A5033944587- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Mycology
- Antibiotics Pharmacokinetics and Efficacy
- Infectious Diseases and Tuberculosis
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- Parallel Computing and Optimization Techniques
- Aquaculture disease management and microbiota
- Fish biology, ecology, and behavior
- Analytical Chemistry and Chromatography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ginseng Biological Effects and Applications
- Clinical Laboratory Practices and Quality Control
- Fault Detection and Control Systems
- Calpain Protease Function and Regulation
Fundação Oswaldo Cruz
2003-2024
Escola Nacional de Saúde Pública
2012-2020
In this article, the first isolation of Mycobacterium kyorinense specimens in Brazil is described. M. a recently identified species, with few strains reported only Japan. The Brazilian isolates were initially as celatum by PCR restriction enzyme pattern analysis (PRA) hsp65. However, biochemical tests indicated same profile and distinguished them from branderi. sequencing hsp65, rpoB, 16S rRNA genes allowed accurate identification kyorinense.
Three isolates of a slow-growing, non-chromogenic mycobacterium were grown from three sputum samples patient the north-eastern Ceará state in Brazil. Identification at species level could not be obtained with PCR restriction analysis hsp65 gene. In order to characterize we carried out phenotypic and genotypic tests. We sequenced nearly complete 16S rRNA gene partial sequences (encoding hypervariable region 65 kDa heat-shock protein) rpoB beta-subunit RNA polymerase) genes. The turned...
The present study aimed to determine the genetic diversity of isolates Mycobacterium tuberculosis (Mtb) from presumed drug resistant patients several states Brazil. had been submitted conventional susceptibility testing for first- and second-line drugs. Multidrug-resistant (MDR-TB) (54.8%) was most frequent phenotypic resistance profile, in addition an important high frequency pre-extensively (p-XDR-TB) (9.2%). Using whole-genome sequencing (WGS), we characterized 298 Mtb Besides analysis...
In order to evaluate the Organon Teknika MB/BacT system used for testing indirect susceptibility alternative drugs ofloxacin (OFLO), amikacin (AMI), and rifabutin (RIF), usual of standard treatment regimes such as rifampin (RMP), isoniazid (INH), pyrazinamide (PZA), streptomycin (SM), ethambutol (EMB), ethionamide (ETH), cultures clinical specimens from 117 patients with pulmonary tuberculosis under multidrug-resistant investigation, admitted sequentially examination 2001 2002, were studied....
The present study aimed to determine the genetic diversity of isolates Mycobacterium tuberculosis (Mtb) from presumed drug-resistant patients several states Brazil. had been submitted conventional drug susceptibility testing for first- and second-line drugs. Multidrug-resistant (MDR-TB) (54.8%) was most frequent phenotypic resistance profile, in addition an important high frequency pre-extensive (p-XDR-TB) (9.2%). Using whole-genome sequencing (WGS), we characterized 298 Mtb Besides analysis...
In order to evaluate the direct-method test of sensitivity drugs used in principal tuberculosis treatment regimes, Organon Teknika MB/BacT system, we tested 50 sputum samples positive microscopy taken from patients with pulmonary and clinical indications for an antibiogram, admitted sequentially examination during routine reference laboratory. The material was treated v/v 23% trisodium phosphate solution, incubated 24 h at 35 degrees C, neutralized 20% monosodium solution. then centrifuged...
(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance first-line TB treatment drugs. present study aimed at evaluating its performance in multicenter study. (2) Methods: To determine accuracy, the proportion methods Lowenstein Jensen medium or BACTECTMMGITTM960 system was as gold standard. (3) Results: revealed that respective accuracies of with 190 M. clinical isolates, using standard, were 93.9%...
Os maiores valores de concordância para INH e RMP assim como menores SM foram similarmente observados por Laszlo et al.,1997. A pobre observada o EMB merece especial atencao um maior numero amostras resistentes melhor avaliacao. insumos (meio Lowenstein-Jensen , sais das drogas amostras-tipo) sao padronizados fornecidos pelo laboratorio referencia todos os LACENS. qualidade dos utilizados a capacitacao tecnica do profissional essenciais obtermos resultados confiaveis em exames laboratoriais.