all genes studied (Notch NOTCH1 , NOTCH2 NOTCH3 NOTCH4 transcription factors HES 1 HES5 Hes‐related repressor proteins HEY HEY2 ) in tissue from two...