A5101399654- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- RNA Research and Splicing
- Plant Molecular Biology Research
- Venomous Animal Envenomation and Studies
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Protein Structure and Dynamics
- Plant Reproductive Biology
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Porphyrin Metabolism and Disorders
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Platelet Disorders and Treatments
- Galectins and Cancer Biology
- Neurological diseases and metabolism
- Endoplasmic Reticulum Stress and Disease
University of Science and Technology of China
2015-2025
Hefei National Center for Physical Sciences at Nanoscale
2008-2024
Center for Life Sciences
2022-2024
Chinese Academy of Sciences
2007-2016
Hefei Institutes of Physical Science
2008-2016
Polar auxin transport is unique to plants and coordinates their growth development1,2. The PIN-FORMED (PIN) transporters exhibit highly asymmetrical localizations at the plasma membrane drive polar transport3,4; however, structures mechanisms remain largely unknown. Here, we report three inward-facing conformation of Arabidopsis thaliana PIN1: apo state, bound natural indole-3-acetic acid (IAA), in complex with inhibitor N-1-naphthylphthalamic (NPA). transmembrane domain PIN1 shares a...
Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. synthesized in the cell interior but bind receptors at surface, necessitating a yet be identified export mechanism. Here, we show member of ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as brassinosteroid exporter. We present its structure both substrate-unbound brassinosteroid-bound states. Bioactive brassinosteroids potent...
Abstract Abscisic acid (ABA) is a phytohormone essential to the regulation of numerous aspects plant growth and development. The cellular level ABA critical its signalling determined by rate biosynthesis, catabolism rates transport. ABCG25 in Arabidopsis thaliana has been identified be an exporter play roles regulating stomatal closure seed germination. However, transport mechanism remains unknown. Here we report structures under different states using cryo-electron microscopy single...
Cellular zinc (Zn
Abstract The interaction of platelet GPIbα with von Willebrand factor (VWF) is essential to initiate adhesion and thrombosis, particularly under high shear stress conditions. However, no drug targeting has been developed for clinical practice. Here we characterized anfibatide, a antagonist purified from snake ( Deinagkistrodon acutus ) venom, evaluated its by surface plasmon resonance in silico modeling. We demonstrated that anfibatide interferds both VWF thrombin binding, inhibited...
The transient receptor potential channel subfamily A member 1 (TRPA1) ion is an evolutionary conserved polymodal sensor responding to noxious temperature or chemical stimuli. Notably, the thermosensitivity of TRPA1 varies among different species even isoforms in same species. However, underlying molecular basis its thermo-gating remains largely unknown. Here, we determine structures a heat-sensitive isoform Drosophila melanogaster two distinct conformations with cryo-samples prepared at 8...
Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs the apoplast is essential BR signaling initiation, which requires binding a molecule extracellular domains plasma membrane-localized receptor complex. We have previously shown that Arabidopsis thaliana ATP-binding cassette (ABC) transporter ABCB19 functions as exporter and, together with its close homolog ABCB1, positively...
Abstract Stomata play a critical role in the regulation of gas exchange and photosynthesis plants. Stomatal closure participates multiple stress responses, is regulated by complex network including abscisic acid (ABA) signaling ion-flux-induced turgor changes. The slow-type anion channel SLAC1 has been identified to be central controller stomatal phosphoactivated several kinases. Here, we report structure Arabidopsis thaliana ( At SLAC1) an inactivated, closed state. cytosolic amino...
The phytohormone auxin plays central roles in many growth and developmental processes plants. Development of chemical tools targeting the pathway is useful for both plant biology agriculture. Here we reveal that naproxen, a synthetic compound with anti-inflammatory activity humans, acts as an transport inhibitor PIN-FORMED (PIN) transporters Physiological experiments indicate exogenous naproxen treatment affects pleiotropic auxin-regulated processes. Additional cellular biochemical evidence...
Cytokinins are adenine derivatives serving as phytohormones, which essential regulators of plant growth, development, and response to environmental factors. The transport process cytokinins determines their spatial distributions is critical functions. AZA-GUANINE RESISTANT (AZG) family member AZG2 in Arabidopsis thaliana has been identified a cytokinin purine transporter. Here, we characterize the binding towards natural cytokinin, trans-zeatin (tZ), well adenine. structures determined both...
Fungal laccases are oxidoreductases that belong to the multinuclear copper-containing oxidases. They able oxidize a wide range of substrates, preferably phenolic compounds, which makes them suitable for employment in bioremediation soil and water as well other biotechnological applications. Here, structural analysis natural laccase B (LacB) from Trametes sp. AH28-2 is presented. This structure provides opportunity study post-translational modifications enzyme. The overall fold shows high...
Agkisacucetin is a snake C-type lectin isolated from the venom of Agkistrodon acutus (A. acutus). It binds specifically to platelet glycoprotein (GP) Ib and prevents von Willebrand factor (VWF) accessing it. We determined crystal structure agkisacucetin 1.9Å resolution. The has an (αβ) fold similar another GPIb-binding protein, flavocetin-A, but lacks C-terminal cysteine in β-subunit, does not form (βα)(4) tetramers, cluster GPIbs, like flavocetin-A.
In most eukaryotes, poly(A) tails are attached to the 3′-ends of mRNAs. They influence almost all aspects mRNA metabolism: transport from nuclear cytoplasm, translation, and turnover. To mediate its biological functions, covered in sequence-specific manner by two types conserved binding proteins, PABPC cytoplasm PABPN1 nucleus. (Pab1p Saccharomyces cerevisiae1) is present eukaryotes. It plays important roles initiation translation2 decay.3 contains four copies RRMs [the RNA recognition...
Lectins are a group of proteins that recognize carbohydrates covalently linked to and lipids on the cell surface within extracellular matrix have diverse physiological functions1-3 including growth regulation,4-6 adhesion,7, 8 pre-mRNA splicing,9, 10 migration,11, 12 apoptosis,13-15 immune responses,16 pathogen recognition.17 The galectins family animal lectins defined by their shared conserved carbohydrate recognition domain (CRD) about 130 amino acids affinity for β-galactoside sugars.18,...
The Nit (nitrilase-like) protein subfamily constitutes branch 10 of the nitrilase superfamily. proteins are widely distributed in nature. Mammals possess two members subfamily, namely Nit1 and Nit2. Based on sequence similarity, yeast Nit2 (yNit2) is a homologue mouse Nit1, tumour-suppressor whose substrate specificity not yet known. Previous studies have shown that mammalian (also putative tumour suppressor) identical to ω-amidase, an enzyme catalyzes hydrolysis α-ketoglutaramate (α-KGM)...