A5048972419- Cardiac Fibrosis and Remodeling
- Atherosclerosis and Cardiovascular Diseases
- Signaling Pathways in Disease
- Viral Infections and Immunology Research
- Aortic aneurysm repair treatments
- Lipoproteins and Cardiovascular Health
- IL-33, ST2, and ILC Pathways
- Helicobacter pylori-related gastroenterology studies
- Immune cells in cancer
- T-cell and B-cell Immunology
- Acute Myocardial Infarction Research
- Macrophage Migration Inhibitory Factor
- Protein Tyrosine Phosphatases
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Cardiomyopathy and Myosin Studies
- Microscopic Colitis
- Obesity, Physical Activity, Diet
- Clusterin in disease pathology
- Apelin-related biomedical research
- Inflammasome and immune disorders
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cardiovascular Disease and Adiposity
Huazhong University of Science and Technology
2011-2024
Union Hospital
2018-2024
Wuhan Union Hospital
2020-2024
Union Hospital
2011-2022
Peking University
2021
Peking University Third Hospital
2021
Regulatory T cells (Tregs), traditionally recognized as potent suppressors of immune response, are increasingly attracting attention because a second major function: residing in parenchymal tissues and maintaining local homeostasis. However, the existence, unique phenotype, function so-called tissue Tregs heart remain unclear.In mouse models myocardial infarction (MI), ischemia/reperfusion injury, or cardiac cryoinjury, dynamic accumulation injured myocardium was monitored. The bulk RNA...
Abstract Aims A persistent cardiac T-cell response initiated by myocardial infarction is linked to subsequent adverse ventricular remodelling and progression of heart failure. No data exist on receptor (TCR) repertoire changes in combination with phenotypic characterization T cells ischaemic failing human hearts. Methods results Analysis TCR high-throughput sequencing revealed that compared control hearts, those hearts showed a clonally expanded but similar usage patterns TRBV-J...
Overactivated inflammatory responses contribute to adverse ventricular remodeling after myocardial infarction (MI). Regulatory B cells (Bregs) are a newly discovered subset of with immunomodulatory roles in many immune and inflammation-related diseases. Our study aims determine whether the expansion Bregs exerts beneficial effect on explore mechanisms involved. Here, we showed that adoptive transfer ameliorated murine MI model, as demonstrated by improved cardiac function, decreased scar...
Epicardial adipose tissue (EAT) acts as an active immune organ and plays a critical role in the pathogenesis of heart failure (HF). However, characteristics cells EAT HF patients have rarely been elucidated.To identify key EAT, integrated bioinformatics analysis was performed on public datasets. samples with paired subcutaneous (SAT), heart, peripheral blood from were collected validation experiments. T cell receptor (TCR) repertoire assessed by high-throughput sequencing. The phenotypic...
Emerging evidence supports that intestinal microbial metabolite short-chain fatty acids (SCFAs) increase the pool of regulatory T cells (Tregs) in colonic lamina propria (cLP) and protect against nonintestinal inflammatory diseases, such as atherosclerosis post-infarction myocardial inflammation. However, whether how SCFAs inflamed aortas subjects with abdominal aortic aneurysm (AAA) remains unclear. Here, authors revealed protective effect on AAA mice expansion Tregs cLP, propionate exerted...
CD4+ T cells are abnormally activated in patients with dilated cardiomyopathy (DCM) and might be associated the immunopathogenesis of disease. However, underlying mechanisms cell activation remain largely undefined. Our aim was to investigate whether dysregulation microRNAs (miRNAs) DCM. from DCM showed increased expression levels CD25 CD69 enhanced proliferation response anti-CD3/28, indicating an state. miRNA profiling analysis magnetically sorted revealed a distinct pattern compared...
Abstract Background Both the crystalline and soluble forms of cholesterol increase macrophage secretion interleukin 1β (IL-1β), aggravating inflammatory response in atherosclerosis (AS). However, link between regulatory T cells (Tregs) remains unclear. This study aimed to investigate effect treatment on Tregs. Methods Differentiation induced Tregs (iTregs) was analyzed using flow cytometry. The expression hypoxia-inducible factor-1a (HIF-1a) its target genes measured by western blotting...
Abstract Immune cell infiltration is a significant pathological process in abdominal aortic aneurysms (AAA). T cells, particularly CD4 + are essential immune cells responsible for substantial of the aorta. Regulatory (Tregs) AAA have been identified as tissue‐specific; however, time, location, and mechanism acquiring tissue‐specific phenotype still unknown. Using single‐cell RNA sequencing (scRNA‐seq) on from aorta spleen, we discovered heterogeneity among activated, proliferating developed...
Background/Aims: Newly identified IL-10-producing regulatory B cells (Bregs) have been shown to play an important role in the suppression of immune responses. Chronic activation participates pathogenesis dilated cardiomyopathy (DCM) but whether Bregs are involved its development remains unclear. We aimed investigate circulating frequency and function DCM. Methods: In total, 35 DCM patients (20 men 15 women) 44 healthy controls (23 21 were included experiment, was detected using flow...
Observational studies indicate that insomnia may increase risk of peptic ulcer disease (PUD). Our purpose is to clarify the possible causal relationship between and PUD by Mendelian randomization analyses.We carried out analyses using summary statistics data for genetic variants reported from a GWAS (N = up 1,331,010 individuals) 456,327 individuals). Three approaches were used explore whether might play role in PUD, pathway functional enrichment conducted anticipate underlying...
Myocardial ischemia-reperfusion injury (MIRI) is common clinical complication, which represents significant challenge in the treatment of acute myocardial infarction (AMI) diseases. Interleukin 35 (IL-35) exhibits anti-inflammatory properties via engagement gp130, IL-12Rβ2 and IL-27Rα receptors. However, whether IL-35 plays a beneficial role MIRI potential underling mechanism are unclear. We showed that conferred protection from as demonstrated by reduced infarct size cardiac troponin T,...
Abdominal aortic aneurysms (AAAs) elicit massive inflammatory leukocyte recruitment to the aorta. CD4+ T cells, which include regulatory cells (Tregs) and conventional (Tconvs), are involved in progression of AAA. Tregs have been reported limit AAA formation. However, function phenotype Tconvs found AAAs remain poorly understood. We characterized by bulk RNA sequencing discovered that aneurysm highly expressed Cxcr6 Csf2. Herein, we determined CXCR6/CXCL16 signaling axis controlled...
Abstract CD4 + T‐cell counts are increased and activated in patients with chronic heart failure (CHF), whereas regulatory (Treg) expansion is inhibited, probably due to aberrant receptor (TCR) signaling. TCR signaling affected by protein tyrosine phosphatase nonreceptor type 22 (PTPN22) autoimmune disorders, but whether PTPN22 influences CHF remains unclear. This observational case–control study included 45 [18 ischemic versus 27 nonischemic (NIHF)] 16 non‐CHF controls. We used flow...
Objective: Animal studies suggest that regulatory T (T reg ) cells play a beneficial role in ventricular remodeling and our previous data have demonstrated defects of patients with chronic heart failure (CHF).However, the mechanisms behind reg-cell remained unknown.We here sought to elucidate mechanism CHF patients. Methods Results:We performed flow cytometry analysis reduced numbers peripheral blood CD4 + CD25 FOXP3 CD45RO 2 CD45RA naı ¨ve (nT memory (mT as compared non-CHF...
Objective: To analyze the impact of previous exposure to macrolide, quinolones and nitroimidazole antibiotics on eradication rate bismuth quadruple therapy (BQT) in newly diagnosed patients with Helicobacter pylori(H. pylori). Methods: A total 469 H. pylori initially treated at Third Hospital Peking University from September 2017 August 2020 were retrospectively recruited. The therapeutic regimens BQT containing clarithromycin/levofloxacin/metronidazole recommended by Chinese guidelines....
Abstract Objective: Observational studies indicate that insomnia may increase the risk of developing and/or dying from cardiovascular diseases, especially coronary artery disease (CAD). Our purpose is to explore underlying causal relationship between genetic variants susceptible and CAD by Mendelian randomization analysis. Methods: The study was conducted using publicly available statistical data on identified a genome-wide association meta-analysis ( n = 113,006 individuals) 184,305...
Abstract Background: Familial hypercholesterolemia (FH) as the most common risk factor for premature coronary artery disease (CAD), results in huge economic burden on families and society, but it could be significantly under-diagnosed. Methods: In this study, we identified a pathogenic frame shift insertion mutation LDLR (c.2517_2518insCA, p.C839fs) from Chinese FH family through whole exome sequencing (WES) sanger sequencing, which hasn't been reported before. According to 2015 ACMG/AMP,...