A5032009998- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Memory and Neural Mechanisms
- Cancer, Stress, Anesthesia, and Immune Response
- Multiple Sclerosis Research Studies
- Sphingolipid Metabolism and Signaling
- Cellular transport and secretion
- Stress Responses and Cortisol
- Neonatal and fetal brain pathology
- Olfactory and Sensory Function Studies
- Wnt/β-catenin signaling in development and cancer
- Parkinson's Disease Mechanisms and Treatments
- Autism Spectrum Disorder Research
- Blood Coagulation and Thrombosis Mechanisms
- Adenosine and Purinergic Signaling
- Peptidase Inhibition and Analysis
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Neurotransmitter Receptor Influence on Behavior
- Alzheimer's disease research and treatments
Istituto Neurologico Mediterraneo
2021-2025
Sapienza University of Rome
2012-2024
Oasi Maria SS
2014-2015
The identification of mechanisms that mediate stress-induced hippocampal damage may shed new light into the pathophysiology depressive disorders and provide targets for therapeutic intervention. We focused on secreted glycoprotein Dickkopf-1 (Dkk-1), an inhibitor canonical Wnt pathway, involved in neurodegeneration. Mice exposed to mild restraint stress showed increased levels Dkk-1 reduced expression β-catenin, intracellular protein positively regulated by signalling pathway. In...
Metabotropic glutamate (mGlu) receptors are candidate drug targets for therapeutic intervention in Parkinson's disease (PD). Here we focused on mGlu3, a receptor subtype involved synaptic regulation and neuroinflammation. mGlu3−/− mice showed an enhanced nigro-striatal damage microglial activation response to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Expression of genes encoding anti-inflammatory proteins neuroprotective factors was reduced the striatum MPTP-treated mice. We also...
Abstract Recent findings indicate that fingolimod, the first oral drug approved for treatment of multiple sclerosis ( MS ), acts as a direct inhibitor histone deacetylases HDAC s) and enhances production brain‐derived neurotrophic factor BDNF ) in CNS . Both mechanisms are relevant to pathophysiology major depression. We examined antidepressant activity fingolimod mice subjected chronic unpredictable stress CUS model reactive depression endowed with face pharmacological validity. Chronic (3...
Abstract Background Perinatal inflammation is a key factor of brain vulnerability in neonates born preterm or with intra-uterine growth restriction (IUGR), two leading conditions associated injury and responsible for neurocognitive behavioral disorders. Systemic recognized to activate microglia, known be the critical modulators vulnerability. Although some evidence supports role metabotropic glutamate receptor 3 (mGlu3 receptor) modulation neuroinflammation, its functions are still unknown...
Cigarette smoking is a recognized risk factor for cardiovascular diseases and has been implicated in the pathogenesis of atherosclerosis thrombotic events. In athero-thrombotic diseases, extracellular adenine nucleotides play an important role by triggering range effects such as recruitment activation platelets, endothelial cell vasoconstriction. NTPDase, plasma membrane-bound enzyme, most relevant enzyme involved hydrolysis tri- di-phosphate to adenosine monophosphate, which further...
The α2 δ subunit of voltage-sensitive calcium channels (VSCCs) is the molecular target pregabalin and gabapentin, two drugs marked for treatment focal epilepsy, neuropathic pain, anxiety disorders. Expression up-regulated in dorsal horns spinal cord models suggesting that plastic changes are associated with pathological states. Here, we examined expression δ-1 amygdala, hippocampus, frontal cortex trimethyltiazoline (TMT) mouse model innate anxiety. TMT a volatile molecule present feces...
mGlu5 receptor-mediated polyphosphoinositide (PI) hydrolysis is classically measured by determining the amount of radioactivity incorporated in inositolmonophosphate (InsP) after labeling membrane phospholipids with radioactive inositol. Although this method historically linked to study mGlu receptors, it inappropriate for assessment receptor signaling vivo. Using a new ELISA kit we showed that systemic treatment selective positive allosteric modulator (PAM) receptors VU0360172 enhanced InsP...
We studied group-I metabotropic glutamate (mGlu) receptors in Pahenu2 (ENU2) mice, which mimic the genetics and neurobiology of human phenylketonuria (PKU), a metabolic disorder characterized, if untreated, by autism, intellectual disability (ID). Male ENU2 mice showed increased mGlu5 receptor protein levels hippocampus corpus striatum (but not prefrontal cortex) whereas transcript was unchanged. No changes mGlu1 mRNA were found any three brain regions mice. extended analysis to Homer...
Using an in vivo method for the assessment of polyphosphoinositide (PI) hydrolysis, we examine whether spatial learning and memory extinction cause changes mGlu5 metabotropic glutamate receptor signaling hippocampus prefrontal cortex. We use following five groups mice: (i) naive mice; (ii) control mice exposed to same environment as learner (iii) leaner mice, trained four days a water maze; (iv) which was induced by six trials without platform; (v) that spontaneously lost memory. The...
The epigenetic agents, L-acetylcarnitine (LAC) and L-methylfolate (MF) are putative candidates as add-on drugs in depression. We evaluated the effect of a combined treatment with LAC MF two different paradigms chronic stress mice human inducible pluripotent stem cells (hiPSCs) differentiated into dopaminergic neurons. Two groups were exposed to unpredictable (CUS) for 28 days or restraint (CRS) 21 day, (30 100 mg/kg) and/or (0.75 3 administered i.p. once day 14 days, starting from last week...
Background: Glioblastoma (GBM) represents an aggressive and common tumor of the central nervous system. The prognosis GBM is poor, despite a refined genetic molecular characterization, pharmacological treatment largely suboptimal. Objective: Contribute to defining therapeutic line in targeting mGlu3 receptor with principles precision medicine. Methods: Here, we performed computational analysis focused on expression type 3 5 metabotropic glutamate subtypes (mGlu3 mGlu5, respectively) high-...
Abstract Background: Perinatal inflammation is a key factor of brain vulnerability in neonates born preterm or with intra-uterine growth restriction (IUGR), two leading conditions associated injury and responsible for neurocognitive behavioral disorders. Systemic recognized to activate microglia, known be the critical modulators vulnerability. Although some evidence support role metabotropic glutamate receptor 3 (mGlu3 receptor) modulation neuroinflammation, its functions are still unknown...