Metabolite profiling of red and white pitayas (Hylocereus polyrhizus Hylocereus undatus) was performed using gas chromatography-time-of-flight-mass spectrometry ultraperformance liquid chromatography-quadrupole-time-of-flight-mass with multivariate analysis. Different species parts (red peel, RP; WP; flesh, RF; WF) were clearly separated by partial least-squares discriminate Furthermore, betalain-related metabolites, such as betacyanins betaxanthins, or their precursors described on the...

Polycystic ovary syndrome (PCOS) is an endocrinal disorder that afflicts mainly women of childbearing age. The symptoms PCOS are irregular menstrual cycles, weight gain, subfertility and infertility. However, because the etiology unclear, management treatment methods for not well established. Recently, natural substances have been used therapy. Ecklonia cava (E. cava) a well-known substance attenuates effects inflammation, allergies cancer. In this study, we investigated E. extract in rats...

The objective of this study was to isolate multifunctional bacteriocin-producing strains; characterize the expressed bacteriocin for control Listeria monocytogenes and vancomycin-resistant Enterococcus; evaluate safety studied explore their antifungal activity.Two Pediococcus strains were isolated from silage samples obtained an organic farm in Belogradchik, Bulgaria. identified by 16S rRNA sequencing analysis characterized as bacteriocins producers. Strong antimicrobial activity detected...

Current understanding of heat shock response has been complicated by the fact that stress is inevitably accompanied changes in specific growth rates and stages. In this study, a chemostat culture was successfully performed to avoid physico-chemical biological accompany heatshock, which provided unique opportunity investigate full range cellular responses thermal stress, ranging from temporary adjustment phenotypic adaptation at multi-omics levels. Heat-responsive time-resolved transcriptome...

Background and Purpose Thiazolidinediones, acting as PPAR‐γ ligands, reduce hepatic steatosis in humans animals. However, the underlying mechanism of this action remains unclear. The purpose study was to investigate changes metabolites lipids response treatment with thiazolidinedione pioglitazone an animal model obese Type 2 diabetes. Experimental Approach Male Otsuka Long‐Evans Tokushima Fatty (OLETF) rats were orally administered either vehicle (control) or (30 mg·kg −1 ) fed a high‐fat...

We used ultra-performance-liquid-chromatography with quadrupole-time-of-flight mass spectrometry to study the changes in metabolites mixture of Cudrania tricuspidata, Lonicera caerulea, and soybean (CLM) during fermentation. Additionally, antiobesity effects CLM fermented-CLM (FCLM) were studied based on analysis plasma from high-fat diet (HFD)-fed mice. The levels cyanidin glycosides luteolin, quercetin, derived L. caerulea decreased, whereas luteolin quercetin increased Isoflavone...

To investigate the pathophysiologic characteristics of diabetic complications, we identified differences in plasma metabolites subjects with type 2 diabetes (T2DM) or without macular edema (DME) and a disease duration > 15 years. An cohort older T2DM patients prolonged was established, clinical information biospecimens were collected following guidelines National Biobank Korea. DME phenotypes by ophthalmologic specialists. For metabolomics studies, propensity matched case control samples...

Summary Chronic respiratory diseases are part of accumulating health problems partly due to worldwide increase in air pollution. By their antimicrobial and immunomodulatory properties, some probiotics constitute promising alternatives for the prevention treatment chronic diseases. We have isolated Bacillus strains from Korean fermented foods selected three potentially probiotic (two subtilis one amyloliquefaciens ) based on safety, efficacy, activity against airborne pathogens properties...

Background Ezetimibe, which lowers cholesterol by blocking the intestinal transporter Niemann-Pick C1 like 1, is reported to reduce hepatic steatosis in humans and animals. Here, we demonstrate changes metabolites lipids explain underlying mechanism of ezetimibe steatosis. Methods We fed Otsuka Long-Evans Tokushima Fatty (OLETF) rats a high-fat diet (60 kcal % fat) with or vehicle (control) (10 mg kg -1 ) via stomach gavage for 12 weeks performed comprehensive metabolomic lipidomic profiling...

Scope The esoteric anti‐obesity effects of hyacinth bean ( Dolichos lablab L) have largely remained unexplored. Herein, we investigated the mechanisms compared to milk thistle, a natural herb employed for ameliorating obesity‐related diseases, using high‐fat diet (HFD) fed mice towards unfolding perplexing mechanisms. Methods and results C57BL/6J were orally administered (25 mg/kg/day) thistle (100 9 weeks along with HFD. Intriguingly, number indexed through clinical parameters, suppression...

Berries have been used as valuable sources of polyphenols for human health; however, injudicious uses berries are widespread without regard to the specific metabolite constituent each berry. We classified 6 different edible (honeyberry, blueberry, mandarin melonberry, mulberry, chokeberry, and Korean black raspberry) based on their distributions in biosynthetic pathways by non-targeted profiling bioactive correlation analysis. Principal component analysis revealed a distinct clustering...

Phytonutrients and vitamin mineral supplementation have been reported to provide increased antioxidant capacity in humans; however, there is still controversy. In the current clinical trial, we examined DNA protection of a plant-based, multi-vitamin/mineral, phytonutrient (PMP) healthy adults who were habitually low consumption fruits vegetables. This study was an eight-week, double-blind, randomized, parallel-arm, placebo-controlled trial. PMP for eight weeks reduced reactive oxygen species...

Temporal geo-climatic variations are presumably vital determinants of phenotypic traits and quality characteristics berries manifested through reconfigured metabolomes. We performed an untargeted mass spectrometry (MS)-based metabolomic analysis blueberry (Vaccinium spp.) chokeberry (Aronia melanocarpa) sample extracts harvested from different regions in Korea. The multivariate statistical indicated distinct metabolite compositions berry groups based on species regions. amino acids levels...

A high-fiber diet is widely recognized for its positive effects on the gut microbiome. However, specific impact of a microbiome and bowel habits patients with colon cancer remains poorly understood. In this study, we aimed to assess modified microbiota-accessible carbohydrate (mMAC) microbiota composition clinical symptoms in who underwent surgical resection. To achieve this, enrolled 40 two groups: those received adjuvant chemotherapy did not. Fecal samples were collected before after...

Atopic dermatitis (AD) is a chronic inflammatory skin condition influenced by various factors, such as the microbiome and metabolome. However, specific contributions of these factors to scalp involvement in AD still need be explored. In this study, we aimed assess associations between metabolome patients with healthy controls (HCs).

In order to achieve premium quality with crop production, techniques involving the adjustment of nutrient supply and/or supplemental lighting specific light have been applied. To examine effects low mineral and lighting, we performed non-targeted metabolite profiling leaves stems medicinal herb

Abstract Apoptosis has been suggested as the first step in process of conversion muscle into meat. While a potential role apoptosis postmortem proteolysis proposed, underlying mechanisms by which metabolome changes muscles would influence apoptotic and proteolytic process, leading to meat quality variation, not determined. Here, attributes metabolomics profiling longissimus dorsi (LD) psoas major (PM) pigs from two different production cycles (July–Jan vs. Apr–Sep) were evaluated. PM showed...

The characterization and temporal changes of intracellular extracellular metabolome Lactiplantibacillus plantarum under different growth times were studied using multi-platform metabolomics. A approach consisting both non-targeted profiling targeted quantification has identified a total 168 metabolites among which, 41 110 in the intra- metabolomes determined to be significantly different. We constructed an expanded metabolic pathway map that combines amino acid, fatty carbohydrate, purine,...

High salt accumulation, resulting from the rampant use of chemical fertilizers in greenhouse cultivation, has deleterious effects on plant growth and crop yield. Herein, we delineated magnesium (Mg) oversupply Perilla frutescens leaves, a traditional edible medicinal herb used East-Asian countries. Mg resulted significantly higher chlorophyll content coupled with lower antioxidant activities growth, suggesting direct effect subtle metabolomes. The relative abundance bioactive phytochemicals,...

Plants are an important and inexhaustible source of bioactive molecules in food, medicine, agriculture, industry. In this study, we performed systematic liquid chromatography–mass spectrometry (LC-MS)-based metabolic profiling coupled with antioxidant assays for indigenous plant family extracts. Partial least-squares discriminant analysis LC-MS datasets the extracts 34 species belonging to families Aceraceae, Asteraceae, Rosaceae showed that these were clustered according their respective...

Abstract Background: Previous studies have indicated differing patterns of the microbiome in colorectal cancer (CRC) patients compared to healthy individuals. Yet, changes microbial features CRC before and after surgery remain underexplored. This study aims investigate impact surgical intervention on gut diversity abundance at patient level. Methods: hospital-based analyzed data from 36 who underwent Department Surgery, Seoul National University Hospital between January 2020 May 2022. Fecal...

Abstract Background Inflammatory Bowel Disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC), presents diagnostic challenges owing to overlapping clinical presentations. This study aimed delineate specific serum metabolomic biomarkers that differentiate IBD patients from healthy controls further discriminate between CD UC. Methods We enrolled a total of 346 participants, including 134 with CD, 124 UC, 88 normal (NC). Serum samples their metadata were systematically...