Abstract Calf diarrhea is associated with enteric infections, and also provokes the overuse of antibiotics. Therefore, proper treatment represents a therapeutic challenge in livestock production public health concerns. Here, we describe ability fecal microbiota transplantation (FMT), to ameliorate restore gut microbial composition 57 growing calves. We conduct multi-omics analysis 450 longitudinally collected samples find that FMT-induced alterations (an increase family Porphyromonadaceae )...

Diabetic patients with a long disease duration usually accompanied complication such as diabetic retinopathy, but in some had no complication.We analyzed differences plasma metabolites according to the presence or absence of retinopathy (DR) type 2 (T2D) ≥ 15 years.A cohort 183 T2D was established. Their biospecimens and clinical information were collected accordance guidelines National Biobank Korea, Korean Diabetes Association. DR phenotypes subjects verified by ophthalmologic specialists....

Non-targeted metabolomic analysis of hot pepper (Capsicum annuum "CM334") was performed at six development stages [16, 25, 36, 38, 43, and 48 days post-anthesis (DPA)] to analyze biochemical changes. Distinct distribution patterns were observed in the changes metabolites, gene expressions, antioxidant activities by early (16-25 DPA), breaker (36-38 later (43-48 DPA) stages. In stages, glycosides luteolin, apigenin, quercetin, shikimic acid, γ-aminobutyric acid (GABA), putrescine highly...

The objective of this study was to isolate multifunctional bacteriocin-producing strains; characterize the expressed bacteriocin for control Listeria monocytogenes and vancomycin-resistant Enterococcus; evaluate safety studied explore their antifungal activity.Two Pediococcus strains were isolated from silage samples obtained an organic farm in Belogradchik, Bulgaria. identified by 16S rRNA sequencing analysis characterized as bacteriocins producers. Strong antimicrobial activity detected...

Nine varieties of pigmented rice (Oryza sativa L.) seeds that were black, red, or white used to perform metabolite profiling by using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and gas chromatography (GC) TOF-MS, measure antioxidant activities. Clear grouping patterns determined the color identified in principle component analysis (PCA) derived from UPLC-Q-TOF-MS. Cyanidin-3-glucoside, peonidin-3-glucoside, proanthocyanidin dimer,...

In general, greenhouse cultivation involves the rampant application of chemical fertilizers, with aim achieving high yields. Oversaturation mineral nutrients that aid plant growth, development, and yield may lead to abiotic stress conditions. We explore effects excess magnesium on tomato metabolism, as well fruit quality using non-targeted mass spectrometry (MS)-based metabolomic approaches. Tomato plants were subjected three different experiments, including (MgH), extremely (MgEH), a...

<b><i>Background:</i></b> Pregnancy-associated breast cancer (PABC) is rare and generally defined as diagnosed during pregnancy or within 1 year of delivery. The average ages marriage childbearing are increasing, PABC expected to also increase. This study intended increase understanding the characteristics PABC. <b><i>Methods:</i></b> A database 2,810 patients with when they were less than 40 years age was reviewed. clinicopathological factors...

This study was designed to determine the prevalence of depression among hemodialysis (HD) patients from urban hospitals in Korea, illustrate demographic factors and biomarkers associated with health-related quality life (HRQOL), demonstrate association between HRQOL.For this multicenter, cross-sectional study, 160 HD 3 university teaching local dialysis units Korea were enrolled. Korean Beck's inventory version Kidney Disease Quality Life short form, 1.3 (KDQOL-SFTM 1.3) used evaluate life,...

Irritable bowel syndrome (IBS) causes intestinal discomfort, gut dysfunction, and poor quality of life. This randomized, double-blind placebo-controlled trial evaluated the efficacy Lactiplantibacillus (Lp., formerly Lactobacillus) plantarum APsulloc 331261 (GTB1TM) from green tea leaves in participants with diarrhea-predominant irritable (IBS-D). Twenty-seven meeting Rome IV diagnostic criteria were randomized for GTB1 or placebo ingestion four weeks follow-up two weeks. The endpoints...

Metabolite profiling of three blueberry species (Vaccinium bracteatum Thunb., V. oldhamii Miquel., and corymbosum L.) was performed using gas chromatography-time-of-flight-mass spectrometry (GC-TOF-MS) ultraperformance liquid chromatography-quadrupole-time-of-flight-mass (UPLC-Q-TOF-MS) combined multivariate analysis. Partial least-squares discriminant analysis clearly showed metabolic differences among species. GC-TOF-MS revealed significant in amino acids, organic fatty sugars, phenolic...

Background and Purpose Thiazolidinediones, acting as PPAR‐γ ligands, reduce hepatic steatosis in humans animals. However, the underlying mechanism of this action remains unclear. The purpose study was to investigate changes metabolites lipids response treatment with thiazolidinedione pioglitazone an animal model obese Type 2 diabetes. Experimental Approach Male Otsuka Long‐Evans Tokushima Fatty (OLETF) rats were orally administered either vehicle (control) or (30 mg·kg −1 ) fed a high‐fat...

We used ultra-performance-liquid-chromatography with quadrupole-time-of-flight mass spectrometry to study the changes in metabolites mixture of Cudrania tricuspidata, Lonicera caerulea, and soybean (CLM) during fermentation. Additionally, antiobesity effects CLM fermented-CLM (FCLM) were studied based on analysis plasma from high-fat diet (HFD)-fed mice. The levels cyanidin glycosides luteolin, quercetin, derived L. caerulea decreased, whereas luteolin quercetin increased Isoflavone...

To investigate the pathophysiologic characteristics of diabetic complications, we identified differences in plasma metabolites subjects with type 2 diabetes (T2DM) or without macular edema (DME) and a disease duration > 15 years. An cohort older T2DM patients prolonged was established, clinical information biospecimens were collected following guidelines National Biobank Korea. DME phenotypes by ophthalmologic specialists. For metabolomics studies, propensity matched case control samples...

Summary Chronic respiratory diseases are part of accumulating health problems partly due to worldwide increase in air pollution. By their antimicrobial and immunomodulatory properties, some probiotics constitute promising alternatives for the prevention treatment chronic diseases. We have isolated Bacillus strains from Korean fermented foods selected three potentially probiotic (two subtilis one amyloliquefaciens ) based on safety, efficacy, activity against airborne pathogens properties...

Green tea supplementation has beneficial health effects. However, its underlying mechanisms, such as effects on modulating the intestinal microbiome and endogenous metabolome, particularly following short-term supplementation, are largely unclear. We conducted an integrative metabolomics study to evaluate of (7-day) green extract (GTE) or components, epigallocatechin gallate, caffeine, theanine, caecum microbiota caecum/skin metabolome in mice. Further, we established metabolome-microbiome...

Beech mushrooms (Hypsizygus marmoreus) are largely relished for their characteristic earthy flavor, chewy-texture, and gustatory nutritional properties in East Asian societies. Intriguingly, the aforementioned of beech mushroom can be subsumed under its elusive metabolome subtle transcriptome regulating various stages growth development. Herein, we carried out an integrated metabolomic transcriptomic profiling different sized across spatial components (cap stipe) to delineate signature...

Background Ezetimibe, which lowers cholesterol by blocking the intestinal transporter Niemann-Pick C1 like 1, is reported to reduce hepatic steatosis in humans and animals. Here, we demonstrate changes metabolites lipids explain underlying mechanism of ezetimibe steatosis. Methods We fed Otsuka Long-Evans Tokushima Fatty (OLETF) rats a high-fat diet (60 kcal % fat) with or vehicle (control) (10 mg kg -1 ) via stomach gavage for 12 weeks performed comprehensive metabolomic lipidomic profiling...