A5021730505- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Alzheimer's disease research and treatments
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Trace Elements in Health
- Glycosylation and Glycoproteins Research
- Legume Nitrogen Fixing Symbiosis
- Drug Transport and Resistance Mechanisms
- Amino Acid Enzymes and Metabolism
- Molecular Sensors and Ion Detection
- Cancer Research and Treatments
- Pediatric Hepatobiliary Diseases and Treatments
- Plant Gene Expression Analysis
- Agronomic Practices and Intercropping Systems
- Folate and B Vitamins Research
- RNA Research and Splicing
- Lipid Membrane Structure and Behavior
- Aluminum toxicity and tolerance in plants and animals
- Cancer, Lipids, and Metabolism
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Enzyme Production and Characterization
University of Science and Technology of China
2018-2025
Hefei National Center for Physical Sciences at Nanoscale
2018-2025
Nanjing Forestry University
2024
Center for Excellence in Molecular Cell Science
2018-2023
Harbin Institute of Technology
2023
Nanjing Agricultural University
2023
Guangzhou Medical University
2023
Second Affiliated Hospital of Guangzhou Medical University
2023
Chinese Academy of Sciences
2018-2021
Center for Life Sciences
2014-2016
A hallmark of Alzheimer's disease (AD) is the aggregation β-amyloid peptides (Aβ) into amyloid plaques in patient brain. Cleavage precursor protein (APP) by intramembrane protease γ-secretase produces Aβ varying lengths, which longer such as Aβ42 are thought to be more harmful. Increased ratios Aβs over shorter ones, exemplified ratio Aβ40, may lead formation and consequent development AD. In this study, we analyzed 138 reported mutations human presenilin-1 (PS1) individually reconstituting...
Polar auxin transport is unique to plants and coordinates their growth development1,2. The PIN-FORMED (PIN) transporters exhibit highly asymmetrical localizations at the plasma membrane drive polar transport3,4; however, structures mechanisms remain largely unknown. Here, we report three inward-facing conformation of Arabidopsis thaliana PIN1: apo state, bound natural indole-3-acetic acid (IAA), in complex with inhibitor N-1-naphthylphthalamic (NPA). transmembrane domain PIN1 shares a...
Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. synthesized in the cell interior but bind receptors at surface, necessitating a yet be identified export mechanism. Here, we show member of ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as brassinosteroid exporter. We present its structure both substrate-unbound brassinosteroid-bound states. Bioactive brassinosteroids potent...
Virulent enteric pathogens such as Escherichia coli strain O157:H7 rely on acid-resistance (AR) systems to survive the acidic environment in stomach. A major component of AR is an arginine-dependent arginine:agmatine antiporter that expels intracellular protons. Here, we report crystal structure AdiC, from E. and a member amino acid/polyamine/organocation (APC) superfamily transporters at 3.6 resolution. The overall fold similar several Na+-coupled symporters. AdiC contains 12 transmembrane...
In plants, hyperosmolality stimuli triggers opening of the osmosensitive channels, leading to a rapid downstream signaling cascade initiated by cytosolic calcium concentration elevation. Members OSCA family in Arabidopsis thaliana, identified as hyperosmolality-gated calcium-permeable have been suggested play key role during initial phase hyperosmotic stress response. Here, we report atomic structure OSCA1.2 determined single-particle cryo-electron microscopy. It contains 11 transmembrane...
Significance The crystallographic model of the Major Facilitator Superfamily (MFS) member, d -xylose permease XylE from Escherichia coli , a homologue human -glucose transporters, GLUTs (SLC2), provides structural framework for identification and physical localization crucial residues in transporters with medical relevance (i.e. GLUTs). mechanism substrate specificity prokaryotic sugar are discussed by using homology modeling, molecular docking, experimentation. Substrate-specificity...
Significance Unlike other single-component intramembrane proteases such as rhomboid and S2P, γ-secretase contains four components: presenilin, Pen-2, Aph-1, nicastrin. Previous electron cryomicroscopy (cryo-EM) analysis of human in amphipols revealed its overall architecture 19 distinct transmembrane segments (TMs). However, the lack side-chain density TMs, together with disordered inter-TM loops, disallowed TM assignment. Our current cryo-EM structure at 4.32-Å resolution allows specific...
Abstract Abscisic acid (ABA) is a phytohormone essential to the regulation of numerous aspects plant growth and development. The cellular level ABA critical its signalling determined by rate biosynthesis, catabolism rates transport. ABCG25 in Arabidopsis thaliana has been identified be an exporter play roles regulating stomatal closure seed germination. However, transport mechanism remains unknown. Here we report structures under different states using cryo-electron microscopy single...
Cellular zinc (Zn
In mitosis, accurate chromosome segregation depends on kinetochores that connect centromeric chromatin to spindle microtubules. The centromeres of budding yeast, which are relatively simple, connected individual microtubules via a kinetochore constitutive centromere associated network (CCAN). However, the complex human chromosomes comprise millions DNA base pairs and attach multiple Here, by use cryo-electron microscopy functional analyses, we reveal molecular basis how CCAN interacts with...
Significance γ-Secretase is a four-component intramembrane protease associated with the onset of Alzheimer's disease. Nicastrin putative substrate-recruiting component γ-secretase complex, but no atomic-resolution structure had been identified on or any its four components. Here we report first crystal eukaryotic nicastrin which shares significant sequence homology human nicastrin. This reveals fine details and allows modeling Analysis structural yields working model showing how might...
The wall teichoic acid (WTA) is a major cell component of Gram-positive bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), common cause fatal clinical infections in humans. Thus, the indispensable ABC transporter TarGH, which flips WTA from cytoplasm to extracellular space, becomes promising target anti-MRSA drugs. Here, we report 3.9-Å cryo-electron microscopy (cryo-EM) structure 50% sequence-identical homolog TarGH Alicyclobacillus herbarius at an ATP-free and...
The small conductance mechanosensitive ion channel (MscS)-like (MSL) proteins in plants are evolutionarily conserved homologs of the bacterial channels. As sole member Arabidopsis MSL family localized mitochondrial inner membrane, MSL1 is essential to maintain normal membrane potential mitochondria. Here, we report a cryoelectron microscopy (cryo-EM) structure thaliana (AtMSL1) at 3.3 Å. overall architecture AtMSL1 similar MscS. However, transmembrane domain larger. Structural differences...
The transient receptor potential channel subfamily A member 1 (TRPA1) ion is an evolutionary conserved polymodal sensor responding to noxious temperature or chemical stimuli. Notably, the thermosensitivity of TRPA1 varies among different species even isoforms in same species. However, underlying molecular basis its thermo-gating remains largely unknown. Here, we determine structures a heat-sensitive isoform Drosophila melanogaster two distinct conformations with cryo-samples prepared at 8...
Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs the apoplast is essential BR signaling initiation, which requires binding a molecule extracellular domains plasma membrane-localized receptor complex. We have previously shown that Arabidopsis thaliana ATP-binding cassette (ABC) transporter ABCB19 functions as exporter and, together with its close homolog ABCB1, positively...