A5020461673- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Radiopharmaceutical Chemistry and Applications
- Polysaccharides and Plant Cell Walls
- Animal Genetics and Reproduction
- Immune responses and vaccinations
- T-cell and Retrovirus Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- HER2/EGFR in Cancer Research
- Polysaccharides Composition and Applications
- Protein purification and stability
- Cell Adhesion Molecules Research
- Neuroendocrine Tumor Research Advances
- Pancreatic and Hepatic Oncology Research
- Signaling Pathways in Disease
- Developmental Biology and Gene Regulation
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
- Pancreatitis Pathology and Treatment
- Cancer Immunotherapy and Biomarkers
- Liver Disease Diagnosis and Treatment
- Food composition and properties
- interferon and immune responses
Fujita Health University
2012-2023
Fujita Health University Hospital
2013-2020
University of Hawaiʻi at Mānoa
2015
Ecogenomics (Japan)
2014
Nagoya University
1999-2007
Gene Therapy Laboratory
2001
Conventional vaccines to combat COVID-19 through different approaches are at various stages of development. The complexity such as the potential mutations virus leading antigenic drift and uncertainty on duration immunity induced by vaccine have hampered efforts control pandemic. Thus, we suggest an alternative interim treatment strategy based biological response modifier glucans Aureobasidium pullulans AFO-202-derived β-glucan, which has been reported induce trained immunity, akin that...
Although several murine mAbs that have been humanized became useful therapeutic agents against a few malignancies, Abs are not yet available for the majority of human cancers because our lack knowledge which antigens (Ags) can become targets. In present study we established procedure comprehensive identification such Ags through extensive isolation may therapeutic. Using phage-display Ab library isolated large number bind to surface tumor cells. They were individually screened by...
The continued discovery of therapeutic antibodies, which address unmet medical needs, requires the tractable antibody targets. Multiple protein-level target approaches are available and these can be used in combination to extensively survey relevant cell membranomes. In this study, MDA-MB-231 line was selected for membranome as it is a 'triple negative' breast cancer line, represents subtype that aggressive has few treatment options.The carcinoma explore three approaches, were parallel...
<h3>Background</h3> Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are highly prevalent conditions characterized by inflammation fibrosis of the liver, which can progress to cirrhosis hepatocellular carcinoma if left untreated. Conventional modalities mainly symptomatic, with no definite solution. Beta-glucan-based biological response modifiers a potential strategy in lieu their beneficial metabolic effects. <i>Aureobasidium pullulans</i> strains AFO-202...
OBJECTIVES: In this pilot study, we have evaluated the specific metabolic and immune-related benefits of AFO-202 strain N-163 black yeast Aureobasidium pullulansproduced beta 1,3-1,6 glucan in healthy human subjects.METHODS: Sixteen Japanese male volunteers (aged 40 to 60 years) took part clinical trial.They were divided into four groups (n = 4 each): Group I consumed betaglucan (2 sachets 1 g each per day), IA for 35 days IB 21 days; II a combination each) beta-glucan (1 sachet 15 gel IIA...
Introduction Pancreatic cancer is an aggressive and its prognosis remains poor. Therefore, additional effective therapy required to augment and/or complement current therapy. CD147, high expression in pancreatic cancer, involved the metastatic process considered a good candidate for targeted CD147-specfic imaging could be useful selection of appropriate patients. we evaluated potential fully human anti-CD147 monoclonal antibody 059-053 as new positron emission tomography (PET) probe cancer....
Objective Pancreatic cancer is an aggressive tumor and the prognosis remains poor. Therefore, development of more effective therapy needed. We previously reported that 89Zr-labeled TSP-A01, antibody against transferrin receptor (TfR), highly accumulated in a pancreatic xenograft, but not major normal organs. In present study, we evaluated efficacy radioimmunotherapy (RIT) with 90Y-TSP-A01 mouse models. Methods TfR expression cell lines (AsPC-1, BxPC-3, MIAPaCa-2) was by immunofluorescence...
Abstract Background Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are highly prevalent conditions characterized by inflammation fibrosis of the which can progress to cirrhosis hepatocellular carcinoma if left untreated. Lifestyle disorders such as obesity, diabetes dyslipidaemia predispose associated with progression. Conventional modalities mainly symptomatic, no definite solution. Beta glucan-based biological response modifiers a potential strategy in...
Abstract Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide. Up to 20% of oral dysplasia cases have been suggested undergo malignant transformation OSCC; however, there are no methods predict OSCC development. In this study, identify genes associated with progression, we performed genomic copy number analyses DNA samples isolated from primary and via microdissection method found elevated expression transferrin receptor C (TfR1/TFRC) amplification in OSCC. The rate...
Pancreatic cancer is aggressive and its prognosis remains poor; thus, effective therapy urgently needed. Transferrin receptor (TfR) highly expressed in pancreatic considered to be a good candidate for molecular-targeted therapy. We radiolabeled evaluated fully human anti-TfR monoclonal antibodies as new PET probe evaluating the biodistribution of antibody cancer.TfR expression was four (MIAPaCa-2, PANC-1, BxPC-3, AsPC-1) murine A4 cell lines. The binding 125I-labeled (TSP-A01, TSP-A02,...
Poor sleep quality is a major problem in patients with autism spectrum disorder (ASD), and attributed to low melatonin levels. Melatonin supplementation recommended; however, its effectiveness varies. β-Glucans have previously been shown improve levels animal studies. Herein, we examined the of Aureobasidium pullulans (Nichi Glucan), species black yeast that contains beta-1,3/1,6-glucan, pilot study children ASD.Thirteen (age, 2.5-13 years) ASD were recruited for study. The control group...
Briefing of the trauma team before patient arrival is unstructured in many centers. We surveyed teams regarding agreement on care priorities and evaluated impact a structured, physician-led briefing concordance during simulated resuscitations.Trauma nurses at our Level II center were surveyed, they participated four resuscitation scenarios, randomized to "briefed" or "nonbriefed." For nonbriefed independently reviewed triage sheets with written information. Briefed scenarios had structured...
Abstract Background Imbalances in glucose and lipid metabolism the background of a declining immune system, along with aging, make one prone to glucolipotoxicity-related diseases such as hepatic steatosis high risk infection-related mortality, COVID-19, warranting safe prophylactic measure help regulate both system. Based on beneficial effects AFO-202 strain black yeast Aureobasidium pullulans -produced beta 1,3-1,6 glucan balancing blood enhancement, that N-163 same species modulation, this...
The poor prognosis of pancreatic cancer requires the development more effective therapy. CD147 expresses in with high incidence and has a crucial role invasion metastasis. We developed fully human monoclonal antibody (059-053) affinity for CD147. Here we evaluated efficacy combined treatment using radioimmunotherapy (RIT) 90Y-labeled 059-053 gemcitabine BxPC-3 xenograft mouse model. Expression matrix metalloproteinase-2 (MMP2) tumors was evaluated. In vitro vivo properties were 111In-labeled...
The search for effective antibodies (Ab) curable cancer immunotherapy has been a quest of many research groups in order to find an target that exists on the cell surface. So far there have no conclusive answers shed light search. This study therefore aimed bridge gap therapy. Screening against 49 kinds lines belonging 11 solids cancers was performed. Isolation and characterization approximately 4200 monoclonal (mAb) also performed thereafter. Of those mAb 488 clones turned out bind 29...