A5072933126- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Muscle Physiology and Disorders
- Drug Transport and Resistance Mechanisms
- Cytokine Signaling Pathways and Interactions
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Acute Lymphoblastic Leukemia research
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- HER2/EGFR in Cancer Research
- Extracellular vesicles in disease
- Metastasis and carcinoma case studies
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Cancer-related molecular mechanisms research
- NF-κB Signaling Pathways
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Heat shock proteins research
- Histone Deacetylase Inhibitors Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Natural Compounds in Disease Treatment
Instituto Nacional do Câncer
2012-2025
Universität Ulm
2017
Faculdade de Ciências Médicas da Santa Casa de São Paulo
2014
Universidade Federal do Rio de Janeiro
2013-2014
Celula (United States)
2014
University Hospital Ulm
2014
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
2013
Centro de Estudos e Pesquisa em Saúde Coletiva
2012
The metastatic process in breast cancer is related to the expression of epithelial-to-mesenchymal transition transcription factors (EMT-TFs) SNAIL, SLUG, SIP1 and TWIST1. EMT-TFs nuclear factor-κB (NF-κB) activation have been associated with aggressiveness potential carcinomas. Here, we sought examine role NF-κB aggressive properties regulation human cells. Blocking NF-κB/p65 activity by reducing its transcript protein levels (through siRNA-strategy dehydroxymethylepoxyquinomicin [DHMEQ]...
Abstract Background The advanced phases of chronic myeloid leukemia (CML) are known to be more resistant therapy. This resistance has been associated with the overexpression ABCB1 , which gives rise multidrug (MDR) phenomenon. MDR is characterized by nonrelated drugs, and P-glycoprotein (encoded ) implicated as major cause its emergence. Wnt signaling demonstrated important in several aspects CML. Recently, was linked regulation through canonical pathway, mediated β-catenin, other types...
The therapeutic potential of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) in kidney injury has been largely reported. However, new approaches are necessary to optimize the efficacy treatment renal diseases. MSCs physiologically under a low O2 partial pressure (pO2), and culturing adipose-derived (ADMSCs) hypoxia alters their secretory paracrine properties. aim this study was evaluate whether preconditioning ADMSCs properties secreted EVs improve recovery after...
Background/Objectives: Despite its heterogeneity and diagnostic challenges, acute myeloid leukemia (AML) originates from stem cell transformation alterations in the hematopoietic niche (HN) could be related to leukemic transformation. Therefore, aim of this study was evaluate protein profile HN AML patients compare it with healthy donors (HDs). Methods: A proteomic analysis conducted identify differentially expressed (DE) proteins BM plasma HD. In silico performed biological processes...
To better characterize the cellular pathways involved in breast cancer molecular subtypes, we performed a proteomic study using label-free LC-MS strategy for determining profile of Luminal A, Luminal-HER2, HER2-positive, and triple-negative (TN) tumors compared with healthy mammary tissue. This comparison aimed to identify aberrant processes specific each subtype might help refine our understanding regarding biology. Our results address important features (both commonly shared) that explain...
Among the many challenges in cancer diagnosis is early distinction between metastatic and a secondary tumor. This difficulty stems from lack of markers that offer high sensitivity specificity can be easily applied routine laboratory work. An example this challenge distinguishing gastric metastases originating breast primary Hepatocyte nuclear factor 4 alpha (HNF4A) has been suggested as potential marker these cases. The aim study was to analyze expression HNF4A, estrogen receptor (ER),...
One of the potential mechanisms imatinib mesylate (IM) resistance in chronic myeloid leukemia (CML) is increased level P-glycoprotein (Pgp). Pgp an efflux pump capable activating multidrug (MDR) phenotype. The gene encoding (ABCB1) has several binding sites its promoter region, along with CpG islands and GC boxes, involved epigenetic control. In previous work, we performed a proteomic study to identify proteins IM cross-resistance acute leukemia. Among these proteins, identified LRPPRC as...
Abstract Using chip array assays, we identified differentially expressed genes via a comparison between luminal A breast cancer subtype and normal mammary ductal cells from healthy donors. In silico analysis confirmed by western blot immunohistochemistry revealed that C-JUN C-FOS transcription factors are activated in patients as potential upstream regulators of these genes. chip-on-chip assay, targets. Among genes, the NRIP1 gene was to be targeted C-FOS. This after identification...
Although chronic myeloid leukemia (CML) treatment has improved since the introduction of imatinib mesylate (IM), cases resistance have been reported. This associated with emergence multidrug (MDR) phenotype, as a BCR-ABL independent mechanism. The classic pathway studied in MDR promotion is ATP-binding cassette (ABC) family transporters expression, but other mechanisms that drive drug are largely unknown. To better understand IM therapy relapse due to rise MDR, we compared proteomic profiles...
Mesenchymal stromal cells (hMSCs) are key components of the bone marrow microenvironment (BMM). A molecular signature in hMSCs from Acute myeloid leukemia patients (hMSC-AML) has been proposed where BMP4 is decreased and could be regulated by WNT signaling pathway. Therefore, aim this work was to verify whether pathway can regulate gene hMSCs. The results showed differentially expressed genes canonical between hMSC-AML healthy donors a real-time quantitative assay corroborated with these...
Turner syndrome (TS) is characterized by the presence of one full X chromosome and total or partial deletion second sex chromosome. Diagnosis TS often delayed, resulting in inappropriate treatment. Early diagnosis using a neonatal screening test may improve preventive measures treatment, thus improving patient quality life. The goal this study was to standardize algorithm. Two genes (ARSE MAGEH1) 1 normalizing gene (HBB) were used detect We screened 996 newborns whose peripheral blood...
Abstract Mesenchymal stromal cells (MSCs) were first used as a source for cell therapy in 1995; however, despite their versatility and unambiguous demonstration of efficacy safety preclinical/phase I studies, the positive effect MSCs human phase III studies did not resemble success obtained mouse models disease. This dissonance highlights need to more thoroughly study immunobiology make better use these cells. Thus, we aimed by using chip array analysis method general screening obtain global...
Abstract A wide variety of cellular processes and signaling events are regulated by the proteolytic enzyme γ‐secretase. Notch‐1 is one substrates γ‐secretase its role in regulation muscle differentiation has been well described. Importantly, besides Notch‐1, a number proteins have identified to undergo proteolysis To date, specific during embryonic skeletal not studied. Therefore, we address this question through analysis vitro grown chick myogenic cells formation multinucleated myotubes....
Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including master regulator Twist1. However, its role beyond EMT remains unclear. Our study aimed investigate Twist1, EMT, BC. In patients, we observed overexpression TWIST1 HER2+ group. silencing cells resulted upregulation 138...
Signal transducer and activator of transcription 3 (STAT3) is an important transcriptional factor frequently associated with the proliferation survival a large number distinct cancer types. However, signaling pathways mechanisms that regulate STAT3 activation remain to be elucidated. In this study we took advantage existing cellular models for chronic myeloid leukemia resistance, western blot, in vitro signaling, real time PCR, flow cytometry approaches cell cycle apoptosis evaluation siRNA...
Mutations of the nucleophosmin-1 (NPM1) gene in cytogenetically normal (CN) acute myeloid leukemia (AML) identify a group patients with more favorable prognosis. NPM1 encodes three main alternatively spliced isoforms R1(B23.1), R2(B23.2), and R3(B23.3). The expression splice variants R1, R2 R3 were higher AML compared to cells healthy volunteers (HVs), although RNA-seq analysis revealed enhanced also less differentiated HVs as well cells. variant R2, which lacks exons 11 12 coding for...
Lysosomes and acidic compartments are involved in breaking down of macromolecules, membrane recycling, regulation signaling pathways. Here, we analyzed the role during muscle differentiation involvement Wnt/beta-catenin pathway lysosomal function myogenesis. Acridine orange was used to localize quantify cellular primary cultures embryonic cells from Gallus gallus . Our results show an increase compartment size area, as well changes their positioning initial steps The inhibition by either...
Classical Hodgkin lymphoma (cHL) cells overexpress heat-shock protein 90 (HSP90), an important intracellular signaling hub regulating cell survival, which is emerging as a promising therapeutic target. Here, we report the antitumor effect of celastrol, anti-inflammatory compound and recognized HSP90 inhibitor, in Reed–Sternberg lines. Two disparate responses were recorded. In KM-H2 cells, celastrol inhibited proliferation, induced G0/G1 arrest, triggered apoptosis through activation...
CAPN3 is a muscle-specific and an intrinsically disordered protein. Thus, as scaffolding protein could play role during early stages of myogenesis. To test this hypothesis, we studied the distribution function myogenesis using embryonic chick muscle cells grown in vitro. Super-resolution microscopy showed (i) amorphous patches myoblasts, (ii) region near nuclei myotubes; (iii) adhesion plaques myotubes, (iv) stress fiber-like structures (v) filaments fibroblasts. Downregulation induced...